Bovine serum albumin: An efficient biomacromolecule nanocarrier for improving the therapeutic efficacy of chrysin

Nosrati, Hamed; Rakhshbahar, Akram; Salehiabar, Marziyeh; Afroogh, Saeed; Manjili, Hamidreza Kheiri; Danafar, Hossein; Davaran, Soodabeh

doi:10.1016/j.molliq.2018.06.066

Cited by 46 publications

(15 citation statements)

References 37 publications

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“…The MTT test showed that blank bovine serum albumin nanoparticles were highly biocompatible and do not possess a toxic effect (the determined cell viability >100%) [ 53 ]. Next, the same cell line was also used for cytotoxicity assessment for chrysin loaded bovine serum albumin nanoparticles, synthesized by a desolvation procedure, as reported by Nosrati et al In addition, in this case, a very high level of viability of these cells (>100%) was noted for nanoparticles that were not loaded [ 54 ]. In another publication, colistin-loaded human albumin nanoparticles were prepared by a purposely tuned double-emulsion method and tested on a human foreskin fibroblasts cell line.…”

Section: Resultsmentioning

confidence: 99%

The Development of the Innovative Synthesis Methodology of Albumin Nanoparticles Supported by Their Physicochemical, Cytotoxic and Hemolytic Evaluation

et al. 2021

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Many studies are being performed to develop effective carriers for controlled cytostatic delivery wherein albumin is a promising material due to its tendency to accumulate near cancer cells. The novelty of this work involves the development of the synthesis methodology of albumin nanoparticles and their biological and physicochemical evaluation. Albumin particles were obtained via the salt-induced precipitation and K3PO4 was used as a salting-out agent. Various concentrations of protein and salting-out agent solutions were mixed using a burette or a syringe system. It was proved that the size of the particles depended on the concentrations of the reagents and the methodology applied. As a result of a process performed using a burette and 2 M K3PO4, albumin spheres having a size 5–25 nm were obtained. The size of nanospheres and their spherical shape was confirmed via TEM analysis. The use of a syringe system led to preparation of particles of large polydispersity. The highest albumin concentration allowing for synthesis of homogeneous particles was 2 g/L. The presence of albumin in spheres was confirmed via the FT-IR technique and UV-Vis spectroscopy. All samples showed no cytotoxicity towards normal human dermal fibroblasts and no hemolytic properties against human erythrocytes (the hemolysis did not exceed 2.5%).

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Section: Resultsmentioning

confidence: 99%

The Development of the Innovative Synthesis Methodology of Albumin Nanoparticles Supported by Their Physicochemical, Cytotoxic and Hemolytic Evaluation

et al. 2021

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“…Despite that, a variety of phenolic compounds such as gallic acid, caffeic acid, catechin, and apigenin have also been reported in both honeys [14, 15]. Honey exhibits significant antioxidant, anticancer, and antiatherogenic activities which may be attributed partly to these compounds [16–18]. For example, SBH has been proven to show peculiar antioxidant activities and exhibits outstanding ability in reducing inflammation and infection [19].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Properties of Stingless Bee Honey in Comparison with European Bee Honey

Amin

Sabri

Mohammad

et al. 2018

Advances in Pharmacological Sciences

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Both honeybees (Apis spp.) and stingless bees (Trigona spp.) produce honeys with high nutritional and therapeutics value. Until recently, the information regarding potential health benefits of stingless bee honey (SBH) in medical databases is still scarce as compared to the common European bee honey (EBH) which is well known for their properties as therapeutic agents. Although there have been very few reports on SBH, empirically these products would have similar therapeutic quality as the EBH. In addition, due to the structure of the nest, few studies reported that the antimicrobial activity of SBH is a little bit stronger than EBH. Therefore, the composition of both the types of honey as well as the traditional uses and clinical applications were compared. The results of various studies on EBH and SBH from tissue culture research to randomised control clinical trials were collated in this review. Interestingly, there are many therapeutic properties that are unique to SBH. Therefore, SBH has a great potential to be developed for modern medicinal uses.

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“…Also, the history of allergy to albumin is rare in humans [7][8][9][10]. Bovine serum albumin (BSA) is one of the most extensively studied of this group of proteins, particularly because of its structural homology with human serum albumin, the frequency of its source, its cheap price, its easy separation and purification from the serum of bovine blood [11]. BSA nanoparticles (BSANPs) are a suitable candidate for anticancer drugs to reduce the complications of anticancer drugs due to its compatibility with the body.…”

Section: Introductionmentioning

confidence: 99%

“…The colour change was then analysed by UV-vis spectroscopy at 570 nm by Microplate reader (Biotake, USA). The cell viability value was determined as follows[27]μ % cell viability = A treated / A control × 100(11)…”

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confidence: 99%

Improvement of efficacy and decrement cytotoxicity of oxaliplatin anticancer drug using bovine serum albumin nanoparticles: synthesis, characterisation and release behaviour

Ziaaddini

Saeidifar

Eslami-Moghadam

et al. 2020

IET nanobiotechnol.

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To sustained release of an anticancer drug, oxaliplatin (OX), a non-toxic and biocompatible nanocarrier based on bovine serum albumin (BSA) were synthesised by desolvation method and characterised using Fourier-transform infrared (FTIR) spectroscopy, field emission scanning electron microscopy (FESEM), atomic force microscopy (AFM) and dynamic light scattering. The results showed that the BSA nanoparticles (BSANPs) with a mean magnitude of 187.9 ± 1.2 nm have spherical morphology with a smooth surface and a uniform distribution. Furthermore, OX was loaded onto the BSANPs and the loading was confirmed by FTIR, AFM and FESEM techniques. The percentage of encapsulation efficiency and drug loading were determined by absorption spectroscopy (UV-vis). The drug release studies showed that release of OX from BSANPs exhibited slower release rate. However, the release kinetics followed the first-order kinetic for both of them with the non-Fickian release behaviour. The electrochemical analysis showed stability of OX loaded onto the BSANPs (OX@BSANPs) and confirmed the diffusion mechanism. Furthermore, the results of MTT assay revealed increasing of normal cell viability and cancer cell death in the OX@BSANPs compared to only OX. It was shown that the BSANPs could be safely used as a biocompatible nanocarrier for the sustained release of OX. 2 Experimental 2.1 Materials BSA powder, phosphate buffered saline (PBS) tablet, dialysis bag (12 kDa), glutaraldehyde 25%, RPMI1640, 3-(4,5dimethylthialzol-a-yl)-2,5-diphenyltetrazolium bromide (MTT), foetal bovine serum (FBS), penicillin, dimethyl sulfoxide were obtained from Sigma-Aldrich Co., USA. OX anticancer drug powder was purchased from LG South Korea. Ethanol was purchased from Merck Chemical Co., Germany. HFFF2 and MCF7 cell lines were obtained from the cell bank of Pasteur institute in Tehran, Iran. All of the reagents were used as received. Deionised water was used throughout the experiment. 2.2 Methods The Fourier-transform infrared (FTIR) spectra of BSA, BSANPs and OX@BSANPs were carried out (Vector33 model, Bruker, USA) by KBr tablet and were scanned in the range of 400-40,000 cm −1. The hydrodynamic diameter and the distribution of the BSANPs were performed using dynamic light scattering (DLS)

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Bovine serum albumin: An efficient biomacromolecule nanocarrier for improving the therapeutic efficacy of chrysin

Cited by 46 publications

References 37 publications

The Development of the Innovative Synthesis Methodology of Albumin Nanoparticles Supported by Their Physicochemical, Cytotoxic and Hemolytic Evaluation

The Development of the Innovative Synthesis Methodology of Albumin Nanoparticles Supported by Their Physicochemical, Cytotoxic and Hemolytic Evaluation

Therapeutic Properties of Stingless Bee Honey in Comparison with European Bee Honey

Improvement of efficacy and decrement cytotoxicity of oxaliplatin anticancer drug using bovine serum albumin nanoparticles: synthesis, characterisation and release behaviour

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