Bovine serum albumin binding study to erlotinib using surface plasmon resonance and molecular docking methods

Taghipour, Parvin; Zakariazadeh, Mostafa; Sharifi, Maryam; Dolatabadi, Jafar Ezzati Nazhad; Barzegar, Abolfazl

doi:10.1016/j.jphotobiol.2018.04.008

Cited by 42 publications

(11 citation statements)

References 20 publications

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“…The site probe experiments with phenylbutazone suggest that the ERL interacted with BSA's subdomain IIA (Site I). However, an earlier study using molecular docking suggested that ERL binds to subdomain IB (Site III) and subdomain IIIA (site II) of BSA [53]. Furthermore, the site probe study with ibuprofen (Site II marker) did not suggest the interaction of ERL at Site II of BSA.…”

Section: Molecular Docking and Molecular Dynamic Simulation (Mds)mentioning

confidence: 88%

Interaction Characterization of a Tyrosine Kinase Inhibitor Erlotinib with a Model Transport Protein in the Presence of Quercetin: A Drug–Protein and Drug–Drug Interaction Investigation Using Multi-Spectroscopic and Computational Approaches

et al. 2022

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The interaction between erlotinib (ERL) and bovine serum albumin (BSA) was studied in the presence of quercetin (QUR), a flavonoid with antioxidant properties. Ligands bind to the transport protein BSA resulting in competition between different ligands and displacing a bound ligand, resulting in higher plasma concentrations. Therefore, various spectroscopic experiments were conducted in addition to in silico studies to evaluate the interaction behavior of the BSA-ERL system in the presence and absence of QUR. The quenching curve and binding constants values suggest competition between QUR and ERL to bind to BSA. The binding constant for the BSA-ERL system decreased from 2.07 × 104 to 0.02 × 102 in the presence of QUR. The interaction of ERL with BSA at Site II is ruled out based on the site marker studies. The suggested Site on BSA for interaction with ERL is Site I. Stability of the BSA-ERL system was established with molecular dynamic simulation studies for both Site I and Site III interaction. In addition, the analysis can significantly help evaluate the effect of various quercetin-containing foods and supplements during the ERL-treatment regimen. In vitro binding evaluation provides a cheaper alternative approach to investigate ligand-protein interaction before clinical studies.

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Section: Molecular Docking and Molecular Dynamic Simulation (Mds)mentioning

confidence: 88%

Interaction Characterization of a Tyrosine Kinase Inhibitor Erlotinib with a Model Transport Protein in the Presence of Quercetin: A Drug–Protein and Drug–Drug Interaction Investigation Using Multi-Spectroscopic and Computational Approaches

et al. 2022

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“…The shift of the SPR angle is very sensitive to the refractive index change at the metal–liquid interface, making it a powerful tool for real-time monitoring of molecular and particle binding at the interface in a label-free manner [ 43 ]. It has been used to analyze binding specificity between molecules [ 44 , 45 , 46 ], the concentration of target molecules [ 47 , 48 ], kinetic parameters of association and dissociation [ 49 , 50 ], etc. More recently, with the development of SPR microscopy as shown in Figure 1 b, which can directly monitor the nanoscale motion of single bacteria at the interface, SPR microscopy has become a powerful tool for rapid drug resistance evaluation [ 51 ].…”

Section: Surface Plasmon Resonance For Bacteria Detectionmentioning

confidence: 99%

Optical Methods for Label-Free Detection of Bacteria

et al. 2022

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Pathogenic bacteria are the leading causes of food-borne and water-borne infections, and one of the most serious public threats. Traditional bacterial detection techniques, including plate culture, polymerase chain reaction, and enzyme-linked immunosorbent assay are time-consuming, while hindering precise therapy initiation. Thus, rapid detection of bacteria is of vital clinical importance in reducing the misuse of antibiotics. Among the most recently developed methods, the label-free optical approach is one of the most promising methods that is able to address this challenge due to its rapidity, simplicity, and relatively low-cost. This paper reviews optical methods such as surface-enhanced Raman scattering spectroscopy, surface plasmon resonance, and dark-field microscopic imaging techniques for the rapid detection of pathogenic bacteria in a label-free manner. The advantages and disadvantages of these label-free technologies for bacterial detection are summarized in order to promote their application for rapid bacterial detection in source-limited environments and for drug resistance assessments.

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“…For example, carboxymethyl dextran (CMD)-coated gold SPR sensor increases the number of immobilized biomolecules. Biomolecules immobilized on the CMD surface should carry amine, carboxyl, aldehyde or thiol groups [91][92][93][94][95][96][97]. The most commonly used surface chemistry to immobilize biomolecule on CMD sensor is amine coupling process using mixture of EDC and NHS.…”

Section: Immobilization Of Biomolecules On Zno Layermentioning

confidence: 99%

ZnO for performance enhancement of surface plasmon resonance biosensor: a review

Mei

Menon

Hegde³

2020

Mater. Res. Express

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This paper reviews Kretschmann-based SPR sensor utilizing ZnO thin films and nanostructures for performance enhancement. The advancement in surface plasmon resonance technology relies on low-cost, high sensitivity and high selectivity sensor. Metal oxide has been incorporated in SPR sensor to be used for detection of biological and chemical compounds. ZnO as one of the metal oxides is an attractive material due to its unique physical and optical properties. Numerous techniques for fabrication and characterization of ZnO on SPR gold substrate have been studied. The mechanism for gas and biomolecules detection depends on their interaction with ZnO surface, which is mainly attributed to the high isoelectric point of ZnO. There are several types of ZnO nanostructures which have been employed for SPR application based on the Kretschmann configuration. In future, the thin film and nanostructures of ZnO have potential applications for miniature design, robust, high sensitivity, and low-cost portable type of SPR biosensor to be used for on-site testing in real-time and label-free manner.

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Bovine serum albumin binding study to erlotinib using surface plasmon resonance and molecular docking methods

Cited by 42 publications

References 20 publications

Interaction Characterization of a Tyrosine Kinase Inhibitor Erlotinib with a Model Transport Protein in the Presence of Quercetin: A Drug–Protein and Drug–Drug Interaction Investigation Using Multi-Spectroscopic and Computational Approaches

Interaction Characterization of a Tyrosine Kinase Inhibitor Erlotinib with a Model Transport Protein in the Presence of Quercetin: A Drug–Protein and Drug–Drug Interaction Investigation Using Multi-Spectroscopic and Computational Approaches

Optical Methods for Label-Free Detection of Bacteria

ZnO for performance enhancement of surface plasmon resonance biosensor: a review

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