This article presents the results of studies of the biochemical composition of raspberry varieties (Rubus idaeus L.) cultivated in the Orenburg region. The research was carried out on the collection plots of raspberries of the Orenburg branch of the Federal State Budgetary Research Center of Horticulture 2005-2021. The high content of soluble solids in summer raspberries, compared with the control variety Rannii Syurpriz (12.17-15.21 %), had the varieties Samarskaya Plotnaya, Juravlik, Solnishko and on average exceeded by 3.6-5.8 %, and in raspberries of the repair type of fruiting, the highest value of this indicator was noted in varieties Oranjevoe Chudo (10.7-13.0 %) and Arisha (11.4-12.8 %). The coefficient of variation is 11-16 %. On average, the sugar content in the fruits of raspberry varieties varied from 5.9 to 8.5 %, with the largest amount in the fruits of the varieties Rannii Syurpriz (K) (8.5 %), Juravlik (8.2 %) and Solnishko (8.1 %). These varieties had the most balanced taste due to the low acid content. The maximum content of titrated acid in fruits was 2.0 % in Samarskaya Plotnaya varieties and 2.1 % in Rubinovoe Ojerele varieties. Over many years of research, it has been revealed that the content of ascorbic acid in summer raspberries averaged 24.9 (Volnica) – 51.1 (Skromnica) mg/100 g, and in raspberries of the primocane raspberry of fruiting 40.0 (Jar-Ptica) – 45.5 (Gerakl) mg/100 g. A higher content of ascorbic acid in the fruits of summer raspberries, compared with the control variety Rannii Syurpriz (36.19-45.14 mg/100 g), were characterized by the varieties Skromnica (44.68-58.3 mg/100 g), Samarskaya Plotnaya (39.04-60.48 mg/100 g), Blestyaschaya (35.16-61.32 mg/100 g), Juravlik (33.37-56.90 mg/100 g). The highest content of ascorbic acid in the fruits of raspberries of the repair type of fruiting was found in the varieties Gerakl (41.3-49.6 mg/100 g) and Arisha (40.5-47.3 mg/100 g) in comparison with the control variety Jar-Ptica (35.3-44.7 mg/100 g). Th us, varieties have been selected from the genetic collection of raspberries according to a complex of biochemical characteristics for use in the breeding process: Jar-Ptica, Juravlik, Arisha.