Brevundimonas balnearis sp. nov., isolated from the well water of a thermal bath

Abstract

A novel alphaproteobacterium was isolated from the well water of a thermal bath at Budapest, Hungary. Phylogenetic analysis of the novel strain showed that this bacterium belongs to a distinct lineage among the genus Brevundimonas. Based on the 16S rRNA gene sequence strain FDRGB2bT showed the highest sequence similarity values to Brevundimonas naejangsanensis BIO-TAS2-2T (97.35 %), Brevundimonas viscosa F3T (97.28 %), Brevundimonas vesicularis LMG 2350T (97.27 %), Brevundimonas nasdae GTC 1043T (97.14 %), Brevundimonas vancanneytii LMG 2337T (97.13 %) and Brevundimonas aurantiaca DSM 4731T (97.13 %). The newly isolated bacterium was strictly aerobic, and its optimum growth occurred at 20-30 °C, between pH 8-9 and without NaCl. Movement was with a single polar flagellum, but the cells could also produce stalks. The major isoprenoid quinone of strain FDRGB2bT was Q-10, the major cellular fatty acids were C18 : 1ω7c and C16 : 0, and the polar lipid profile contained phosphatidylglycerol, diphosphatidylglycerol, two unknown phospholipids and four unknown glycolipids. The characteristic diamino acid in its cell wall is meso-diaminopimelic acid. The G+C content of DNA of the type strain was 69.8 mol%. Strain FDRGB2bT (=DSM 29841T=NCAIM B.02621T) is proposed as the type strain of a novel species with the proposed name Brevundimonas balnearis sp. nov.

Terricaulis silvestris gen. nov., sp. nov., a novel prosthecate, budding member of the family Caulobacteraceae isolated from forest soil

“…This unusual cell cycle has been studied in depth for Caulobacter crescentus [1, 2], which is a heterotypic synonym of the first described species of the family, Caulobacter vibrioides [3]. To date, the family Caulobacteraceae encompasses the five genera Aquidulcibacter [4], Asticcacaulis [5–10], Brevundimonas [11–33], Caulobacter [3, 12, 19, 34–40] and Phenylobacterium [19, 41–54]. Strains in the family have been isolated from a wide range of habitats including soil [9, 16, 34, 41, 51, 53], the rhizosphere [37–39, 49], plant roots [6, 30, 40], freshwater [3, 10, 42, 48, 55], brackish water [23], seawater [14], sediments [7, 26, 27], activated sludge [17, 23, 47], sewage [24], compost [45], blood [19, 22], a space laboratory [13], the bladder of a leech [11], a Chlorella culture [12] and cyanobacterial aggregates [4].…”

Fertoeibacter niger gen. nov., sp. nov. a novel alkaliphilic bacterium of the family Rhodobacteraceae

“…Genomic DNA was isolated from RG-N-1a T , RA-N-9 and DMA-N-7a according to the methods of Tóth et al [6] and the 16S rRNA genes were amplified as previously described by Tóth et al [7]. Purification and sequencing of PCR products were carried out by LGC Genomics (Berlin, Germany).…”

“…Colony morphology was observed on R2A agar (pH 8.5) after incubation at 28 °C for 2 days by direct observations of single colonies. The morphology of the cells (size, shape, arrangement) and growth on R2A medium (pH 8.5) at 28 °C were studied in native preparations and after Gram-staining according to the methods of Claus [48] by light-microscopy (Nikon 80i) and transmission electron microscopy (TEM) by using 7100 TEM (Hitachi) as described previously [7]. Motility was assessed in R2A medium (pH 8.5, 1 v/w% NaCl) containing 0.5% agar.…”

Living in a Puddle of Mud: Isolation and Characterization of Two Novel Caulobacteraceae Strains Brevundimonas pondensis sp. nov. and Brevundimonas goettingensis sp. nov.

“…Brevundimonas appears in various habitats such as soils, deep subseafloor sediments, activated sludge, black sand, blood, and aquatic habitats [10][11][12][13][14][15][16]. They are usually non-prosthecate motile bacteria with polar flagella with only a few sessile species [11,[16][17][18]. Abraham et al, suggest that species from the genus Brevundimonas may have lost the ability to form prosthecate during evolution or permanently migrated in the motile stage of the developmental cycle [2].…”