Brief communication. Isolation, mapping, and characterization of two barley multiovary mutants

Abstract

Mutations in homeotic genes disturb the spatial and temporal patterns of development, often leading to the appearance of tissues in abnormal locations. Many homeotic genes, involved in flower development, code for proteins with a highly conserved domain called the MADS box, which acts as a sequence-specific DNA binding protein. Two floral development mutants were isolated from a fast neutron irradiated M2 barley population. The phenotypes are multiovary, that is, stamens replaced with carpels, designated mo7a, and stamens replaced with carpels and lodicules converted to leaflike structures, designated mo6b. These phenotypes resemble the Arabidopsis mutants APETALA3 (AP3) and PISTILATA (PI). The mo6b and mo7a mutants were mapped to the centromeric region of chromosome 1 (7H) and to the telomeric region of chromosome 3 (3H), respectively.

HvSL1andHvMADS16promote stamen identity to restrict multiple ovary formation in barley

“…The present study confirms the location of the mov2 locus on the short-arm telomeric region of chromosome 3H (3HS) (Soule et al ., 2000), and additionally refines the region to a 449 Kb interval in a mov2.g (cv. Steptoe) x Morex F 3 population ( Fig.…”

“…A previous study had mapped the barley mov2 locus to the telomeric region on the short arm of chromosome 3H (3HS) within a ∼ 28 Mb genomic interval (Soule et al ., 2000). This interval is too large to reliably identify the underlying causative gene(s).…”

“…At least three multiovary loci have been reported to date (Barley Genetics Newsletter, 2013). Of these, the multiovary1 ( mov1 ) locus (Tazhin, 1980; Soule et al ., 2000) bears a striking resemblance to the rice Osmads16/superwoman1 mutant (Xiao et al ., 2009). In barley mov1 florets, lodicules are transformed into one pair of leaf-like organs and stamens show homeotic conversion into additional carpels ( Fig.…”

“…The multiovary1 (mov1) locus was initially recorded as a homeotic conversion of stamens into additional non-functional carpels, with substitution of lodicules as half-curled leaf-like structures and partial fertility (Tazhin, 1980). Linkage and mapping attempts of mov1 alleles in the barley varieties Revelatum (spontaneous mutation) and Steptoe (fastneutron mutagenesis) positioned the locus on the centromeric region of chromosome 7H and it was proposed this locus may also encode a member of the MADS-box gene family (Tazhin, 1980;Soule et al, 1996;Soule et al, 2000). Despite an early general description of the mutant phenotype and rough mapping of the mov2 and mov1 loci, the causative gene sequences have yet to be identified.…”

“…Phenotypic and molecular analysis of HvLFY revealed functional divergence relative to its orthologue from rice ( APO2 ), particularly in relation to putative gene target sequences and a lack of impact on tillering (Selva et al, 2021). The multiovary2 ( mov2 ) locus, derived from a fast neutron irradiated population generated in the early 1990s (Soule et al ., 1996), was roughly mapped to the short arm of chromosome 3H and suggested to encode a MADS-box gene (Soule et al ., 2000). The multiovary1 ( mov1 ) locus was initially recorded as a homeotic conversion of stamens into additional non-functional carpels, with substitution of lodicules as half-curled leaf-like structures and partial fertility (Tazhin, 1980).…”