Brief Report: Intestinal Dysbiosis in Ankylosing Spondylitis

Abstract

Objective. Ankylosing spondylitis (AS) is a common, highly heritable immune-mediated arthropathy that occurs in genetically susceptible individuals exposed to an unknown but likely ubiquitous environmental trigger. There is a close relationship between the gut and spondyloarthritis, as exemplified in patients with reactive arthritis, in whom a typically self-limiting arthropathy follows either a gastrointestinal or urogenital infection. Microbial involvement in AS has been suggested; however, no definitive link has been established. The aim of this study was to determine whether the gut in patients with AS carries a distinct microbial signature compared with that in the gut of healthy control subjects.Methods. Microbial profiles for terminal ileum biopsy specimens obtained from patients with recentonset tumor necrosis factor antagonist-naive AS and from healthy control subjects were generated using culture-independent 16S ribosomal RNA gene sequencing and analysis techniques. Conclusion. We show evidence for a discrete microbial signature in the terminal ileum of patients with AS compared with healthy control subjects. The microbial composition was demonstrated to correlate with disease status, and greater differences were observed between disease groups than within disease groups. These results are consistent with the hypothesis that genes associated with AS act, at least in part, through effects on the gut microbiome. Results. Our results showed that the terminal ileum microbial communities in patients with

Consumption of a Bifidobacterium bifidum Strain for 4 Weeks Modulates Dominant Intestinal Bacterial Taxa and Fecal Butyrate in Healthy Adults

“…Specifically, Rikenellaceae family members were depleted in patients who had chronic HIV infection relative to HIV-uninfected controls (46) and suppressed in IBD patients relative to healthy controls (45,47). Conversely, one study reported a higher abundance of Ruminococcaceae and Rikenellaceae and a decrease in the abundance of Prevotellaceae in the terminal ileum microbiota of subjects with ankylosing spondylitis (AS) compared with healthy controls (48).…”

The Concept of Barrier Organ Disease in the Pathogenesis of Spondyloarthritis

“…Появились новые доказательства, свидетельствую-щие в поддержку теории, что измененная синантропная микрофлора является фактором инициации и поддержа-ния хронического воспаления при таких заболеваниях, как псориаз, воспалительные заболевания кишечника (ВЗК) и СпА [8,9,[23][24][25][26].…”

“…С развитием метода секвенирования были проведены ряд исследований по изучению отклонений в разнообра-зии микрофлоры кишечника у больных анкилозирующим спондилитом (АС), псориатическим артритом (ПсА), псо-риазом и ВЗК с внекишечными проявлениями [9,23,26,[34][35][36].…”

“…Состав кишечной микрофлоры у больных с класси-ческим вариантом АС, по сравнению со здоровой популя-цией, также значительно отличается и характеризуется дисбиозом с увеличением количества бактерий пяти се-мейств: Lachnospiraceae, Ruminococcaceae, Rikenellaceae, Porphyromonadaceae и Bacteroidaceae, а также с уменьше-нием числа Veilonellaceae и Prevotellaceae [25]. В настоящее время отсутствуют убедительные доказательства роли конкретных бактерий (Klebsiella pneumoniae, Bacteroides vulgatus) в патогенезе АС [34,38,39].…”

Intestinal Dysbiosis and Potential Consequences of Microbiome-altering Antibiotic Use in the Pathogenesis of Human Rheumatic Disease

“…Novel high-throughput DNA sequencing methodologies, together with ever-expanding bioinformatics and computational capabilities, now allow for recognition of bacterial taxons without the need for tedious, inefficient culturing approaches. These efforts have led to several studies showing gut microbiome alterations (a term known as dysbiosis) characterizing multiple autoimmune rheumatic conditions, including RA 21 , AS 22 , and psoriatic arthritis 23 . One such report specifically identified a decrease in Faecalibacterium prausnitzii in the stool of children with enthesitis-related arthritis compared to controls 24 .…”