Bromide and N-acetyl-S-(n-propyl)-l-cysteine in urine from workers exposed to 1-bromopropane solvents from vapor degreasing or adhesive manufacturing

Hanley, Kevin W.; Petersen, Martin R.; Cheever, Kenneth L.; Luo, Lian

doi:10.1007/s00420-010-0524-4

Cited by 13 publications

(18 citation statements)

References 27 publications

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“…The calculated metabolic constants are based on systemic concentrations of the 1-BP and can be used for risk assessment and dosimetry for other routes of exposure. Several studies have monitored urine metabolites from animals and humans occupationally exposed to 1-BP (Hanley et al, 2006(Hanley et al, , 2009(Hanley et al, , 2010Valentine et al, 2007). Further development of the PBPK model to include the compartment of the metabolites of 1-BP is undergoing.…”

Section: Discussionmentioning

confidence: 99%

Physiologically Based Pharmacokinetic Modeling for 1-Bromopropane in F344 Rats Using Gas Uptake Inhalation Experiments

et al. 2015

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1-Bromopropane (1-BP) was introduced into the workplace as an alternative to ozone-depleting solvents and increasingly used in manufacturing industry. The potential exposure to 1-BP and the current reports of adverse effects associated with occupational exposure to high levels of 1-BP have increased the need to understand the mechanism of 1-BP toxicity in animal models as a mean of understanding risk in workers. Physiologically based pharmacokinetic (PBPK) model for 1-BP has been developed to examine 2 metabolic pathway assumptions for gas-uptake inhalation study. Based on previous gas-uptake experiments in the Fischer 344 rat, the PBPK model was developed by simulating the 1-BP concentration in a closed chamber. In the model, we tested the hypothesis that metabolism responsibilities were shared by the p450 CYP2E1 and glutathione (GSH) conjugation. The results showed that 2 metabolic pathways adequately simulated 1-BP closed chamber concentration. Furthermore, the above model was tested by simulating the gas-uptake data of the female rats pretreated with 1-aminobenzotrizole, a general P450 suicide inhibitor, or d,l-buthionine (S,R)-sulfoximine, an inhibitor of GSH synthesis, prior to exposure to 800 ppm 1-BP. The comparative investigation on the metabolic pathway of 1-BP through the PBPK modeling in both sexes provides critical information for understanding the role of p450 and GSH in the metabolism of 1-BP and eventually helps to quantitatively extrapolate current animal studies to human.

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Section: Discussionmentioning

confidence: 99%

Physiologically Based Pharmacokinetic Modeling for 1-Bromopropane in F344 Rats Using Gas Uptake Inhalation Experiments

et al. 2015

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“…This larger comprehensive study is beyond the scope of this short communication and will be reported in detail elsewhere [28,33]. The focus of this work was the development of the HPLC-MS procedure and its figures of merit.…”

Section: Methods Reproducibility Assay Sample Stability and Future Workmentioning

confidence: 99%

“…Field urine sample collection and detail has been described elsewhere by Hanley et al [28,33]. All urine was collected with appropriate human subject and IRB approvals.…”

Section: Urine Samplesmentioning

confidence: 99%

“…The third chromatogram (C) shows m/z 204 and m/z 211 for the blank pooled urine to verify that there are no interferences. The m/z = 204 AcPrCys displayed in the last chromatogram (D) was an example of results reported separately [33] for analysis conducted at this laboratory as part of recent NIOSH occupational studies of 1-BP exposure [36].…”

Section: Chromatographic Separation and Detectionmentioning

confidence: 99%

“…The current study involves development of a procedure for an exposure biomarker for 1-BP. The general utility of analytical techniques for measuring urinary markers to evaluate 1-BP exposure has led to the development of tests which include the analysis of urinary bromine [27,28], 3-bromopropionic acid [29], 1-BP [30], or mercapturic acids as stable biomarkers [31][32][33][34]. The purpose of the work described here was to design a sensitive and specific method for the quantification of AcPrCys using HPLC ESI-MS Specific Ion Monitoring (SIM).…”

Section: Introductionmentioning

confidence: 99%

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Development of an HPLC–MS procedure for the quantification of N-acetyl-S-(n-propyl)-l-cysteine, the major urinary metabolite of 1-bromopropane in human urine

Cheever

Marlow

B’Hymer

et al. 2009

Journal of Chromatography B

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Saturated Halogenated Aliphatic Hydrocarbons Two to Three Carbons

Bevan

2023

Patty's Toxicology

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The chemicals in this chapter are C 2 to C 3 aliphatic hydrocarbons containing, chlorine, bromine, or iodine. All are volatile liquids (flammable and nonflammable) at ambient temperatures, except for ethyl chloride (gas) and hexachloroethane (solid). Most, if not all, of the chemicals in this chapter are produced in closed‐system facilities, and the majority are used, primarily or exclusively, as intermediates or feedstocks to produce other chemicals. 1,1,1‐Trichloroethane is an ozone‐depleting substance and is tightly regulated under the Montreal Protocol on Substances that Deplete the Ozone Layer. Three chemicals were not included in this edition: 1,1,2,2‐tetrabromoethane, 1,2‐dibromo‐3‐chloropropane (DBCP), and ethyl iodide. DBCP has been effectively banned as a soil fumigant due to its well‐characterized toxicity, particularly on male reproduction. For the other two chemicals, the toxicity information and use are limited.

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Bromide and N-acetyl-S-(n-propyl)-l-cysteine in urine from workers exposed to 1-bromopropane solvents from vapor degreasing or adhesive manufacturing

Abstract: This study shows that urinary Br(-) and AcPrCys are useful biomarkers of workers' 1-BP exposures using analyses sensitive enough to measure low exposure jobs.

Cited by 13 publications

References 27 publications

Physiologically Based Pharmacokinetic Modeling for 1-Bromopropane in F344 Rats Using Gas Uptake Inhalation Experiments

Physiologically Based Pharmacokinetic Modeling for 1-Bromopropane in F344 Rats Using Gas Uptake Inhalation Experiments

Development of an HPLC–MS procedure for the quantification of N-acetyl-S-(n-propyl)-l-cysteine, the major urinary metabolite of 1-bromopropane in human urine

Saturated Halogenated Aliphatic Hydrocarbons Two to Three Carbons

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