Brown Pine Leaf Extract and Its Active Component Trans-Communic Acid Inhibit UVB-Induced MMP-1 Expression by Targeting PI3K

Huh, Won; Kim, Jong‐Eun; Kang, Young‐Gyu; Park, Gaeun; Lim, Tae‐Gyu; Kwon, Jung Yeon; Song, Da Young; Jeong, Eun; Lee, Charles C.; Son, Joe Eun; Seo, Soonmin; Lee, Eunjung; Kim, Jong Rhan; Lee, Chang Yong; Park, Jun Seong; Lee, Ki Won

doi:10.1371/journal.pone.0128365

Cited by 22 publications

(19 citation statements)

References 51 publications

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“…Huh et al [12] also reported that brown pine leaf extracts, including DAA and trans -communic acid, inhibit UVB-induced MMP-1 expression in a human keratinocyte cell line (HaCaT). Unlike previous studies based on biochemical assays of other antiaging biomarkers, our study focused more on the cellular efficacy of DAA on morphological changes of intact collagen fibers in a 3D human skin environment.…”

Section: Resultsmentioning

confidence: 99%

Dehydroabietic Acid Induces Regeneration of Collagen Fibers in Ultraviolet B-Irradiated Human Dermal Fibroblasts and Skin Equivalents

Park

Kang

Kim

et al. 2019

Skin Pharmacol Physiol

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Background/Aims: Dehydroabietic acid (DAA) is a natural phytochemical found in red pine trees and herbal plants. While DAA and its derivatives are known for improving diabetes and hyperlipidemia, the antiaging effect and its underlying mechanisms of DAA on skin have not been fully examined. Here, we assessed the antiaging effects of DAA on human dermal fibroblasts and skin equivalents. Methods: We investigated the effect of DAA on the secretion of type I procollagen and matrix metalloproteinase-1 (MMP-1) in ultraviolet B (UVB)-irradiated neonatal normal human dermal fibroblasts (NHDFn). Using nonlinear optical imaging techniques, we visualized quantitative and qualitative changes of collagen fibers by DAA treatment in human skin equivalent models. Results: DAA induces increases in type I procollagen secretion when treated on UVB-irradiated NHDFn. DAA also downregulates secretion of MMP-1 through the inhibition of the JNK signaling pathway. In human skin equivalent models, we successfully visualized the spatial distribution of collagen fibers in the dermis and found that quantity, diameter, and arrangement of collagen fibers in the dermis were significantly improved by DAA treatment. Conclusion: Our results suggest that DAA could be a useful agent for improving skin photoaging through the protection and regeneration of collagen fibers in skin.

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Section: Resultsmentioning

confidence: 99%

Dehydroabietic Acid Induces Regeneration of Collagen Fibers in Ultraviolet B-Irradiated Human Dermal Fibroblasts and Skin Equivalents

Park

Kang

Kim

et al. 2019

Skin Pharmacol Physiol

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“…It is generated from primary human keratinocytes on a collagen substrate containing HDFs [16,17]. It is grown at the air-liquid interface where epidermal-dermal interactions occur, and it allows full epidermal stratification.…”

Section: Resultsmentioning

confidence: 99%

Acceleration of Collagen Breakdown by Extracellular Basic pH in Human Dermal Fibroblasts

Park

Kim

et al. 2016

Skin Pharmacol Physiol

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Background/Aims: Wound healing is a complex regeneration process involving the degradation and reassembly of connective tissues and skin layers. Previous studies have shown that pH plays a signiﬁcant role in both the direct and indirect regulation of cellular processes in the wound, which, in turn, affect the wound healing process. However, the effects of pH on the collagen breakdown component of wound healing have yet to be investigated. Therefore, we investigated the induction of accelerated collagen breakdown by pH imbalance in the skin. Methods: Na⁺/H⁺ exchanger and metalloproteinase (MMP)-1 were analyzed spectrophotometrically, and the expression of collagen type-I-alpha-1 (COL1A1) and mitogen-activated protein kinase (MAPK) was measured by Western blotting. Results: Accelerated collagen breakdown induced by extracellular basic pH via the overproduction of reactive oxygen species (ROS) and MAPK signaling was examined in skin fibroblasts and in a three-dimensional human skin equivalent system. Basic pH (>7.50) upregulated MMP-1 and downregulated COL1A1 levels via ROS generation and MAPK signaling pathways. Acidic pH (<6.04) slightly upregulated MMP-1 and slightly downregulated COL1A1 levels via ROS generation and the p38 signaling pathway. Conclusion: Our results indicate that skin pH is an important effector of collagen formation in wound healing. This finding will aid in the development of new pH-targeted therapeutic strategies.

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“…Choi et al [ 8 ] reported that resveratrol decreases the activity of phosphatidylinositide 3-kinase (PI3K), an upstream kinase of Akt, in vitro . Furthermore, it has been reported that inhibition of PI3K activity decreases Akt signaling [ 6 ], and numerous other studies have reported similar findings in various cell lines [ 13 26 ]. Based on these previous reports, we propose that resveratrol might target PI3K activity to attenuate Akt signaling pathway in Huh7-HBx cells.…”

Section: Discussionmentioning

confidence: 57%

Inhibitory effects of resveratrol on hepatitis B virus X protein-induced hepatocellular carcinoma

et al. 2017

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Liver cancer occurs very frequently worldwide and hepatocellular carcinoma (HCC) accounts for more than 80% of total primary liver cancer cases. In this study, the anticarcinogenic effects of resveratrol against hepatitis B virus (HBV)-induced HCC were investigated by using HBV X-protein-overexpressing Huh7 (Huh7-HBx) human hepatoma cells. MTT assay showed that resveratrol decreased cell viability. Fluorescence-activated cell-sorter analysis showed that resveratrol induced G1 cell cycle arrest without increasing the sub-G1 phase cell population. Therefore, we evaluated its effect on regulation of cyclin D1, which is critically involved in G1/S transition. Resveratrol lowered cyclin D1 transcription. Western blot analysis of the effects of resveratrol on upstream cyclin D1 transcriptional signaling, extracellular signal-related kinase (ERK), p90RSK, Akt, and p70S6K revealed inhibition of Akt but not the ERK signaling pathway. Collectively, the results indicate that resveratrol inhibits Huh7-HBx proliferation by decreasing cyclin D1 expression through blockade of Akt signaling. We investigated the anticarcinogenic effect and mechanism of resveratrol in xenograft model mice implanted with Huh7-HBx cells. Intraperitoneal resveratrol injection reduced tumor size in the mice. Expression of survivin was reduced, but cyclin D1 was not affected. The results demonstrate that resveratrol treatment may help manage HBV-induced HCC by regulating survivin.

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Brown Pine Leaf Extract and Its Active Component Trans-Communic Acid Inhibit UVB-Induced MMP-1 Expression by Targeting PI3K

Cited by 22 publications

References 51 publications

Dehydroabietic Acid Induces Regeneration of Collagen Fibers in Ultraviolet B-Irradiated Human Dermal Fibroblasts and Skin Equivalents

Dehydroabietic Acid Induces Regeneration of Collagen Fibers in Ultraviolet B-Irradiated Human Dermal Fibroblasts and Skin Equivalents

Acceleration of Collagen Breakdown by Extracellular Basic pH in Human Dermal Fibroblasts

Inhibitory effects of resveratrol on hepatitis B virus X protein-induced hepatocellular carcinoma

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