Dehydroabietic Acid Induces Regeneration of Collagen Fibers in Ultraviolet B-Irradiated Human Dermal Fibroblasts and Skin Equivalents

Abstract

Background/Aims: Dehydroabietic acid (DAA) is a natural phytochemical found in red pine trees and herbal plants. While DAA and its derivatives are known for improving diabetes and hyperlipidemia, the antiaging effect and its underlying mechanisms of DAA on skin have not been fully examined. Here, we assessed the antiaging effects of DAA on human dermal fibroblasts and skin equivalents. Methods: We investigated the effect of DAA on the secretion of type I procollagen and matrix metalloproteinase-1 (MMP-1) in ultraviolet B (UVB)-irradiated neonatal normal human dermal fibroblasts (NHDFn). Using nonlinear optical imaging techniques, we visualized quantitative and qualitative changes of collagen fibers by DAA treatment in human skin equivalent models. Results: DAA induces increases in type I procollagen secretion when treated on UVB-irradiated NHDFn. DAA also downregulates secretion of MMP-1 through the inhibition of the JNK signaling pathway. In human skin equivalent models, we successfully visualized the spatial distribution of collagen fibers in the dermis and found that quantity, diameter, and arrangement of collagen fibers in the dermis were significantly improved by DAA treatment. Conclusion: Our results suggest that DAA could be a useful agent for improving skin photoaging through the protection and regeneration of collagen fibers in skin.

Sarmentosamide, an Anti-Aging Compound from a Marine-Derived Streptomyces sp. APmarine042

“…UVB radiation induces MMP-1 gene transcription by triggering the MAPK signaling pathway via the phosphorylation of JNK, ERK, and p38 signaling cascades [3]. In the NHDF cells used in this study, we confirmed that UVB induced JNK, ERK, and p38 phosphorylation in a time dependent manner.…”

“…Cell lysates aliquots were subjected to western blot assay as previously described [3]. Membranes were incubated with specific antibodies against JNK (#9252, Cell Signaling Technology, Beverly, MA, USA), p-JNK (#9251s), ERK (#4695 s), p-ERK (4370 s), p38 (#9212 s), p-p38 (#4511 s), and β-actin (#sc-1616, Santa Cruz Biotechnology, Dallas, TX, USA) in Tris-buffered saline (TBS) buffer containing 5% bovine serum albumin (BSA) and 0.1% Tween-20 at 4 • C overnight.…”

“…Extrinsic aging is primarily caused by exposure to environmental factors such as air pollution [1] and ultraviolet (UV) radiation [2]. UV light is composed of UVA (315-400 nm), UVB (280-315 nm), and UVC (200-280 nm), of which UVB rays are known to penetrate the epidermis and contribute to skin photoaging [3]. UVB-induced skin photoaging causes DNA damage [4] and reactive oxygen species (ROS) generation [5] and disrupts the extracellular matrix [6].…”

Protective and Anti-Aging Effects of 5 Cosmeceutical Peptide Mixtures on Hydrogen Peroxide-Induced Premature Senescence in Human Skin Fibroblasts

“…Besides cosmeceutical peptides, there are many other materials with anti-aging and protective functions. Dehydroabietic acid is a natural phytochemical which could improve skin photoaging through the protection and regeneration of collagen fibers in skin [30]. An autologous topical serum based on the plasma rich in growth factors (PRGF) technology has been developed and might counteract the harmful effects of UV radiation [31].…”

Synthetic Retinoid Seletinoid G Improves Skin Barrier Function through Wound Healing and Collagen Realignment in Human Skin Equivalents

“…The optical setup for multi-photon microscopy was as described previously [31]. To visualize collagen fibrils in the dermis of our human skin equivalent, we used second harmonic generation (SHG) imaging (excitation wavelength = 810 nm / emission wavelength = 405/10 nm).…”