Brucine PEGylated nanoemulsion: In vitro and in vivo evaluation

Elsewedy, Heba S.; Al‐Dhubiab, Bandar E.; Mahdy, Mahmoud A.; El-Nahas, Hanan M.

doi:10.1016/j.colsurfa.2020.125618

Cited by 29 publications

(21 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, the results were in a proper range that could permit intravenous administration as formerly declared by Araújo et al, who estimated that it is reasonable for intravenous administration if the viscosity is lower than 3.9 cP [ 36 ]. These outcomes were in accordance with Elsewedy et al, who assessed the viscosity of Brucine NE to be up to 3.21 cP and established that it could safely be administered via intravenous route [ 37 ]. Further, Harun et al, estimated the viscosity of cefuroxime NE to be 1.24 cP and stated that it is acceptable for parenteral delivery as it is below 3.9 cP [ 38 ].…”

Section: Resultssupporting

confidence: 84%

See 1 more Smart Citation

Date Palm Extract (Phoenix dactylifera) PEGylated Nanoemulsion: Development, Optimization and Cytotoxicity Evaluation

Khalil

Alqahtani

Darrag

et al. 2021

Plants

Self Cite

View full text Add to dashboard Cite

Date palm fruit (Phoenix dactylifera) is reputed to have numerous biological activities, including anticancer properties. To utilize the great fortune of this fruit, the current study aimed to maximize its pharmacological activity. Date palm extract (DPE) of Khalas cultivar was obtained in powder form and then was formulated into nanoemulsion (NE). The optimized DPE-NE was formulated along with its naked counterpart followed by studying their physical and chemical properties. A qualitative assessment of total serum protein associated with the surface of formulations was implemented. Studies for the in vitro release of DPE from developed NE before and after incubation with serum were investigated. Eventually, an MTT assay was conducted. Total phenolic and flavonoid contents were 22.89 ± 0.013 mg GAE/g of dry DPE and 9.90 ± 0.03 mg QE/g of dry DPE, respectively. Homogenous NE formulations were attained with appropriate particle size and viscosity that could be administered intravenously. The optimized PEGylated NE exhibited a proper particle size, PDI, and zeta potential. Total serum protein adsorbed on PEG-NE surface was significantly low. The release of the drug through in vitro study was effectively extended for 24 h. Ultimately; PEGylated NE of DPE attained significant inhibition for cancer cell viability with IC50 values of 18.6 ± 2.4 and 13.5 ± 1.8 µg/mL for MCF-7 and HepG2 cell lines, respectively. PEGylated NE of DPE of Khalas cultivar will open the gate for future adjuvants for cancer therapy.

show abstract

Section: Resultssupporting

confidence: 84%

“…The supernatant was diluted with PBS 7.4 and analyzed for their absorbance at λmax 550 nm. For control, erythrocytes were incubated only with PBS or mixing with 1 mL of Triton-X 100 (1%) to attain 100% hemoglobin release [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

Date Palm Extract (Phoenix dactylifera) PEGylated Nanoemulsion: Development, Optimization and Cytotoxicity Evaluation

Khalil

Alqahtani

Darrag

et al. 2021

Plants

Self Cite

View full text Add to dashboard Cite

show abstract

“…A Brookfield viscometer (DV-II + Pro, USA) was employed to determine the viscosity of the formulated gel, emulgel and nanoemulgel using a spindle R5 rotated at 0.5 rpm at 25 ± 0.3 °C [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

“…Samples were measured spectrophotometrically at different time intervals (0.25, 0.5, 1, 2 until 6 h) at λ max 425 nm. Each experiment was performed in triplicate [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

“…Nanoemulsions are considered drug carrier systems that are appropriate for several routes of administration including oral, parenteral and topical delivery. A nanoemulsion is a colloidal dispersion that is thermodynamically stable, formed by two immiscible liquids that are mixed together using proper surfactant to form a single phase [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enhancement of Curcumin Anti-Inflammatory Effect via Formulation into Myrrh Oil-Based Nanoemulgel

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background: Curcumin (Cur) possesses a variety of beneficial pharmacological properties including antioxidant, antimicrobial, anti-cancer and anti-inflammatory activities. Nevertheless, the low aqueous solubility and subsequent poor bioavailability greatly limits its effectiveness. Besides, the role of myrrh oil as an essential oil in treating inflammatory disorders has been recently demonstrated. The objective of the current investigation is to enhance Cur efficacy via developing Cur nanoemulgel, which helps to improve its solubility and permeability, for transdermal delivery. Methods: The formulated preparations (Cur gel, emulgel and nanoemulgel) were evaluated for their physical appearance, spreadability, viscosity, particle size, in vitro release and ex vivo drug permeation studies. The in vivo anti-inflammatory activity was estimated using the carrageenan-induced rat hind paw edema method. Results: The formulated Cur-loaded preparations exhibited good physical characteristics that were in the acceptable range of transdermal preparations. The release of Cur from gel, emulgel and nanoemulgel after 12 h was 72.17 ± 3.76, 51.93 ± 3.81 and 62.0 ± 3.9%, respectively. Skin permeation of Cur was significantly (p < 0.05) improved when formulated into nanoemulgel since it showed the best steady state transdermal flux (SSTF) value (108.6 ± 3.8 µg/cm2·h) with the highest enhancement ratio (ER) (7.1 ± 0.2). In vivo anti-inflammatory studies proved that Cur-loaded nanoemulgel displayed the lowest percent of swelling (26.6% after 12 h). Conclusions: The obtained data confirmed the potential of the nanoemulgel dosage form and established the synergism of myrrh oil and Cur as an advanced anti-inflammatory drug.

show abstract

Brucine-loaded transliposomes nanogel for topical delivery in skin cancer: statistical optimization, in vitro and dermatokinetic evaluation

et al. 2021

View full text Add to dashboard Cite

Brucine PEGylated nanoemulsion: In vitro and in vivo evaluation

Cited by 29 publications

References 46 publications

Date Palm Extract (Phoenix dactylifera) PEGylated Nanoemulsion: Development, Optimization and Cytotoxicity Evaluation

Date Palm Extract (Phoenix dactylifera) PEGylated Nanoemulsion: Development, Optimization and Cytotoxicity Evaluation

Enhancement of Curcumin Anti-Inflammatory Effect via Formulation into Myrrh Oil-Based Nanoemulgel

Brucine-loaded transliposomes nanogel for topical delivery in skin cancer: statistical optimization, in vitro and dermatokinetic evaluation

Contact Info

Product

Resources

About