BSA adsorption onto magnetic polyvinylbutyral microbeads

Abstract

Bovine serum albumin (BSA) adsorption onto novel and feasible magnetic polyvinylbutyral-based microbeads was investigated. The microbeads were made of Mowital B30HH, a commercial product, in the range 125-250 m by a modified solvent evaporation technique. Magnetite particles were embedded in the polymer structure for favorable magnetic properties, 4.80 emu/g microbeads of saturation magnetization at 6000 Gauss magnetic field. Glutaraldehyde (GA) was used as a bonding agent to increase stability and as a ligand for protein adsorption. The amount of adsorbed BSA was optimized by changing the medium pH and the initial concentrations of GA and BSA. Dynamic adsorption data of batch runs fitted best to Langmiur kinetics. The parameters q max , kЈ f and kЈ r of the model were estimated through nonlinear regression analysis as 138 mg BSA/g adsorbent, 0.058 ml/(mg BSA ⅐ min) and 0.002 min Ϫ1 , respectively, for magnetic microbeads at pH 5.0 and 25°C. The adsorbed BSA was eluted successfully at pH 8.0 and 25°C. Possibly due to surface roughness and magnetic properties, q max was found higher than the other adsorbents reported in the literature. The results denote that these microbeads can be an alternative protein adsorbent due to high adsorption capacity and rate, as well as remarkable separation characteristics.

Adsorption of bovine serum albumin on nanosized magnetic particles

“…Effect of salt concentration was evaluated at different NaCl concentrations (0, 0.05, 0.1, 0.5, 1.0, 1.5 M). BSA concentration in supernatant was measured by UVspectrometer analysis (Shimadzu UV 1601 PC) at 280 nm [17,24]. Therefore, the adsorbed mass of protein can be calculated by mass balance.…”

Immobilization of lipase onto micron-size magnetic beads

“…The mixture was shaking gently at 110 • C for 12 h. After washing with deionized water and ethanol two times, the amino-functionalized magnetic microspheres were obtained. To facilitate the covalent attachment of enzyme, the amino groups on the surface were transferred to aldehyde groups by the glutaraldehyde method [16]. After agitating at 30 • C overnight, the glutaraldehyde-activated magnetic supports were washed with deionized water three times and stored for future use.…”

Kinetics and equilibrium of desorption removal of copper from magnetic polymer adsorbent

“…Thus, the magnetic adsorbents were synthesized for the use so that a high gradient magnetic (HGM) force field can be applied to separate and recover them from the treated water after the adsorption process. Another advantage of the HGM separation is that the non-magnetic impurities can be excluded during the recovery of magnetic adsorbents [16][17][18][19][20][21].…”