BSA-stabilized manganese phosphate nanoflower with enhanced nanozyme activity for highly sensitive and rapid detection of glutathione

Dega, Naresh Kumar; Ganganboina, Akhilesh Babu; Tran, Hai Linh; Kuncoro, Eko Prasetyo; Doong, Ruey‐an

doi:10.1016/j.talanta.2021.122957

Cited by 36 publications

(24 citation statements)

References 51 publications

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“…The recoveries of the spiked samples are in the range 95.2%–108.4%, which suggests that the present method can be employed for the detection of l -Cys in real samples. The RSD values are calculated to be <10%, which are comparable to those previously reported works. ,,− These results demonstrate the satisfactory reproducibility and applicability of this novel ferrite nanozyme for l -Cys monitoring in biological samples.…”

Section: Resultssupporting

confidence: 88%

“…It is worth noting that the zeta-potential of the ferrite nanozyme after combining with TMB shows an increase to 7.37 mV (Figure S6). The increased positive charge is probably ascribed to the phenomenon that the nonoxidized TMB can carry partial positive charge in a weak acid environment (pH 3.6), 17,36 further proving the efficient combination of the ferrite nanozyme and the substrate. The surface charges can inhibit the agglomeration of the nanosized ferrite nanozyme, thus leading to the enlarged specific surface area.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Among various nanozymes, peroxidases are most widely used to detect hydrogen peroxide, glucose, cholesterol, glutathione, and other macromolecules in body fluids. − As a substitute of natural enzymes, most peroxidase-like nanozymes follow a similar catalytic mechanism. The complex valence states of nanozymes are considered to be an important reason for its ability to simulate the catalytic behavior of natural enzymes.…”

Section: Introductionmentioning

confidence: 99%

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Mechanism and Application of Surface-Charged Ferrite Nanozyme-Based Biosensor toward Colorimetric Detection of l-Cysteine

Liu

Chen

et al. 2022

Langmuir

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Peroxidase-like nanozymes with robust catalytic capacity and detection specificity have been proposed as substitutes to natural peroxidases in biochemical sensing. However, the catalytic activity enhancement, detection mechanism, and application of nanozyme-based biosensors toward L-cysteine (L-Cys) detection still remain significant challenges. In this work, a doped ferrite nanozyme with well-defined structure and surface charges is fabricated by a two-step method of continuous flow coprecipitation and high-temperature annealing. The resulted ferrite nanozyme possesses an average size of 54.5 nm and a zeta-potential of 6.45 mV. A high-performance biosensor is manufactured based on the peroxidase-like catalytic feature of the doped ferrite. The ferrite nanozyme can oxidize the 3,3′,5,5′tetramethylbenzidine (TMB) with the assistance of H 2 O 2 because of the instinctive capacity to decompose H 2 O 2 into •OH. The Michaelis−Menten constants (0.0911 mM for TMB, 0.140 mM for H 2 O 2 ) of the ferrite nanozyme are significantly smaller than those of horseradish peroxidase. A reliable colorimetric method is established to selectively analyze L-Cys via a facile mixing-anddetecting methodology. The detection limit and linear range are 0.119 μM and 0.2−20 μM, respectively. Taking the merits of the ferrite nanozyme-based biosensors, the L-Cys level in the human serum can be qualitatively detected. It can be anticipated that the surface-charged ferrite nanozyme shows great application prospects in the fields of bioanalytical chemistry and point-of-care testing.

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Section: Resultssupporting

confidence: 88%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mechanism and Application of Surface-Charged Ferrite Nanozyme-Based Biosensor toward Colorimetric Detection of l-Cysteine

Liu

Chen

et al. 2022

Langmuir

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“…[ 47 ] The MnPNF nanozyme prepared by researchers can oxidize GSH to GSSG with the support of H 2 O 2 , which belongs to a specific class of enzymes derived from the mechanism of action of POD‐like activity, GPx‐like. [ 74 ] In a study, researchers used MnO 2 to prepare nanowire M13‐E4@MnO 2 NWs with POD‐like activity, which catalyzes the electron‐deprivation conversion of H 2 O 2 to water. The substrate 2,2'‐azino‐bis (3‐ethylbenzo‐thiazoline‐6‐sulfonic acid) diammonium salt (ABTS) was converted to electron‐deprived oxidized ABTS [ 71 ]

\begin{equation} {{\rm{H}}}_2{{\rm{O}}}_2 + {\rm{M}} \stackrel{\rm{POD}} {\hbox{\rightarrowfill}} {{\rm{H}}}_2{\rm{O}} + {\rm{ox}} - {\rm{M}}\end{equation}

…”

Section: Catalytic Mechanism Of Mn‐based Nanozymesmentioning

confidence: 99%

Manganese‐Based Nanozymes: Preparation, Catalytic Mechanisms, and Biomedical Applications

Tang

Zhang

Sun

et al. 2022

Adv Healthcare Materials

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Manganese (Mn) has attracted widespread attention due to its low-cost, nontoxicity, and valence-rich transition. Various Mn-based nanomaterials have sprung up and are employed in diverse fields, particularly Mn-based nanozymes, which combine the physicochemical properties of Mn-based nanomaterials with the catalytic activity of natural enzymes, and are attracting a surge of research, especially in the field of biomedical research. In this review, the typical preparation strategies, catalytic mechanisms, advances and perspectives of Mn-based nanozymes for biomedical applications are systematically summarized. The application of Mn-based nanozymes in tumor therapy and sensing detection, together with an overview of their mechanism of action is highlighted. Finally, the prospective directions of Mn-based nanozymes from five perspectives: innovation, activity enhancement, selectivity, biocompatibility, and application broadening are discussed.

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“…Therefore, it is an intriguing topic to develop peroxidase mimics as an alternative to the natural enzyme. Recently, considerable effort has been contributed to constructing nanomaterials-based peroxidase mimics, such as metallic oxides and salts [9][10][11], some noble metals, including Pt [12], Pd [13], and Au@Pt nanoparticles [14], layered double hydroxides [15], and quantum dots [16,17]. However, the large-scale application of nanomaterials-based enzyme mimics is hampered by several constraints, such as environmental pollution, weak enzyme-like activity, poor biocompatibility, heavy reliance on the skilled technician and sophisticated apparatus, and unavoidable enzyme-like activity variance between batches [18,19].…”

Section: Introductionmentioning

confidence: 99%

Investigation on the Peroxidase-like Activity of Vitamin B6 and Its Applications in Colorimetric Detection of Hydrogen Peroxide and Total Antioxidant Capacity Evaluation

Chen

Zhang

et al. 2022

Molecules

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The peroxidase-like activity of vitamin B6 (VB6) was firstly demonstrated by catalyzing the peroxidase chromogenic substrate 3,3’,5,5’-tetramethylbenzidine (TMB) at the existence of H2O2. The influence of different factors on the catalytic property of VB6, including pH, temperature, VB6 concentration, and incubation time, were investigated. The steady-state kinetic study results indicate that VB6 possesses higher affinity to H2O2 than natural horseradish peroxidase and some other peroxidase mimics. Besides, the radical quenching experiment results confirm that hydroxyl radical (•OH) accounts for the catalytic process. Based on the excellent peroxidase-like catalytic activity of VB6, the colorimetric methods for H2O2 and gallic acid (GA) detection were developed by measuring the absorbance variance of the catalytic system. Under the optimal conditions, the linear ranges of the methods for H2O2 and GA determination with good selectivity are 50.0–600.0 μM and 10.0–50.0 μM, respectively. In addition, the developed method was applied in the detection of H2O2 in milk samples and evaluation of total antioxidant capacity of different tea infusions. This study may broaden the application prospect of VB6 in environmental and biomedical analysis fields, contribute to profound insight of the physiological functions of VB6, as well as lay foundation for further excavation of small-molecule peroxidase mimics.

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BSA-stabilized manganese phosphate nanoflower with enhanced nanozyme activity for highly sensitive and rapid detection of glutathione

Cited by 36 publications

References 51 publications

Mechanism and Application of Surface-Charged Ferrite Nanozyme-Based Biosensor toward Colorimetric Detection of l-Cysteine

Mechanism and Application of Surface-Charged Ferrite Nanozyme-Based Biosensor toward Colorimetric Detection of l-Cysteine

Manganese‐Based Nanozymes: Preparation, Catalytic Mechanisms, and Biomedical Applications

Investigation on the Peroxidase-like Activity of Vitamin B6 and Its Applications in Colorimetric Detection of Hydrogen Peroxide and Total Antioxidant Capacity Evaluation

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