Buckwheat (Fagopyrum esculentum Moench) low molecular weight seed proteins are restricted to the embryo and are not detectable in the endosperm

Licen, M.; Kreft, Ivan

doi:10.1016/j.plaphy.2005.08.002

Cited by 15 publications

(9 citation statements)

References 30 publications

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“…1C) suggested that nFag t 2 was expressed in TB seeds abundantly and Fag t 2 only presents in albumin and is acid stable. Meanwhile, Western blotting of embryo and endosperm proteins revealed that Fag t 2 was restricted to embryo, which was consistent with the previous observations (5). One extra band was detected below the 16‐kDa target (Fig.…”

Section: Ige‐binding Capacity Of Fag T 2 By Competitive Elisa*supporting

confidence: 92%

Molecular cloning and characterization of Fag t 2: a 16-kDa major allergen from Tartary buckwheat seeds

Chen¹,

Guo²,

Qiu³

et al. 2011

Allergy

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Section: Ige‐binding Capacity Of Fag T 2 By Competitive Elisa*supporting

confidence: 92%

Molecular cloning and characterization of Fag t 2: a 16-kDa major allergen from Tartary buckwheat seeds

Chen¹,

Guo²,

Qiu³

et al. 2011

Allergy

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“…confirmed this information and showed another allergic protein of 15 kDa. Moreover, the proteins of 24, 19, 16, 9 kDa are known as strong allergens (Ličen & Kreft 2005).…”

Section: Buckwheat Proteins In the Aspect Of Allergic Reactionsmentioning

confidence: 99%

Buckwheat grains and buckwheat products - nutritional and prophylactic value of their components - a review

Christa¹,

2008

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Christa K., Soral-Śmietana M. (2008): Buckwheat grains and buckwheat products -nutritional and prophylactic value of their components -a review. Czech J. Food Sci., 26: 153-162.Buckwheat is introduced into the diet as an alternative crop of renewed interest due to its nutritive and healthpromoting value. Experiments with animal models have demonstrated that buckwheat flour may alleviate diabetes, obesity, hypertension, and hypercholesterolemia. A number of nutraceutical compounds exist in buckwheat grains and other tissues. These are a rich source of starch, proteins, antioxidants, and dietary fibre as well as trace elements. The biological value (BV) of buckwheat proteins is comparable to BV of other protein sources. Besides high-quality proteins, buckwheat grains contain some components with prophylactic value: flavonoids, fagopyrins, or thiaminbinding proteins. For the food industry, buckwheat grains are a valuable raw material to be used for the production of functional foods. Buckwheat flour may be a valuable and important ingredient in diets or food products, taking into consideration its nutritive value and potential promotion of human health.

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“…They are mainly confined to the development of molecular markers based on anonymous DNA sequences (RAPD, SSR, AFLP) and the characterization of several proteins [4-6]. Also, the molecular systematics of the genus Fagopyrum was extensively studied using nuclear and plastid sequences [7-9], and a complete sequence of the chloroplast genome of the common buckwheat was reported recently [10].…”

Section: Introductionmentioning

confidence: 99%

De novo sequencing and characterization of floral transcriptome in two species of buckwheat (Fagopyrum)

et al. 2011

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BackgroundTranscriptome sequencing data has become an integral component of modern genetics, genomics and evolutionary biology. However, despite advances in the technologies of DNA sequencing, such data are lacking for many groups of living organisms, in particular, many plant taxa. We present here the results of transcriptome sequencing for two closely related plant species. These species, Fagopyrum esculentum and F. tataricum, belong to the order Caryophyllales - a large group of flowering plants with uncertain evolutionary relationships. F. esculentum (common buckwheat) is also an important food crop. Despite these practical and evolutionary considerations Fagopyrum species have not been the subject of large-scale sequencing projects.ResultsNormalized cDNA corresponding to genes expressed in flowers and inflorescences of F. esculentum and F. tataricum was sequenced using the 454 pyrosequencing technology. This resulted in 267 (for F. esculentum) and 229 (F. tataricum) thousands of reads with average length of 341-349 nucleotides. De novo assembly of the reads produced about 25 thousands of contigs for each species, with 7.5-8.2× coverage. Comparative analysis of two transcriptomes demonstrated their overall similarity but also revealed genes that are presumably differentially expressed. Among them are retrotransposon genes and genes involved in sugar biosynthesis and metabolism. Thirteen single-copy genes were used for phylogenetic analysis; the resulting trees are largely consistent with those inferred from multigenic plastid datasets. The sister relationships of the Caryophyllales and asterids now gained high support from nuclear gene sequences.Conclusions454 transcriptome sequencing and de novo assembly was performed for two congeneric flowering plant species, F. esculentum and F. tataricum. As a result, a large set of cDNA sequences that represent orthologs of known plant genes as well as potential new genes was generated.

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Buckwheat (Fagopyrum esculentum Moench) low molecular weight seed proteins are restricted to the embryo and are not detectable in the endosperm

Cited by 15 publications

References 30 publications

Molecular cloning and characterization of Fag t 2: a 16-kDa major allergen from Tartary buckwheat seeds

Molecular cloning and characterization of Fag t 2: a 16-kDa major allergen from Tartary buckwheat seeds

Buckwheat grains and buckwheat products - nutritional and prophylactic value of their components - a review

De novo sequencing and characterization of floral transcriptome in two species of buckwheat (Fagopyrum)

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