Building a nuclear envelope at the end of mitosis: coordinating membrane reorganization, nuclear pore complex assembly, and chromatin de-condensation

Schooley, Allana; Vollmer, Benjamin; Antonin, Wolfram

doi:10.1007/s00412-012-0388-3

Cited by 72 publications

(63 citation statements)

References 168 publications

(231 reference statements)

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“…Generally, this finding mirrors the real situation in biological membranes interwoven by integral membrane proteins (see e.g. Schooley et al 2012). Similarly, we found not only NPC anchorage as a protein complex inserted into the pore membrane but we noted also frequent insertions of large complexes into the inner nuclear membrane from the nuclear side (e.g.…”

Section: Discontinuity Of Biological Membranessupporting

confidence: 87%

Spoke ring and anchorage of nuclear pore complex revealed by high resolution transmission electron microscopy

Prachar

2014

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Abstract. Using several different methods, the nuclear pore complex (NPC) was shown to be anchored in the nuclear envelope into the specific curved region, called pore membrane. Three transmembrane nucleoporins in the equatorial region of NPC contain hydrophobic stretches, which exhibit the ability to intersect the phospholipid bilayer. Using transmission electron microscopy, we observed three different evaluable morphological situations in the section through the NPC spoke ring (SR). We suppose that some sections are directed through one type of subunit that is responsible for anchoring. Other sections are directed through the second type of subunit that may provide pore membrane bending. Finally, the spoke ring is sectioned between aforementioned subunits where the pore membrane is best preserved. The proposed anchor is represented by the chains of protein complexes which replace phospholipid bilayer in a relatively large area. Second subunit, presumed bending module is represented by the bundles of chains copying the shape of the pore membrane from the side of the NPC. This work is based on very high resolution resulting in unique and complicated images of tangled and cut off protein chains, nevertheless, it provides insight into how some proteins interact with or replace the membrane.

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Section: Discontinuity Of Biological Membranessupporting

confidence: 87%

Spoke ring and anchorage of nuclear pore complex revealed by high resolution transmission electron microscopy

Prachar

2014

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“…Two models for mitotic NPC assembly have been proposed (for a review, see Schooley et al, 2012). According to the insertion model, NPCs are reassembled into an intact nuclear envelope (Macaulay and Forbes, 1996;Fichtman et al, 2010;Lu et al, 2011).…”

Section: Npc Assemblymentioning

confidence: 99%

Perforating the nuclear boundary – how nuclear pore complexes assemble

Weberruß

Antonin

2016

Journal of Cell Science

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The nucleus is enclosed by the nuclear envelope, a double membrane which creates a selective barrier between the cytoplasm and the nuclear interior. Its barrier and transport characteristics are determined by nuclear pore complexes (NPCs) that are embedded within the nuclear envelope, and control molecular exchange between the cytoplasm and nucleoplasm. In this Commentary, we discuss the biogenesis of these huge protein assemblies from approximately one thousand individual proteins. We will summarize current knowledge about distinct assembly modes in animal cells that are characteristic for different cell cycle phases and their regulation.

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“…In metazoa, the nuclear envelope breaks down during mitosis in order to facilitate the capture of highly condensed chromosomes and their segregation by the spindle apparatus. The complex architecture of the interphasic nucleus must therefore be reestablished upon mitotic exit when the nuclear envelope and pore complexes reassemble on the decondensing chromatin (for reviews see Güttinger et al, 2009;Schooley et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

The lysine demethylase LSD1 is required for nuclear envelope formation at the end of mitosis

Schooley

Moreno-Andrés

Magistris

et al. 2015

Journal of Cell Science

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The metazoan nucleus breaks down and reassembles during each cell division. Upon mitotic exit, the successful reestablishment of an interphase nucleus requires the coordinated reorganization of chromatin and formation of a functional nuclear envelope. Here, we report that the histone demethylase LSD1 (also known as KDM1A) plays a crucial role in nuclear assembly at the end of mitosis. Downregulation of LSD1 in cells extends telophase and impairs nuclear pore complex assembly. In vitro, LSD1 demethylase activity is required for the recruitment of MEL28 (also known as ELYS and AHCTF1) and nuclear envelope precursor vesicles to chromatin, crucial steps in nuclear reassembly. Accordingly, the formation of a closed nuclear envelope and nuclear pore complex assembly are impaired upon depletion of LSD1 or inhibition of its activity. Our results identify histone demethylation by LSD1 as a new regulatory mechanism linking the chromatin state and nuclear envelope formation at the end of mitosis.

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Building a nuclear envelope at the end of mitosis: coordinating membrane reorganization, nuclear pore complex assembly, and chromatin de-condensation

Cited by 72 publications

References 168 publications

Spoke ring and anchorage of nuclear pore complex revealed by high resolution transmission electron microscopy

Spoke ring and anchorage of nuclear pore complex revealed by high resolution transmission electron microscopy

Perforating the nuclear boundary – how nuclear pore complexes assemble

The lysine demethylase LSD1 is required for nuclear envelope formation at the end of mitosis

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Building a nuclear envelope at the end of mitosis: coordinating membrane reorganization, nuclear pore complex assembly, and chromatin de-condensation

Cited by 72 publications

References 168 publications

Spoke ring and anchorage of nuclear pore complex revealed by high resolution transmission electron microscopy

Spoke ring and anchorage of nuclear pore complex revealed by high resolution transmission electron microscopy

Perforating the nuclear boundary – how nuclear pore complexes assemble

The lysine demethylase LSD1 is required for nuclear envelope formation at the end of mitosis

Contact Info

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Spoke ring and anchorage of nuclear pore complex revealed by high resolution transmission electron microscopy

Spoke ring and anchorage of nuclear pore complex revealed by high resolution transmission electron microscopy