Bulb Growth in Lily Regenerated in Vitro

Langens-Gerrits, Merel; Lilien-Kipnis, H.; Croes, T.; Miller, William B.; Kollfiffel, C.; Klerk, G.J.M. de

doi:10.17660/actahortic.1997.430.39

Cited by 19 publications

(14 citation statements)

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“…Similar observations have been reported in previous studies on other Allium species (Keller et al 1993;Bonnier and Van Tuyl 1997;Le Guen-Le Saos et al 2002). In this study, 12% (w/v) sucrose was found to be optimal for bulblet growth, and that was probably attributed to an increase in the synthesis of starch and total carbohydrates (Langens-Gerrits et al 1997).…”

Section: Effect Of Temperature and Sucrose On In Vitro Bulblet Formationmentioning

confidence: 55%

Effect of plant growth regulators, temperature and sucrose on shoot proliferation from the stem disc of Chinese jiaotou (Allium chinense) and in vitro bulblet formation

Zhang

Kim

et al. 2008

Acta Physiol Plant

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In vitro shoot proliferation from stem disc of Allium chinense, a vegetatively propagated plant, was investigated in this experiment. In the present study, shoots were formed directly on stem discs on a medium containing 1 mg/l N 6 -benzyladenine (BA) and 0.5 mg/lanaphthaleneacetic acid (NAA). These shoots were further cultured on MS media supplemented with various levels of BA in combination with NAA, and new shoot clusters developed easily from the explants cultured despite considerable differences in the induction of shoot clusters with different levels of BA and NAA. The most productive combination of growth regulators proved to be 1.0 mg/l BA and 1.0 mg/l NAA, in which about 17 shoots were produced per cluster in 8 weeks culture. Most of the formed shoots were rooted 15 days after being cultured on MS media supplemented with 0.1-1.0 mg/l NAA. The survival rate of the plantlets under ex vitro conditions was 95% in pots filled with a peat: sand (2:1 v/v) mixture after two weeks. In vitro bulblet formation were strongly promoted by the high temperature of 30°C compared to that at 25, 20 and 15°C, and 12% (w/v) sucrose appeared to be optimal for bulblet development. Results from this study demonstrated that A. chinense could be in vitro propagated by using stem discs and in vitro bulblet formation could be achieved.

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Section: Effect Of Temperature and Sucrose On In Vitro Bulblet Formationmentioning

confidence: 55%

Effect of plant growth regulators, temperature and sucrose on shoot proliferation from the stem disc of Chinese jiaotou (Allium chinense) and in vitro bulblet formation

Zhang

Kim

et al. 2008

Acta Physiol Plant

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“…Similarly, the larger bulblet formation on the full-scale explants is mainly due to the reserve starch content compared with the bulblets on the small basal explants in vitro. Hence, planting bulblets (in soil) produced in vitro has shown that small bulblets emerge and grow more slowly (Lian et al, 2003), and the growth after planting greatly depends on the initial bulb weight (Langens-Gerrits et al, 1996b) due to sucrose storage in the bulblets. In addition, sprouting type is directly related to a switch in ontogenetic development from juvenile to adult vegetative ( Figure 6).…”

Section: Discussionmentioning

confidence: 99%

“…The initial size of the bulblets produced in vitro strongly affects growth and morphogenesis rates but also the transition between various vegetative and reproductive phases during development after planting. Lily bulbs of different grades differ widely in their relative growth potential and productivity in the field or greenhouse because growth after planting is determined by the initial bulb weight (Kumar et al, 2001;Langens-Gerrits et al, 1996b). Hence, the growth of bulblets that are being produced is crucial.…”

Section: Introductionmentioning

confidence: 99%

“…Hence, the growth of bulblets that are being produced is crucial. However, there are few studies available that clarify the growth of lily bulbs as a model for bulbous crops (De Klerk, 2009;Lian et al, 2003;Langens-Gerrits et al, 2003b;Langens-Gerrits et al, 1996b;De Klerk et al, 1992). Specifically, there are relatively few studies on a) the effect of different sucrose concentrations on bulb formation and the growth of bulbous plants and b) the effect of tissue culture bulblets on season performance in soil.…”

Section: Introductionmentioning

confidence: 99%

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Factors affecting bulblet growth of Lilium sp. in vitro and in vivo

Islam¹,

Roni²,

Shimasaki³

2017

Plant Omics

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The major problem with in vitro propagation of lilies is small bulblet size, and the initial size of bulblets not only strongly affects growth and morphogenesis rates but also the transition between various vegetative and reproductive phases during development after planting. Therefore, bulblet growth is the most important factor in understanding how lily bulblets grow in vitro and the role of starch converted from sucrose in the medium on the growth mechanism in lilies, with the aim of revealing the effective in vitro culture condition to enhance in vivo performance of lily bulblets. The results of this study show bulblet growth correlates with the use of starch granule reserves inside explant tissue and in the medium. The main factor determining the growth of bulblets in vitro is explant size due to internal storage of starch, which plays a vital role in bulblet growth irrespective of cultivar. Furthermore, internal storage of sucrose plays a vital role and influences regeneration and bulblet growth in both in vitro and in vivo cultures. Therefore, the size of the bulblets produced in vitro strongly affects performance after planting. There is a high correlation (R 2 = 0.9672) between bulb weight after the growth season and initial bulb weight and therefore, the ontogenetic age of bulblets at planting. Hence, culturing lily scale explants in a high sucrose concentration during regeneration of bulblets in vitro to increase bulblet size is the main point of interest for future production.

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“…This will result in efficient propagation, because lily bulbs have the capacity to regenerate bulblets at the base of their excised scale ( Figure 3B). Many studied have aimed to enhance some physiological traits of lily bulblets by regenerating the bulblets in vitro, including biomass production, repressing dormancy induction and stimulating phase change, (Bonnier and Van Tuyl 1997;Langens-Gerrits et al 1997;Langens-Gerrits 2003;Suh and Roh 2014;Gerrits and de Klerk 1992;Jásik and de Klerk 2006;Askari et al 2016).…”

Section: Native and Artificial Vegetative Propagation In Lily Bulbsmentioning

confidence: 99%

Mechanisms of vegetative propagation in bulbs : a molecular approach

Moreno-Pachon

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Bulb Growth in Lily Regenerated in Vitro

Cited by 19 publications

References 0 publications

Effect of plant growth regulators, temperature and sucrose on shoot proliferation from the stem disc of Chinese jiaotou (Allium chinense) and in vitro bulblet formation

Effect of plant growth regulators, temperature and sucrose on shoot proliferation from the stem disc of Chinese jiaotou (Allium chinense) and in vitro bulblet formation

Factors affecting bulblet growth of Lilium sp. in vitro and in vivo

Mechanisms of vegetative propagation in bulbs : a molecular approach

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