BURP domain-containing genes in legumes: genome-wide identification, structure, and expression analysis under stresses and development

Chitkara, Pragya; Poddar, Nikita; Singh, Amarjeet; Kumar, Shailesh

doi:10.1007/s11816-022-00752-2

Cited by 7 publications

(4 citation statements)

References 48 publications

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“…These findings indicate that the majority of GmCYPs are likely to be originated from gene duplication events. However, in all homologous gene pairs, Ka/Ks ratio was observed to be less than 1, indicating duplicated genes underwent purifying selection throughout their evolutionary history (Kondrashov et al, 2002;Chitkara et al, 2022).…”

Section: Figurementioning

confidence: 93%

Comprehensive and evolutionary analysis of Spodoptera litura-inducible Cytochrome P450 monooxygenase gene family in Glycine max elucidate their role in defense

Yadav,

Panwar,

Rustagi

et al. 2023

Front. Plant Sci.

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Plants being sessile organisms and lacking both circulating phagocytic cells and somatic adaptive immune response, have thrived on various defense mechanisms to fend off insect pests and invasion of pathogens. CYP450s are the versatile enzymes, which thwart plants against insect pests by ubiquitous biosynthesis of phytohormones, antioxidants, and secondary metabolites, utilizing them as feeding deterrents and direct toxins. Therefore, a comprehensive analysis of biotic stress-responsive CYPs from Glycine max was performed to ascertain their function against S. litura-infestation. Phylogenetic analysis and evolutionary studies on conserved domains and motifs disclosed the evolutionary correspondence of these GmCYPs with already characterized members of the CYP450 superfamily and close relatedness to Medicago truncatula. These GmCYPs were mapped on 13 chromosomes; they possess 1-8 exons; they have evolved due to duplication and are localized in endoplasmic reticulumn. Further, identification of methyl-jasmonate, salicylic acid, defense responsive and flavonoid biosynthesis regulating cis-acting elements, their interaction with biotic stress regulating proteins and their differential expression in diverse types of tissues, and during herbivory, depicted their responsiveness to biotic stress. Three-dimensional homology modelling of GmCYPs, docking with heme cofactor required for their catalytic activity and enzyme-substrate interactions were performed to understand the functional mechanism of their action. Moreover, to gain insight into their involvement in plant defense, gene expression analysis was evaluated, which revealed differential expression of 11 GmCYPs upon S. litura-infestation, 12 GmCYPs on wounding while foliar spray of ethylene, methyl-jasmonate and salicylic acid differentially regulated 11 GmCYPs, 6 GmCYPs, and 10 GmCYPs respectively. Our study comprehensively analysed the underlying mechanism of GmCYPs function during S. litura-infestation, which can be further utilized for functional characterization to develop new strategies for enhancing soybean resistance to insect pests.

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Section: Figurementioning

confidence: 93%

Comprehensive and evolutionary analysis of Spodoptera litura-inducible Cytochrome P450 monooxygenase gene family in Glycine max elucidate their role in defense

Yadav,

Panwar,

Rustagi

et al. 2023

Front. Plant Sci.

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“…Formerly characterized OSCA protein sequences of Arabidopsis thaliana and Oryza sativa were retrieved from UniProt (Swiss-Prot) ( https://www.uniprot.org ), and homology search was performed using the BLAST tool (at E -value of 1e−5) against the proteome of all four aforementioned legume plants. Significant hits were selected based on ≥ 50% identity and ≥ 100 amino acid length alignment 38 , 39 . After that, all four sets of putative candidates were screened using CD-hit 40 , followed by manual curation to remove redundant sequences.…”

Section: Methodsmentioning

confidence: 99%

Genome-wide characterization and comparative analysis of the OSCA gene family and identification of its potential stress-responsive members in legumes

Chakraborty

Gangwar

Zahra

et al. 2023

Sci Rep

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Cicer arietinum, Cajanus cajan, Vigna radiata, and Phaseolus vulgaris are economically important legume crops with high nutritional value. They are negatively impacted globally by different biotic and abiotic stresses. Hyperosmolality-gated calcium-permeable channels (OSCA) have been characterized as osmosensors in Arabidopsis thaliana but have not previously reported in legumes. This study provides a genome-wide identification, characterization, and comparative analysis of OSCA genes in legumes. Our study identified and characterized 13 OSCA genes in C. cajan, V. radiata, P. vulgaris, and 12 in C. arietinum, classified into four distinct clades. We found evidence to suggest that the OSCAs might be involved in the interaction between hormone signalling pathways and stress signalling pathways. Furthermore, they play a major role in plant growth and development. The expression levels of the OSCAs vary under different stress conditions in a tissue-specific manner. Our study can be used to develop a detailed understanding of stress regulatory mechanisms of the OSCA gene family in legumes.

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“…2 The phylogenetic tree of BURP proteins and ZjBURP classification. The protein sequences from Brachypodium distachyon (10), Setaria italica (13), Oryza sativa (12), Sorghum bicolor (10), Zea mays (9), Arabidopsis thaliana (5), Glycine max (21), Cucumis sativus (6), Citrus sinensis (6), Brassica rapa (9), Populus trichocarpa (16), Vicia faba (1), and Ziziphus jujuba (17) were aligned using MUSCLE algorithm in MEGA-X, and the phylogenetic tree was constructed by MEGA-X with the neighbor-joining (NJ) method and 1,000 bootstrap replicates Fig. 3 The phylogenetic relationships, gene structure, and conserved motifs of ZjBURPs.…”

Section: Analysis Of Cis-elements In Zjburp Promoter Regionsmentioning

confidence: 99%

“…Some studies proved that a stress-responsive gene family encoding BURP domain-containing protein is plant-specific and performs important functions in plant development and stress response [3,4]. The BURP proteins contain a BURP domain with conserved structures of CHX 10 CHX 23-37 CHX 23-26 CHX 8 W [5] and has been identified in some plant species, including rice (Oryza sativa) [6], soybean (Glycine max) [7], maize (Zea mays) [8], poplar (Populus trichocarpa) [9], cotton (Gossypium Open Access *Correspondence: xingangle@nwsuaf.edu.cn raimondii, Gossypium arboretum, and Gossypium hirsutum) [10], common bean (Phaseolus vulgaris) [11], Chinese rose (Rosa chinensis) [12], and legumes (Phaseolus vulgaris, Cicer arietinum, Cajanus cajan, and Vigna radiata) [13].…”

Section: Introductionmentioning

confidence: 99%

Identification and expression analysis of BURP domain-containing genes in jujube and their involvement in low temperature and drought response

Wang

Zhang

2022

BMC Genomics

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Background Plant-specific BURP domain-containing genes are involved in plant development and stress responses. However, the role of BURP family in jujube (Ziziphus jujuba Mill.) has not been investigated. Results In this study, 17 BURP genes belonging to four subfamilies were identified in jujube based on homology analysis, gene structures, and conserved motif confirmation. Gene duplication analysis indicated both tandem duplication and segmental duplication had contributed to ZjBURP expansion. The ZjBURPs were extensively expressed in flowers, young fruits, and jujube leaves. Transcriptomic data and qRT-PCR analysis further revealed that ZjBURPs also significantly influence fruit development, and most genes could be induced by low temperature, salinity, and drought stresses. Notably, several BURP genes significantly altered expression in response to low temperature (ZjPG1) and drought stresses (ZjBNM7, ZjBNM8, and ZjBNM9). Conclusions These results provided insights into the possible roles of ZjBURPs in jujube development and stress response. These findings would help selecting candidate ZjBURP genes for cold- and drought-tolerant jujube breeding.

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BURP domain-containing genes in legumes: genome-wide identification, structure, and expression analysis under stresses and development

Cited by 7 publications

References 48 publications

Comprehensive and evolutionary analysis of Spodoptera litura-inducible Cytochrome P450 monooxygenase gene family in Glycine max elucidate their role in defense

Comprehensive and evolutionary analysis of Spodoptera litura-inducible Cytochrome P450 monooxygenase gene family in Glycine max elucidate their role in defense

Genome-wide characterization and comparative analysis of the OSCA gene family and identification of its potential stress-responsive members in legumes

Identification and expression analysis of BURP domain-containing genes in jujube and their involvement in low temperature and drought response

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