Búsqueda de bacterias marinas como fuente de inhibidores de quorum sensing (iqs): primer estudio químico de Oceanobacillus profundus (RKHC-62B)

Abstract

RESUMENAntecedentes: Los productos naturales aislados de microorganismos marinos han demostrado tener un amplio rango de actividades biológicas incluyendo inhibidores de quorum sensing. Objetivos: Estudiamos quince cepas bacterianas del phylum Firmicutes aisladas del coral blando Antillogorgia elisabethae que fueron evaluadas como una nueva fuente sostenible de compuestos inhibidores de quorum sensing (IQS). Métodos: Se prepararon cultivos en cuatro medios diferentes y extraídos usando una resina no iónica. Todos los extractos fueron probados con el fin de establecer su actividad inhibitoria de quorum sensing (IQS) usando Chromobacterium violaceum ATCC 31532 como biosensor. Los extractos activos fueron fraccionados mediante cartuchos RP-18. Cada fracción fue evaluada mediante bioensayo y analizada por HPLC. Resultados: Las fracciones metanólicas de Jeotgalicoccus halophilus y Oceanobacillus profundus fueron las más activas, lo cual sugiere que los compuestos no polares pueden ser los responsables de esta actividad. Conclusiones: El estudio químico del extracto orgánico de O. profundus cultivado en medio LBS permitió aislar los compuestos tirosol (1) y acetato de tirosol (2) como los responsables de la actividad IQS. Palabras clave: Productos naturales marinos, Microorganismos marinos, Jeotgalicoccus halophilus, Oceanobacillus profundus, Compuestos inhibidores de quorum sensing. ABSTRACTBackground: Natural products isolated from marine microorganisms have demonstrated a wide range of biological activities included quorum sensing inhibitors. Objectives: We study fifteen marine Firmicutes bacterial strains isolated from the Caribbean soft coral Antillogorgia elisabethae that were evaluated as a novel and sustainable source of quorum sensing inhibitor compounds. Methods: Cultures were made in four different culture media and further extracted using a non-ionic resin. All these extracts were tested in order to establish its quorum sensing inhibition (QSI) activity using Chromobacterium violaceum ATCC 31532. The active extracts were fractionated by RP-18 cartridge. Each fraction was tested and evaluated for its composition by HPLC-PDA. Results: Methanol fractions of Jeotgalicoccus halophilus and Oceanobacillus profundus were the most active ones, suggesting that non-polar compounds could be the responsible for the QSI activity of the bacterial extracts here tested. Conclusions: The chemical study

A Quorum-Sensing Inhibitor Strain of Vibrio alginolyticus Blocks Qs-Controlled Phenotypes in Chromobacterium violaceum and Pseudomonas aeruginosa

“…Interestingly, a final concentration of 2 mg/mL of both products was toxic to C. violaceum but not to P. aeruginosa . Other molecules associated with tyramine and N -acetyltyramine, such as N-(2’-phenylethyl)-isobutyramide, 3-methyl-N-(2’-phenylethyl)-butyramide [76], 4-(2-hydroxyethyl)-phenol, and tyrosol acetate [77], have also been found to be capable of inhibiting QS-mediated phenotypes, which corroborates our finding that tyramine and N -acetyltyramine are QS-inhibiting molecules.…”

Fungicidal activity of volatile organic compounds from Paenibacillus bacteria against Colletotrichum gloeosporioides

“…The impact of culture media on bacterial metabolic profiles is well known [19]. To establish the effect of culture media on VOCs composition (qualitative and quantitatively) and their antifungal activity, we studied the impact of four different culture media (MH, NA, ISP2, and LB) on mycelia growth inhibition (Table 2).…”

Widespread Existence of Quorum Sensing Inhibitors in Marine Bacteria: Potential Drugs to Combat Pathogens with Novel Strategies

“…Screening from various marine environments using either biosensor strains or metagenomic sequencing showed abundance of QS-inhibitory marine bacteria (Figure 1) [30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49]. It could be seen that QS-inhibitory marine bacteria were mainly screened out from sea waters, marine sediments, as well as marine invertebrates, fish, algae, etc.…”

“…Based on screening, many researches have isolated one or several QS-inhibitory bacteria strains from marine origins. The identified bacteria, which have potential QS inhibition ability but have not been further explored for specific QSIs, were categorized in Figure 2 [33,34,35,37,38,40,41,42,43,44,46,47,48,49,50,51,52,53,54,55]. Statistically, QS-inhibitory bacteria could be divided into four phylums and five classes.…”