C/D-Box Snord105b Promotes Tumorigenesis in Gastric Cancer via ALDOA/C-Myc Pathway

Abstract

Background/Aims: Small nucleolar RNAs (snoRNAs) play an important role in carcinogenesis. In this study, we identified a C/D box snoRNA, snord105b, and further investigated the function and mechanism of the snord105b in gastric cancer (GC). Methods: The expression level of snord105b in GC tissures, sera and cell lines were detected by qRT-PCR. Cell viability was assessed using MTS assay. Transwell and wound healing assay were performed to evaluate migration and invasion, and protein expression was examined by western blotting. ChIRP and MS analysis was used to seek for the special binding protein of snord105b. Results: The snord105b was upregulated and associated with tumor size, differentiation, and pathological stage in GC. Snord105b affected proliferation, migration and invasion in multiple GC cell lines. The oncoqenic activity of snord105b was also confirmed with in vivo data. Mechanistically, snord105b specifically bound to ALDOA and affected C-myc, which plays a key role in carcinogenesis and tumor development. Conclusion: Snord105b appears to be a novel oncogene and is clinically and functionally involved in the development of GC. Targeting snord105b and its pathway may provide new biomarkers or potential treatments for patients with GC.

Loss‐of‐Function Genetic Screening Identifies Aldolase A as an Essential Driver for Liver Cancer Cell Growth Under Hypoxia

“…( 25 ) Previous studies have found that ALDOA was highly expressed in lung cancer, pancreatic cancer, and gastric cancer. ( 37 , 38 , 39 ) Hu et al showed that the activity of ALDOA was increased under stimulation of phosphoinositide 3‐kinase–dependent activation of Rac by releasing filamentous actin‐bound ALDOA, linking actin remodeling to glycolysis. ( 40 ) Overexpression of ALDOA promoted formation of a cancer‐associated proton pump inhibitor with γ‐actin and accelerated lung cancer metastasis, ( 37 ) suggesting its role as an oncogene in tumor progression.…”

Small Nucleolar RNA 71A Promotes Lung Cancer Cell Proliferation, Migration and Invasion via MAPK/ERK Pathway

“…snoRNA ACA11 was reported to promote human hepatoma cell line Huh7 proliferation and EMT through the PI3K/AKT pathway 12. SNORD105b was overexpressed in gastric cancer tissues and induced tumorigenesis via ALDOA/C-Myc pathway 13. SNORA23 promoted pancreatic ductal adenocarcinoma cell proliferation and invasion, as well as tumor growth and metastasis, via inducing spectrin repeat-containing nuclear envelope 2 in mice 14.…”

Emerging Data on the Diversity of Molecular Mechanisms Involving C/D snoRNAs

“…This is especially prominent in cancer as biopsies typically show altered expression of subsets of snoRNA genes [173][174][175] (for a review, see [176]) and have suggested their potential as diagnostic or prognostic markers [176]. Hence, several snoRNAs have been proposed as proto-oncogenes or tumor suppressors based on in vitro analyses [177][178][179][180][181]. They are also actors of other conditions, e.g., the Prader Willi syndrome (PWS) for the snord116 snoRNAs (for a review, see [182]), or the leukoencephalopathy with brain calcifications and cysts (LCC) for the U8 snoRNA [183].…”