C1QTNF1-AS1 regulates the occurrence and development of hepatocellular carcinoma by regulating miR-221-3p/SOCS3

Li, Hang; Zhang, Bo; Ding, Meng; Lü, Shaowu; Zhou, Hui; Sun, Donglin; Wu, Gang; Gan, Xianfeng

doi:10.1007/s12072-019-09944-5

Cited by 28 publications

(37 citation statements)

References 26 publications

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“…The overexpression of tumor suppressor miRNAs inhibits cellular proliferation, invasion, migration, metastasis, and angiogenesis by the downregulation of AEG-1 in HCC [8,27,28,29] and cervical cancer cells [11]. On the other hand, overexpression of miR-221 promotes cell proliferation, metastasis by the dysregulation of tumor suppressors and oncogenes in HCC [24,25,26]. Moreover, miR-221 regulates angiogenesis and knockdown of miR-221 inhibits SND1-mediated angiogenesis activity in HCC cells [35].…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies showed miR-221 is overexpressed and regulated as an individual or cluster in HCC [25,26]. On the other hand, AEG-1 is regulated by multiple miRNAs during carcinogenesis of HCC [25,27].…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, AEG-1 is regulated by multiple miRNAs during carcinogenesis of HCC [25,27]. Moreover, few studies have investigated the regulative network axis, particularly miR-221 target genes (PHF2 [25], C1QTNF1-AS1 [26], and miRNAs (miR-375 [27,28] and miR-195 [29]) targeting AEG-1 in HCC. Since the studies on the miR-221/AEG-1 axis on target/associate genes in HCC are still limited and unclear, we aimed to analyze the molecular mechanism of the miR-221/AEG-1 axis and possible targets in HCC.…”

Section: Introductionmentioning

confidence: 99%

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RETRACTED: AEG-1/miR-221 Axis Cooperatively Regulates the Progression of Hepatocellular Carcinoma by Targeting PTEN/PI3K/AKT Signaling Pathway

Kannan

Jayamohan

Moorthy

et al. 2019

IJMS

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Hepatocellular carcinoma (HCC) is the third leading malignancy worldwide, causing mortality in children and adults. AEG-1 is functioned as a scaffold protein for the proper assembly of RNA-induced silencing complex (RISC) to optimize or increase its activity. The increased activity of oncogenic miRNAs leads to the degradation of target tumor suppressor genes. miR-221 is an oncogenic miRNA, that plays a seminal role in carcinogenesis regulation of HCC. However, the molecular mechanism and biological functions of the miR-221/AEG-1 axis have not been investigated extensively in HCC. Here, the expression of miR-221/AEG-1 and their target/associate genes was analyzed by qRT-PCR and Western blot. The role of the miR-221/AEG-1 axis in HCC was evaluated by proliferation assay, migration assay, invasion assay, and flow cytometry analysis. The expression level of miR-221 decreased in AEG-1 siRNA transfected HCC cells. On the other hand, there were no significant expression changes of AEG-1 in miR-221 mimic and miR-221 inhibitor transfected HCC cells and inhibition of miR-221/AEG-1 axis decreased cell proliferation, invasion, migration, and angiogenesis and induced apoptosis, cell cycle arrest by upregulating p57, p53, PTEN, and RB and downregulating LSF, MMP9, OPN, Bcl-2, PI3K, AKT, and LC3A in HCC cells. Furthermore, these findings suggest that the miR-221/AEG-1 axis plays a seminal oncogenic role by modulating PTEN/PI3K/AKT signaling pathway in HCC. In conclusion, the miR-221/AEG-1 axis may serve as a potential target for therapeutics, diagnostics, and prognostics of HCC.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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RETRACTED: AEG-1/miR-221 Axis Cooperatively Regulates the Progression of Hepatocellular Carcinoma by Targeting PTEN/PI3K/AKT Signaling Pathway

Kannan

Jayamohan

Moorthy

et al. 2019

IJMS

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“…The lncRNA C1QTNF1 antisense RNA 1 (C1QTNF1-AS1) has been reported to contribute to the tumorigenesis of hepatocellular carcinoma. 28,29 However, its expression and detailed functions in CRC are not well understood. This study aimed to determine C1QTNF1-AS1expression in CRC, investigate the important roles played by C1QTNF1-AS1 in CRC cells, and elucidate the underlying molecular events.…”

Section: Introductionmentioning

confidence: 99%

<p>Long Non-Coding RNA C1QTNF1 Antisense RNA 1 Upregulates Hexokinase 2 by Sponging microRNA-484 to Promote the Malignancy of Colorectal Cancer</p>

Jin¹,

Liu²,

Wang³

et al. 2020

CMAR

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The long noncoding RNA C1QTNF1 antisense RNA 1 (C1QTNF1-AS1) contributes to hepatocellular carcinoma development. However, its expression and roles in colorectal cancer (CRC) have not been fully explored. Therefore, this study determined the expression and roles of C1QTNF1-AS1 in CRC and elucidated its detailed mechanism of action. Methods: C1QTNF1-AS1 expression in CRC tissues and cell lines was assessed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). We used Cell Counting Kit-8, flow cytometry, cell migration and invasion assays, and a xenograft tumor model to test the effects of C1QTNF1-AS1 on CRC malignancy. The associations among C1QTNF1-AS1, microRNA-484 (miR-484), and hexokinase 2 (HK2) were explored using luciferase reporter assay, RNA immunoprecipitation, RT-qPCR, and Western blotting. Results: C1QTNF1-AS1 was overexpressed in CRC and related to poor prognosis. C1QTNF1-AS1 interference inhibited CRC cell proliferation, migration, and invasion but induced apoptosis. Furthermore, C1QTNF1-AS1 deficiency impaired tumor growth in vivo. Mechanistically, C1QTNF1-AS1 adsorbed miR-484, thereby increasing the expression of its target HK2. Rescue experiments revealed that the effects of C1QTNF1-AS1 deficiency in CRC cells were reversed by inhibiting miR-484 or upregulating HK2. Conclusion: C1QTNF1-AS1 drives CRC progression by sponging miR-484 and consequently upregulating HK2. The C1QTNF1-AS1/miR-484/HK2 pathway may serve as a diagnostic and therapeutic target for CRC.

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“…Hence, this study focused attention on the BMMSC MVs-derived miR-221-3p. Enormous studies have revealed that miR-221-3p is aberrantly expressed in various cancers and participate in the regulation of tumorigenesis and development, like cervical squamous carcinoma (Wu et al, 2019), hepatocellular carcinoma (Li et al, 2019), medulloblastoma (Yang et al, 2019), and breast cancer (Ergun et al, 2015). However, the role of miR-221-3p in AML has not been reported.…”

Section: Discussionmentioning

confidence: 99%

miR-221-3p Delivered by BMMSC-Derived Microvesicles Promotes the Development of Acute Myelocytic Leukemia

Zhang

Wang

et al. 2020

Front. Bioeng. Biotechnol.

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The study aims to investigate the effects of miR-221-3p in bone marrow mesenchymal stem cell (BMMSC)-derived microvesicles (MVs) on cell cycle, proliferation and invasion of acute myelocytic leukemia (AML). Methods: Bioinformatics was used to predict differentially expressed miRNAs (DEmiRNAs) in AML. The morphology of BMMSC-derived MVs was observed under an electron microscope, and the positional relation of MVs and OCI-AML2 cells was observed by a fluorescence microscope. MTT, Transwell, and flow cytometry assays were used to analyze the effects of MVs on OCI-AML2 cells. The targeted relationship between miR-221-3p and CDKN1C was detected by dual luciferase assay. Results: It was verified that miR-221-3p promoted the proliferation, invasion and migration of OCI-AML2 cells, and induced the cell cycle arrest in G1/S phase as well as inhibited cell apoptosis. Further studies showed that MVs promoted the proliferation, migration and invasion of AML, and induced the cell cycle arrest in G1/S phase through miR-221-3p. It was confirmed that miR-221-3p can directly target CDKN1C to regulate cell cycle, proliferation and invasion of AML. Conclusion: miR-221-3p in BMMSC-derived MVs regulated AML cell cycle, cell proliferation and invasion through targeting CDKN1C. miR-221-3p and CDKN1C were considered to be potential targets and biomarkers for the treatment of AML in clinic.

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C1QTNF1-AS1 regulates the occurrence and development of hepatocellular carcinoma by regulating miR-221-3p/SOCS3

Cited by 28 publications

References 26 publications

RETRACTED: AEG-1/miR-221 Axis Cooperatively Regulates the Progression of Hepatocellular Carcinoma by Targeting PTEN/PI3K/AKT Signaling Pathway

RETRACTED: AEG-1/miR-221 Axis Cooperatively Regulates the Progression of Hepatocellular Carcinoma by Targeting PTEN/PI3K/AKT Signaling Pathway

<p>Long Non-Coding RNA C1QTNF1 Antisense RNA 1 Upregulates Hexokinase 2 by Sponging microRNA-484 to Promote the Malignancy of Colorectal Cancer</p>

miR-221-3p Delivered by BMMSC-Derived Microvesicles Promotes the Development of Acute Myelocytic Leukemia

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