Ca²⁺-Activated Reactive Oxygen Species Production by Arabidopsis RbohH and RbohJ Is Essential for Proper Pollen Tube Tip Growth

Role of Reactive Oxygen Species during Cell Expansion in Leaves

“…If NADPH oxidase-derived O 2 2 is required for cell wall stiffening by H 2 O 2 , then loss-of-function mutants would be expected to develop more elongated cells. In contrast, mutations in NADPH oxidases have been shown to impair the elongation of root hairs and pollen tubes (Foreman et al, 2003;Takeda et al, 2008;Kaya et al, 2014) and the growth of leaves (Chaouch et al, 2012). Thus, NADPH oxidases are positive regulators of cell growth.…”

“…ROS, like hydrogen peroxide (H 2 O 2 ), hydroxyl radicals ( _ OH), and superoxide radicals (O 2 2 ), have been implicated in developmental processes and emerged as key signaling molecules in plants (Schmidt and Schippers, 2015). For instance, during root hair and pollen elongation, ROS play fundamental roles in the spatial regulation of polar cell growth (Takeda et al, 2008;Kaya et al, 2014). Still, the role of ROS in the regulation of leaf development remains unclear.…”

A Cotton Annexin Affects Fiber Elongation and Secondary Cell Wall Biosynthesis Associated with Ca2+ Influx, ROS Homeostasis, and Actin Filament Reorganization

“…S5, A and B). It has been shown that Ca 2+ -activated ROS production by RbohH and RbohJ is essential for proper pollen tube tip growth (Kaya et al, 2014). We investigated whether ROS produced by Rboh contributes to fiber cell development.…”

Apoplastic ROS production upon pollination by RbohH and RbohJ in Arabidopsis

“…[2][3][4] Apoplastic ROS are suggested to play a role in cell wall loosening in maize coleoptiles, leaves, and roots.…”

“…1 Reactive oxygen species (ROS) accumulate at the tip of growing pollen tubes and 2 NADPH oxidases, respiratory burst oxidase homolog H (RbohH) and RbohJ, specifically localized in the plasma membrane of the growing tip 2 are involved in this accumulation. [2][3][4] The ROS are suggested to stimulate the plasma membrane Ca 2C -permeable channel(s) and induce Ca 2C influx into the cytoplasm. 5 The cytosolic Ca 2C rise in turn activates the ROS-producing activity of Rboh proteins by direct binding to the EF-hand motifs and by phosphorylation through the Ca 2C -dependent protein kinases, generating a positive feedback regulation that maintains the growth site to the tip during elongation (Fig.…”