Ca2+ Movements in Smooth Muscle

Breemen, Cornelis van; Aaronson, Philip I.; Loutzenhiser, Rodger; Meisheri, Kaushik D.

doi:10.1378/chest.78.1.157

Cited by 39 publications

(26 citation statements)

References 29 publications

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“…The mode of action most favoured by recent workers is that ethanol alters the level of intracellular calcium at the contractile filaments (Hurwitz etal., 1962;Kalsner, 1970;Seeman, 1972;Ross, 1976;Turlapaty et al, 1979a;Mayer, Khanna & Kalant, 1980). Calcium for smooth muscle contraction is derived from one of three sources, the extracellular fluid, a small labile store loosely bound to the external membrane and an intracellular store associated with endoplasmic reticulum and, perhaps, mitochondria and the inner surface of the plasma membrane (van Breemen, Aaronson, Loutzenhiser & Meisheri, 1980). Muscle contraction is ended by sequestering calcium within these organelles followed by a slower extrusion from the cell (Vallieres, Scarpa & Somlyo, 1975;van Breemen et al, 1980).…”

Section: Discussionmentioning

confidence: 99%

“…Calcium for smooth muscle contraction is derived from one of three sources, the extracellular fluid, a small labile store loosely bound to the external membrane and an intracellular store associated with endoplasmic reticulum and, perhaps, mitochondria and the inner surface of the plasma membrane (van Breemen, Aaronson, Loutzenhiser & Meisheri, 1980). Muscle contraction is ended by sequestering calcium within these organelles followed by a slower extrusion from the cell (Vallieres, Scarpa & Somlyo, 1975;van Breemen et al, 1980). With this background we would like to propose a mode of action for ethanol which makes two assumptions.…”

Section: Discussionmentioning

confidence: 99%

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The Effect of Ethanol on Inhibitory and Motor Responses in the Rat and Rabbit Anococcygeus and the Bovine Retractor Penis Muscles

Gillespie

Hunter

McKnight

1982

British J Pharmacology

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Ethanol (200 mm) reduced the response to inhibitory nerve stimulation in the rat and rabbit anococcygeus and the bovine retractor penis (BRP) muscles. Ethanol also reduced the response to the inhibitory extract from the BRP consistent with the inhibitory factor in these extracts playing some part in the response to inhibitory nerve stimulation. Ethanol's effect on the response to other inhibitory stimuli was examined in the rabbit anococcygeus and the BRP. In the anococcygeus the response to carbachol was reduced, to bradykinin and isobutylmethylxanthine (IBMX) unaltered, and to isoprenaline and adenosine 5′‐triphosphate (ATP) potentiated. In the BRP responses to IBMX and sodium nitroprusside were unaltered but in this tissue the response to isoprenaline was reduced. Ethanol's ability to reduce inhibitory responses is, therefore, selective and confined to inhibitory nerve stimulation, inhibitory extract, carbachol and, in the BRP, isoprenaline. Ethanol reduced the rate of development of inhibition even where the magnitude of the inhibitory response was unaltered. In the rat anococcygeus, ethanol (200 mm) potentiated the response to motor nerve stimulation and to noradrenaline (NA) at low frequencies and low concentrations respectively. Higher ethanol concentrations (400 mm) reduced the response to both motor nerve stimulation and NA. The motor response to carbachol was also reduced. Ethanol (200 mm) itself caused an easily reversible contraction in all three tissues. This was not due to the release of NA but was highly sensitive to the removal of external calcium from the medium. A unified explanation of these varied effects of ethanol based on a reduction in membrane binding of calcium and a reduced efficiency of receptor coupling is suggested.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Effect of Ethanol on Inhibitory and Motor Responses in the Rat and Rabbit Anococcygeus and the Bovine Retractor Penis Muscles

Gillespie

Hunter

McKnight

1982

British J Pharmacology

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“…7 Cytoplasmic calcium levels in vascular smooth muscle are regulated by calcium channel activity, membrane-bound calcium pumps, and intracellular sequestering mechanisms. 21 Since the level of cytosolic calcium directly determines the extent of force generated by vascular smooth muscle, 22 alteration in agonist-induced calcium mobilization, specifically, a decrease in calcium influx. The present study was designed to test this hypothesis.…”

Section: " 620mentioning

confidence: 99%

Simultaneous measurement of force and calcium uptake during acetylcholine-induced endothelium-dependent relaxation of rabbit thoracic aorta.

Ratz¹,

Gleason

Flaim³

1987

Circulation Research

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This study was designed to determine whether the endothelium-derived relaxing factor induced by acetylcholine (1 microM) in rabbit thoracic aorta inhibits agonist-induced calcium mobilization, specifically calcium influx. Force generated in rings of rabbit thoracic aorta by norepinephrine (1 microM) was measured under isometric conditions. At the appropriate time during 1 microM acetylcholine-induced relaxation of 1 microM norepinephrine-contracted rabbit thoracic aorta, the rings were pulse-labelled with calcium-45 to measure calcium influx. When measured in this fashion, 1 microM acetylcholine decreased the 1 microM norepinephrine-induced increase in calcium influx. This effect was eliminated by removal of the endothelium and by atropine (1 microM), but not by indomethacin (14 microM). Acetylcholine (1 microM) also blocked the 60 mM potassium-chloride-induced increase in calcium influx without dramatically affecting force. The phasic contraction produced by norepinephrine (1 microM) with 2 mM lanthanum pretreatment, which is caused by release of intracellular calcium, was inhibited by acetylcholine (1 microM) in a fashion similar to 1 microM nitroglycerin. The tonic contraction produced by norepinephrine (1 microM) after depletion of the agonist-releasable pool of intracellular calcium, which is thought to be due to calcium influx, was depressed by acetylcholine (1 microM). These data suggest that endothelium-derived relaxing factor relaxes 1 microM norepinephrine-contracted rings of rabbit thoracic aorta by decreasing calcium entry and by producing an extracellular calcium-independent relaxant effect.

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“…This idea is feasible since the minimum concentration of Ca required to produce the contraction in different vascular smooth muscle in skinned conditions was in order of 10-7 M-free Ca (Itoh, Kuriyama & Suzuki, 1981). The functional relationships between the myoplasmic membrane and sarcoplasmic membrane were discussed in relation to direct coupling between the activation of the myoplasmic membrane and release of stored Ca in the cell (Brading, 1979; Gabella, 1979;Brading, Burnett & Sneddon, 1980;Johansson & Somlyo, 1980;Van Breemen, Aaronson, Loutzenhiser & Meisheri, 1980;.…”

Section: Introductionmentioning

confidence: 99%

Roles of stored calcium on the mechanical response evoked in smooth muscle cells of the porcine coronary artery

Itoh

Kajiwara

Kitamura

et al. 1982

The Journal of Physiology

119

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SUMMARYEffects of acetylcholine (ACh), caffeine and procaine on the membrane and mechanical properties of the intact and skinned muscle cells of the porcine coronary artery were investigated using the micro-electrode and isometric tension recording methods.1. ACh (10-8-10-5 M) had no effect on the membrane potential and membrane resistance, as assessed from the current-voltage relationship.2. Caffeine possessed dual actions on the membrane property, i.e. a low concentration (0 3-1 mM) hyperpolarized and a high concentration (over 1 mM) depolarized the membrane, while caffeine (over 0 3 mM) consistently increased the ionic conductance of the membrane. Procaine (over 1 mM) depolarized the membrane and decreased the ionic conductance of the membrane.3. The spike and contraction evoked by outward current pulses in the presence of 10 mM-TEA were suppressed by treatment with caffeine (5 mM) or procaine (3 mM), but the spike was slightly and the electrically induced contraction was significantly suppressed by ACh (10-6 M) due to the marked increase in resting tension.4. In Ca-free 2 mM-EGTA containing solution, the K induced contraction (118 mM) rapidly ceased, but not the contraction evoked by ACh (10-5 M) or caffeine (5 mM). The ACh-induced contraction rapidly ceased in the presence of 1 mMprocaine; however, over 5 mM-procaine was required to abolish the caffeine-induced contraction.5. The pCa-tension relationship was measured in saponin-treated skinned muscles. The minimum concentration offree Ca required to produce contraction was 2 x 1O-' M, while maximum contraction was observed at 10-5 M-free Ca. The maximum amplitude of Ca-induced contraction observed in skinned muscles was larger or the same as that evoked by ACh in the intact muscle. ACh (10-M), caffeine (5 mM) and procaine (5 mM) had no effect on the pCa-tension relationship.6. After Ca has been loaded in skinned muscles, caffeine and replacement of K with choline (116 mM) in the Ca-free EGTA containing solution produced the contraction. However, the application of ACh did not result in a release of the stored Ca, and procaine suppressed the release of Ca activated by caffeine. 108T. ITOH AND OTHERS 7. The present results indicate that ACh activates the muscarinic receptor distributed on the plasma membrane, thus releasing the stored Ca. However, this mechanism may not be a prerequisite for direct action of ACh on the Ca releasing site. The possible mechanism of ACh action on the Ca releasing site, mainly sarcoplasmic reticulum, was discussed in relation to actions of caffeine and procaine, particularly with regard to the functional relations between plasma membrane and sarcoplasmic reticulum.

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Ca2+ Movements in Smooth Muscle

Cited by 39 publications

References 29 publications

The Effect of Ethanol on Inhibitory and Motor Responses in the Rat and Rabbit Anococcygeus and the Bovine Retractor Penis Muscles

The Effect of Ethanol on Inhibitory and Motor Responses in the Rat and Rabbit Anococcygeus and the Bovine Retractor Penis Muscles

Simultaneous measurement of force and calcium uptake during acetylcholine-induced endothelium-dependent relaxation of rabbit thoracic aorta.

Roles of stored calcium on the mechanical response evoked in smooth muscle cells of the porcine coronary artery

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