Caesalpinia sappan L. heartwood ethanolic extract exerts genotoxic inhibitory and cytotoxic effects

Meiyanto, Edy; Lestari, Beni; Sugiyanto, Raisatun Nisa; Jenie, Riris Istighfari; Utomo, Rohmad Yudi; Sasmito, Ediati; Murwanti, Retno

doi:10.1007/s13596-018-0351-9

Cited by 8 publications

(8 citation statements)

References 33 publications

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“…(29) However, Dox is a chemotherapeutic agent relatively inexpensive, which is still expected to be used to cure cancer. Be can be easily obtained because of its abundant availability in Sappan wood (10,30,31); hence, it can be used as complementary medicine to overcome Dox's problem.…”

Section: Discussionmentioning

confidence: 99%

Co-treatment of Brazilein Enhances Cytotoxicity of Doxorubicin on WiDr Colorectal Cancer Cells Through Cell Cycle Arrest

et al. 2020

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BACKGROUND: The presence of adverse side effects limits the use of doxorubicin (Dox) despite its cost-effectiveness compared to other chemotherapeutic agents. Brazilein (Be), the major compound of Caesalpinia sappan, performs co-chemotherapeutic potency in several cancer cell lines. This study evaluates the chemosensitizing effects of Be to Dox on colon cancer cell line, WiDr.METHODS: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was conducted to evaluate the cytotoxic effect of Be and its combination with Dox. The synergistic effect of Be and Dox was examined by using the Combination index (CI) parameter. Cell cycle and apoptosis profiles were done using flow cytometry with propidium iodide (PI)/RNase and Annexin V staining, respectively.RESULTS: The combination of Dox and Be at half of IC50 on WiDr cells showed a synergistic effect with a combination index of 0.4. Analysis of the cell cycle revealed that the combination caused cell cycle termination at the S and G2/M phase. This finding corresponded with the data that single treatment of Dox and Be induced cell cycle arrest at the different phases, namely S and G2/M phase, respectively. However, the combination treatment for 24 hours did not induce apoptosis. This combination should be further clarified as there was a possibility that many cells may underwent permanently arrest that halts to proceed apoptosis.CONCLUSION: Our findings suggested that Be synergizes with Dox to suppress the growth of WiDr cells via cell cycle arrest, hence, Be is potential to be developed as a co-chemotherapeutic agent. Our findings suggested that Be synergizes with Dox to suppress the growth of WiDr cells via cell cycle arrest, hence, Be is potential to be developed as a co-chemotherapeutic agent.KEYWORDS: Brazilein, colon cancer WiDr, co-treatment, Doxorubicin, cell cycle arrest

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Section: Discussionmentioning

confidence: 99%

Co-treatment of Brazilein Enhances Cytotoxicity of Doxorubicin on WiDr Colorectal Cancer Cells Through Cell Cycle Arrest

et al. 2020

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“…Apoptosis results in fragmentation of the cell nucleus genome. It has a vital role in eradicating cancer cells and is an essential target in discovering anti-cancer drugs (Meiyanto et al, 2019). To clarify whether the cell cycle arrest effect under VO treatments is correlated with apoptosis phenomena, we then carried out flow cytometry analysis with PI staining on VO treated cells.…”

Section: Apoptosis Effect Of Vomentioning

confidence: 99%

Different Cytotoxic Effects of Vetiver Oil on Three Types of Cancer Cells, Mainly Targeting CNR2 on TNBC

Hanifa

Wulandari

Zulfin

et al. 2022

Asian Pac J Cancer Prev

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prostate, uterus, and vascular endothelium. Therefore, this receptor is dominant in cognitive function, memory, anxiety, pain, motor regulation, and endocrine regulation (Dhopeshwarkar and Mackie, 2014). Meanwhile, high expression of CNR2 is found in the immune system cells, but it is also functionally expressed in the brain (Feng et al., 2015;Elbaz et al., 2017). The different tissue distributions of CNR1 and CNR2 allow different receptor effects with selective and specific activation pathways. Both types of CNR are highly expressed in various cancer tissues, but CNR2 plays a more critical role in carcinogenesis and

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“…The heartwood of sappan powder was obtained from the Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional (B2P2TOOT), Tawangmangu, In donesia, and was determined in the Faculty of Phar macy, Universitas Gadjah Mada. The extraction of heart wood was performed according to Meiyanto et al (2019).…”

Section: Sample Preparationmentioning

confidence: 99%

Ethanolic extract of sappan wood (Caesalpinia sappan L.) inhibits MCF-7 and MCF-7/HER2 mammospheres' formation: an in vitro and bioinformatic study

Novitasari

Muntafiah

Sari

et al. 2021

Indones. J. Biotechnol.

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One of the mechanisms of cancer cell resistance toward chemotherapy is through cancer stem cells (CSCs), which are characterized by excessive activation of regulator proteins such as human epidermal receptor 2 (HER2). Sappan wood (Caesalpinia sappan L.) contains brazilin and brazilein that exhibit cytotoxic effects on several cancer cell lines. We aimed to explore the potency of the ethanolic extract of sappan (EES) in CSCs through bioinformatic analyses and by using a three-dimensional (3D) breast cancer stem cells (BCSCs) for in vitro assay with two different models (i.e., BCSCs and HER2-BCSCs) in order to identify the potential therapeutic targets of genes (PTTGs). Bioinformatic analyses identiﬁed PTTGs, which were further analyzed by gene ontology, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, protein-protein interaction (PPI) networks, and hub protein selection. Mammospheres were cultured under conditioned media. The cytotoxic effects of EES were then measured by direct counting and based on the mammosphere-forming potential (MFP). Bioinformatic analysis disclosed PIK3CA and TP53 as PTTGs in BCSCs and HER2-BCSCs, respectively. In addition, the KEGG pathway analyses also demonstrated that PTTGs could regulate the ERBB pathway. EES thus demonstrated cytotoxicity and inhibited the formation of mammospheres. Collectively, EES exhibited excellent potential for further development as an inhibitor of cancer stem cells in breast cancer.

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Caesalpinia sappan L. heartwood ethanolic extract exerts genotoxic inhibitory and cytotoxic effects

Cited by 8 publications

References 33 publications

Co-treatment of Brazilein Enhances Cytotoxicity of Doxorubicin on WiDr Colorectal Cancer Cells Through Cell Cycle Arrest

Co-treatment of Brazilein Enhances Cytotoxicity of Doxorubicin on WiDr Colorectal Cancer Cells Through Cell Cycle Arrest

Different Cytotoxic Effects of Vetiver Oil on Three Types of Cancer Cells, Mainly Targeting CNR2 on TNBC

Ethanolic extract of sappan wood (Caesalpinia sappan L.) inhibits MCF-7 and MCF-7/HER2 mammospheres' formation: an in vitro and bioinformatic study

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