Calbindin-D28k-immunoreactive cells and fibres in the human amygdaloid complex

Sorvari, Heikki; Soininen, Hilkka; Pitkänen, Asla

doi:10.1016/0306-4522(96)00296-5

Cited by 48 publications

(51 citation statements)

References 67 publications

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“…These results are in apparent contradiction with a dual-antigen immunocytochemistry study in the rodent amygdala by McDonald and Betette (2001), reporting that 80% of PVB-IR neurons in the amygdala were found to coexpress CB. On the other hand, this estimate is consistent with reports describing the distribution of CB-and PVB-IR neurons in the human amygdala as complementary rather than overlapping (Sorvari et al, 1995(Sorvari et al, ,1996b. On a speculative level, such a contradiction may be explained by evidence suggesting an intriguing trend toward a decrease of PVB-CB coexpression in primate cortical or cortex-like regions compared with equivalent brain regions in rodents.…”

supporting

confidence: 79%

“…Such an occurrence might have had a impact on the proportion of neuronal subgroups reported in this paper. For instance, the numbers of CB-IR neurons counted in the five subjects included in this study appear to be lower than those reported for one subject by Sorvari et al (1996b). The reasons for this discrepancy are not clear at this time and may be multifold.…”

Section: Coexpression Of Pvb and Cb In Human Amygdala Neuronscontrasting

confidence: 46%

“…In rodents, approximately 80% of all amygdalar PVB-IR neurons also express CB (McDonald and Betette, 2001). Investigations in monkeys and humans suggest a more moderate overlap (Hartig et al, 1995;Sorvari et al, 1996b;Pitkanen and Kemppainen, 2002). Colocalization of PVB and CB in the human amygdala has been examined in the present study for the first time.…”

Section: Nih-pa Author Manuscriptmentioning

confidence: 77%

“…Similar to PVB-IR neurons, CB-IR cells have also been shown to be morphologically heterogeneous, with small and large multipolar neurons representing the most numerous groups (Sorvari et al, 1996b). In this study, all neurons displaying IR for CB, but not the other two markers, were grouped under the general category of "multipolar" because of the technical limitations relative to the immunofluorescent labeling, which did not allow us to visualize clearly the thin dendrites that characterize these neurons on the basis of the CB-IR alone (Sorvari et al, 1996b). Particularly large neurons with more intense dendritic IR were labeled as LM (Fig.…”

Section: Cb-ir Neuronsmentioning

confidence: 99%

“…2L). Light microscopy analysis showed that the distribution patterns of PVB, CB, and PNNs staining in our tissue correspond well to those detected in the human and monkey amygdala and entorhinal cortex in other laboratories (Hartig et al, 1995;Sorvari et al, 1995Sorvari et al, ,1996bMikkonen et al, 1997). Standard tests for antisera specificity included omission of either the primary or the secondary antisera.…”

mentioning

confidence: 98%

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Subpopulations of neurons expressing parvalbumin in the human amygdala

Pantazopoulos

Lange

Hassinger

et al. 2006

J of Comparative Neurology

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Amygdalar intrinsic inhibitory networks comprise several subpopulations of γ-aminobutyric acidergic neurons, each characterized by distinct morphological features and clusters of functionally relevant neurochemical markers. In rodents, the calcium-binding proteins parvalbumin (PVB) and calbindin D28k (CB) are coexpressed in large subpopulations of amygdalar interneurons. PVBimmunoreactive (-IR) neurons have also been shown to be ensheathed by perineuronal nets (PNN), extracellular matrix envelopes believed to affect ionic homeostasis and synaptic plasticity. We tested the hypothesis that differential expression of these three markers may define distinct neuronal subpopulations within the human amygdala. Toward this end, triple-fluorescent labeling using antisera raised against PVB and CB as well as biotinylated Wisteria floribunda lectin for detection of PNN was combined with confocal microscopy. Among the 1,779 PVB-IR neurons counted, 18% also expressed CB, 31% were ensheathed in PNN, and 7% expressed both CB and PNN. Forty-four percent of PVB-IR neurons did not colocalize with either CB or PNN. The distribution of each of these neuronal subgroups showed substantial rostrocaudal gradients. Furthermore, distinct morphological features were found to characterize each neuronal subgroup. In particular, significant differences relative to the distribution and morphology were detected between PVB-IR neurons expressing CB and PVB-IR neurons wrapped in PNNs. These results indicate that amygdalar PVB-IR neurons can be subdivided into at least four different subgroups, each characterized by a specific neurochemical profile, morphological characteristics, and three-dimensional distribution. Such properties suggest that each of these neuronal subpopulations may play a specific role within the intrinsic circuitry of the amygdala. Keywordscalbindin D28k; perineuronal nets; immunocytochemistry; confocal microscopy; basolateral complex of the amygdala The basolateral complex of the amygdala (lateral, basolateral, and accessory basal nuclei; BLC) plays an important role in assigning affective and motivational significance to sensory inputs, forming emotional memories, and generating emotional responses (Gloor, 1986;Halgren, 1992;Brothers, 1990;LeDoux, 1992;Aggleton, 1993;Adolphs et al., 1994). Complex intrinsic inhibitory circuits are believed to represent a critical component of the neural networks NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript underlying these functions. Among the several subpopulations of interneurons forming these circuits, those expressing the calcium-binding protein parvalbumin (PVB) are localized preferentially within the BLC (Sorvari et al., 1995). These neurons have been shown to affect information processing in this region crucially. For instance, the extremely low spontaneous firing rates typical of BLC projection neurons are thought to be the result of strong intrinsic inhibitory pressures originating from PVB-immunoreactive (-IR) neurons (Rainnie et al., 1991;Par...

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supporting

confidence: 79%

Section: Coexpression Of Pvb and Cb In Human Amygdala Neuronscontrasting

confidence: 46%

Section: Nih-pa Author Manuscriptmentioning

confidence: 77%

Section: Cb-ir Neuronsmentioning

confidence: 99%

mentioning

confidence: 98%

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Subpopulations of neurons expressing parvalbumin in the human amygdala

Pantazopoulos

Lange

Hassinger

et al. 2006

J of Comparative Neurology

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Distribution of parvalbumin-, calretinin-, and calbindin-D28k-immunoreactive neurons and fibers in the human entorhinal cortex

1997

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Parvalbumin, calretinin, and calbindin-D28k are calcium-binding proteins that are located in largely nonoverlapping neuronal populations in the brain. The authors studied the distribution of parvalbumin-, calretinin-, and calbindin-D28k-immunoreactive (ir) cells, fibers, terminals, and neuropil in the eight subfields of the human entorhinal cortex. The distribution of each of the three calcium-binding proteins largely followed the cytoarchitectonic borders of the eight entorhinal subfields, although the regional and laminar distributions of the three proteins were segregated rather than overlapping. The highest density of parvalbumin-ir neurons and terminals was found in the caudal and lateral subfields of the entorhinal cortex. Calretinin and calbindin-D28k immunoreactivities were high rostromedially, although a large number of calretinin and calbindin-D28k neurons were also found in the caudal subfields. All parvalbumin-ir cells had a morphological appearance of nonpyramidal neurons. Parvalbumin-ir terminals formed basket-like formations around unstained somata and cartridges, suggesting that parvalbumin neurons compose a subpopulation of gamma-aminobutyric acid (GABA)ergic basket cells and chandelier cells, respectively. Although calretinin and calbindin-D28k were also found in numerous nonpyramidal neurons, both were also located in pyramidal-shaped neurons in layers V and VI (calretinin) and in layers II and III (calbindin) of the entorhinal cortex, suggesting that they play roles in projection neurons as well. Moreover, the high density of nonpyramidal neurons containing calcium-binding proteins in layers II and III of the entorhinal cortex suggests that they form an integral component of a network that controls the entorhinal outputs to the hippocampus. Furthermore, the largely nonoverlapping distributions of the parvalbumin-, calretinin-, and calbindin-ir neuronal populations in the entorhinal cortex indicate that each of them may modulate a different subset of topographically organized entorhinal outputs.

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Differential expression of calbindin and calretinin in the human fetal amygdala

Setzer

Ulfig

1999

Microsc. Res. Tech.

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Calbindin-D28k-immunoreactive cells and fibres in the human amygdaloid complex

Cited by 48 publications

References 67 publications

Subpopulations of neurons expressing parvalbumin in the human amygdala

Subpopulations of neurons expressing parvalbumin in the human amygdala

Distribution of parvalbumin-, calretinin-, and calbindin-D28k-immunoreactive neurons and fibers in the human entorhinal cortex

Differential expression of calbindin and calretinin in the human fetal amygdala

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