Calcification as an indicator of osteoinductive capacity of biomaterials in osteoblastic cell cultures

Declercq, Heidi; Verbeeck, Ronald; Ridder, Leo I.F.J.M. De; Schacht, Etienne; Cornelissen, Maria

doi:10.1016/j.biomaterials.2005.01.025

Cited by 128 publications

(99 citation statements)

References 37 publications

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“…This subsequently promoted bone regeneration as well as restored functions of the defected teeth. Declercq et al (2005) have shown that periosteum cells could differentiate into bone and play an important role in bone regeneration and the healing of bony defect or fracture. The same authors also indicated that periosteal cells express markers of mesenchymal stem cells and under specific conditions, they can differentiate to the chondrocyte, osteoblast, adipocyte, and skeletal myocyte lineages in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 99%

Application of autologous periosteal cells for the regeneration of class III furcation defects in Beagle dogs

Jiang

Lin

et al. 2010

Cytotechnology

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The aim of this study was to evaluate the healing of class III furcation defects following transplantation of autogenous periosteal cells combined with b-tricalcium phosphate (b-TCP). Periosteal cells obtained from Beagle dogs' periosteum explant cultures, were inoculated onto the surface of b-TCP. Class III furcation defects were created in the mandibular premolars. Three experimental groups were used to test the defects' healing: group A, b-TCP seeded with periosteal cells were transplanted into the defects; group B, b-TCP alone was used for defect filling; and group C, the defect was without filling materials. Twelve weeks post surgery, the tissue samples were collected for histology, immunohistology and X-ray examination. It was found that both the length of newly formed periodontal ligament and the area of newly formed alveolar bone in group A, were significantly increased compared with both group B and C. Furthermore, both the proportion of newly formed periodontal ligament and newly formed alveolar bone in group A were much higher than those of group B and C. The quantity of cementum and its percentage in the defects (group A) were also significantly higher than those of group C. These results indicate that autogenous periosteal cells combined with b-TCP application can improve periodontal tissue regeneration in class III furcation defects.

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Section: Discussionmentioning

confidence: 99%

Application of autologous periosteal cells for the regeneration of class III furcation defects in Beagle dogs

Jiang

Lin

et al. 2010

Cytotechnology

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“…Earlier reports indicate osteogenic differentiation of UCBMSCs by day 21 (De Schauwer et al 2012;Koch et al 2007;Schuh et al 2009). In addition, we also demonstrated the expression of Runx2 (transcription factor for early osteogenesis) and Osteocalcin (a non-collagenous protein specific for late osteoblast) on day 14 post-induction of differentiation (Declercq et al 2005).…”

Section: Discussionmentioning

confidence: 71%

Phenotypical and functional characteristics of mesenchymal stem cells derived from equine umbilical cord blood

et al. 2014

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Mesenchymal stem cells (MSCs) offer promise as therapeutic aid in the repair of tendon and ligament injuries in race horses. Fetal adnexa is considered as an ideal source of MSCs due to many advantages, including non-invasive nature of isolation procedures and availability of large tissue mass for harvesting the cells. However, MSCs isolated from equine fetal adnexa have not been fully characterized due to lack of species-specific markers. Therefore, this study was carried out to isolate MSCs from equine umbilical cord blood (UCB) and characterize them using cross-reactive markers. The plastic-adherent cells could be isolated from 13 out of 20 (65 %) UCB samples. The UCB derived cells proliferated till passage 20 with average cell doubling time of 46.40 ± 2.86 h. These cells expressed mesenchymal surface markers but did not express haematopoietic/ leucocytic markers by RT-PCR and immunocytochemistry. The phenotypic expression of CD29, CD44, CD73 and CD90 was shown by 96.36 ± 1.28, 93.40 ± 0.70, 73.23 ± 1.29 and 46.75 ± 3.95 % cells, respectively in flow cytometry, whereas, reactivity against the haematopoietic antigens CD34 and CD45 was observed only in 2.4 ± 0.20 and 0.1 ± 0.0 % of cells, respectively. Osteogenic and chondrogenic differentiation could be achieved using established methods, whereas the optimum adipogenic differentiation was achieved after supplementing media with 15 % rabbit serum and 20 ng/ml of recombinant human insulin. In this study, we optimized methodology for isolation, cultural characterization, differentiation and immunophenotyping of MSCs from equine UCB. Protocols and markers used in this study can be employed for unequivocal characterization of equine MSCs.

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“…The most stand-alone method of determining osteoblast differentiation besides analyzing mRNA is the observation of a cell-mediated calcified ECM. 71 We determined calcium content of each experimental group, and the osteomimetic scaffolds expressed the highest amount compared with the 2D control and Geltrex-coated PLGA scaffolds. Our study showed that 3D microenvironments produced from microsphere-sintered PLGA scaffolds generates a higher level of hESC differentiation into osteogenic lineage compared with cells grown on 2D tissue culture plates.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Differentiation of Human Embryonic Stem Cells on Extracellular Matrix-Containing Osteomimetic Scaffolds for Bone Tissue Engineering

Rutledge

Cheng

Pryzhkova

et al. 2014

Tissue Engineering Part C: Methods

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Current methods of treating critical size bone defects include autografts and allografts, however, both present major limitations including donor-site morbidity, risk of disease transmission, and immune rejection. Tissue engineering provides a promising alternative to circumvent these shortcomings through the use of autologous cells, three-dimensional scaffolds, and growth factors. We investigated the development of a scaffold with native bone extracellular matrix (ECM) components for directing the osteogenic differentiation of human embryonic stem cells (hESCs). Toward this goal, a microsphere-sintering technique was used to fabricate poly(lactic-co-glycolic acid) (PLGA) scaffolds with optimum mechanical and structural properties. Human osteoblasts (hOBs) were seeded on these scaffolds to deposit bone ECM for 14 days. This was followed by a decellularization step leaving the mineralized matrix intact. Characterization of the decellularized PLGA scaffolds confirmed the deposition of calcium, collagen II, and alkaline phosphatase by osteoblasts. hESCs were seeded on the osteomimetic substrates in the presence of osteogenic growth medium, and osteogenicity was determined according to calcium content, osteocalcin expression, and bone marker gene regulation. Cell proliferation studies showed a constant increase in number for hESCs seeded on both PLGA and ECM-coated PLGA scaffolds. Calcium deposition by hESCs was significantly higher on the osteomimetic scaffolds compared with the control groups. Consistently, immunofluorescence staining demonstrated an increased expression of osteocalcin in hESCs seeded on ECM-coated osteomimetic PLGA scaffolds. Gene expression analysis of RUNX2 and osteocalcin further confirmed osteogenic differentiation of hESCs at the highest expression level on osteomimetic PLGA. These results together demonstrate the potential of PLGA scaffolds with native bone ECM components to direct osteogenic differentiation of hESCs and induce bone formation.

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Calcification as an indicator of osteoinductive capacity of biomaterials in osteoblastic cell cultures

Cited by 128 publications

References 37 publications

Application of autologous periosteal cells for the regeneration of class III furcation defects in Beagle dogs

Application of autologous periosteal cells for the regeneration of class III furcation defects in Beagle dogs

Phenotypical and functional characteristics of mesenchymal stem cells derived from equine umbilical cord blood

Enhanced Differentiation of Human Embryonic Stem Cells on Extracellular Matrix-Containing Osteomimetic Scaffolds for Bone Tissue Engineering

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