Calcitonin and Glucose Uptake by lsolated Rat Diaphragm

Abstract

145Recently, an impairment of glucose assimilation and insulin output produced by calcitonin has been demonstrated in men (Zieg/er, Bel/winkel, Schmidtchen and Minne 1972). A possible competitive action of calcitonin to insulin was studied by means of isolated diaphragm museie method (Gemil/ 1941, Vallanc~Owen 1954. The diaphragms were excised from male rats weighing 100 gm and incubated in 2 ml KrebsHenseleit buffer under a gas phase (95% oxygen -5% carbon dioxide) for 90 minutes with an initial glucose content of 3 mg/mL Glucose uptake was determined with Somogyl's reagent, and the results are expressed in PI!, of glucose/l0 mg dried diaphragm.Synthetic salmon calcitonin (CTS) had no effect on the spontaneous glucose uptake <F~re). but inhibited significantly the Figure.References J crs (lU (ß)

Calcitonin stimulates gluconeogenesis in fasted rats.

“…CT inhibits glucose uptake stimulated by insulin in diaphragm muscle of rats, and that the effect of CT is not mediated by hypocalcemia (Gozarin andFlorescu, 1973 and1974). On the other hand, we have found that CT increases glucose concentration in the serum of fed and fasted rats (Yamaguchi and Yamamoto, 1977).…”