Calibration of MTT assay in proton beams using radiochromic films

Moftah, Belal; Aldelaijan, Saad; Shehadeh, Mohammad; Alzorkany, F.; Alrumayan, F.; Al-Shabanah, Mohammad; Seuntjens, Jan; Tomic, Nada; Dević, Slobodan

doi:10.1016/j.ejmp.2020.08.003

Cited by 1 publication

(1 citation statement)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The indirect cytotoxicity of the CBM/CMC, CBM/CMC/EEO, CBM/CMC/GEO and CBM/CMC/CEO hydrogels was evaluated using the MTT assay [ 23 , 24 ]. Briefly, the freeze-dried hydrogels were sterilized by exposure to the UV light for 2 h. Subsequently, hydrogel extracting liquid solutions were prepared by soaking the hydrogels at different concentrations (1, 10, 100, 1000, 10 000 μg/mL) in DMEM containing serum at 37°C for 24 h. After sterilization with a 0.22 μm filter, the culture medium in the 96-well plates was removed by using fresh DMEM culture medium as well as the extracting liquids corresponding to the CBM/CMC, CBM/CMC/EEO, CBM/CMC/GEO and CBM/CMC/CEO hydrogels for 24 h. Subsequently, 20 μL of 5 mg/mL MTT solution was added to the wells.…”

Section: Methodsmentioning

confidence: 99%

Antibacterial polysaccharide-based hydrogel dressing containing plant essential oil for burn wound healing

et al. 2021

View full text Add to dashboard Cite

Background Polysaccharide-based hydrogels have been developed for many years to treat burn wounds. Essential oils extracted from aromatic plants generally exhibit superior biological activity, especially antibacterial properties. Studies have shown that antibacterial hydrogels mixed with essential oils have great potential for burn wound healing. This study aimed to develop an antibacterial polysaccharide-based hydrogel with essential oil for burn skin repair. Methods Eucalyptus essential oil (EEO), ginger essential oil (GEO) and cumin essential oil (CEO) were employed for the preparation of effective antibacterial hydrogels physically crosslinked by carboxymethyl chitosan (CMC) and carbomer 940 (CBM). Composite hydrogels were prepared and characterized using antimicrobial activity studies, Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, gas chromatography-mass spectrometery, rheological analysis, viscosity, swelling, water loss rate and water vapor transmission rate studies. In addition, the biocompatibility of hydrogels was evaluated in vivo by cytotoxicity and cell migration assays and the burn healing ability of hydrogels was tested in vivo using burn-induced wounds in mice. Results The different essential oils exhibited different mixing abilities with the hydrogel matrix (CMC and CBM), which caused varying levels of reduction in essential oil hydrogel viscosity, swelling and water vapor transmission. Among the developed hydrogels, the CBM/CMC/EEO hydrogel exhibited optimal antibacterial activities of 46.26 ± 2.22% and 63.05 ± 0.99% against Staphylococcus aureus and Escherichia coli, respectively, along with cell viability (>92.37%) and migration activity. Furthermore, the CBM/CMC/EEO hydrogel accelerated wound healing in mouse burn models by promoting the recovery of dermis and epidermis as observed using a hematoxylin–eosin and Masson’s trichrome staining assay. The findings from an enzyme-linked immunosorbent assay demonstrated that the CBM/CMC/EEO hydrogel could repair wounds through interleukin-6 and tumor necrosis factor-α downregulation and transforming growth factor-β, vascular endothelial growth factor (VEGF) and epidermal growth factor upregulation. Conclusions This study successfully prepared a porous CBM/CMC/EEO hydrogel with high antibacterial activity, favorable swelling, optimal rheological properties, superior water retention and water vapor transmission performance and a significant effect on skin repair in vitro and in vivo. The results indicate that the CBM/CMC/EEO hydrogel has the potential for use as a promising burn dressing material for skin burn repair.

show abstract

Section: Methodsmentioning

confidence: 99%