Calibration: Sampling Density and Spatial Resolution

Young, Ian T.

doi:10.1002/0471142956.cy0206s05

Cited by 3 publications

(4 citation statements)

References 14 publications

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“…The sampling densities along both the horizontal and the vertical directions are preferably the same. 33 The sampling densities of the MEM-FLIM camera and the reference camera are measured by using a stage micrometer. The 20×, 0.5-NA objective lens is used in the experiment.…”

Section: Sampling Densitymentioning

confidence: 99%

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Modulated electron-multiplied fluorescence lifetime imaging microscope: all-solid-state camera for fluorescence lifetime imaging

et al. 2012

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Abstract.We have built an all-solid-state camera that is directly modulated at the pixel level for frequency-domain fluorescence lifetime imaging microscopy (FLIM) measurements. This novel camera eliminates the need for an image intensifier through the use of an application-specific charge coupled device design in a frequency-domain FLIM system. The first stage of evaluation for the camera has been carried out. Camera characteristics such as noise distribution, dark current influence, camera gain, sampling density, sensitivity, linearity of photometric response, and optical transfer function have been studied through experiments. We are able to do lifetime measurement using our modulated, electron-multiplied fluorescence lifetime imaging microscope (MEM-FLIM) camera for various objects, e.g., fluorescein solution, fixed green fluorescent protein (GFP) cells, and GFP-actin stained live cells. A detailed comparison of a conventional microchannel plate (MCP)-based FLIM system and the MEM-FLIM system is presented. The MEM-FLIM camera shows higher resolution and a better image quality. The MEM-FLIM camera provides a new opportunity for performing frequency-domain FLIM.

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Section: Sampling Densitymentioning

confidence: 99%

“…Using the method described in Ref. 33, the OTF can be calculated from the edge response. Our measurements are made in both the horizontal direction and the vertical direction.…”

Section: Resolutionmentioning

confidence: 99%

Modulated electron-multiplied fluorescence lifetime imaging microscope: all-solid-state camera for fluorescence lifetime imaging

et al. 2012

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“…For a periodic image, the visibility can be accurately calculated from the Fourier-transformed images (Figs. 3(c) and 3(d) by comparing the ratio of the intensity of the side-lobes to the intensity of zeroorder diffraction [21]. It should be mentioned that due to the limited numerical aperture of the collection optics, light is collected only from the zero-order transmission through the holearray.…”

Section: Resultsmentioning

confidence: 99%

Measuring the wavelength-dependent divergence of transmission through sub-wavelength hole-arrays by spectral imaging

Docter¹,

Young²,

Piciu³

et al. 2006

Opt. Express

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We present a study on the far-field patterns of light transmitted through sub-wavelength metallic hole-arrays. Spectral imaging measurements are used here on hole arrays for the first time. It provides both spatial and spectral information of the transmission in far-field. The visibility of the images, measured in two illumination modes: Köhler and collimated, is calculated for different planes in and out of focus. The transmission under collimated illumination reveals that 75% of the beam if non-divergent. The results are in agreement with the low divergence measured by Lezec [Science 297, 820 (2002)].

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“…In other words, spatial frequencies greater than f c cannot be transferred by a given optical system, which therefore is said to be band-limited. A more detailed discussion of OTF and PSF can be found in UNIT 2.6 (Young, 2001).…”

Section: Resolution In the Frequency Domainmentioning

confidence: 99%

Basics of Digital Microscopy

et al. 2018

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Modern digital microscopy combines the equipment of classical light microscopy with a computerized imaging system. The technique comprises image formation by optics, image registration by a camera, and saving of image data in a computer file. This chapter describes limitations that are particular to each of these processes, including optical resolution, efficiency of image registration, characteristics of image file formats, and data management. Further suggestions are given which serve, in turn, to help construct a set of guidelines aimed at optimization of digital microscopic imaging. © 2018 by John Wiley & Sons, Inc.

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Calibration: Sampling Density and Spatial Resolution

Cited by 3 publications

References 14 publications

Modulated electron-multiplied fluorescence lifetime imaging microscope: all-solid-state camera for fluorescence lifetime imaging

Modulated electron-multiplied fluorescence lifetime imaging microscope: all-solid-state camera for fluorescence lifetime imaging

Measuring the wavelength-dependent divergence of transmission through sub-wavelength hole-arrays by spectral imaging

Basics of Digital Microscopy

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