Calix[4]arenesulfonylamidines. Synthesis, structure and influence on Mg2+, ATP-dependent calcium pumps

Calixarene Derivatives for (Nano)Biotechnologies

“…[5,6] Further investigation of macrocycles 2, 3 showed that di-substituted calixarenes as well as model compounds 4 and 5 possess less activity (Table 1). So, inhibition efficiency of the Ca 2+ , Mg 2+ -ATPase of PM by the sulfonylamidine calix [4] arenes correlates with quantity of phenylsulfonylamidine groups on the calixarene upper rim. It should be noted that the inhibitory effects of the studied calix [4]arenes also depended on the relative location of phenylsulfonylamidine groups on the upper rim of the calix [4]arene "bowl".…”

“…So, inhibition efficiency of the Ca 2+ , Mg 2+ -ATPase of PM by the sulfonylamidine calix [4] arenes correlates with quantity of phenylsulfonylamidine groups on the calixarene upper rim. It should be noted that the inhibitory effects of the studied calix [4]arenes also depended on the relative location of phenylsulfonylamidine groups on the upper rim of the calix [4]arene "bowl". [7] Calixarene-bisamide 6 in concentration 100 µM acts multidirectionally on the functionally different Mg 2+ -dependent ATP-hydrolase enzymatic systems.…”

“…[4][5][6] It was shown that calixarene-tetrasulfonylamidine 1 reduces the transport activity of the ruthenium red insensitive, oxalate-stimulated, Mg 2+ , ATP-dependent calcium pump of sarcoplasmic reticulum by 75 %. A similar result was found for the Mg 2+ , ATP-dependent calcium pump of the plasma membrane (PM, I 0.5 =34.6 µМ).…”

Calix[4]arene C-90 and its analogs activate ATPase of the myometrium myosin subfragment-1

“…1) were synthesized and characterized using NMR techniques and IR spectroscopy in the Phosphoranes Chemistry Department of the Insti tute of Organic Chemistry, NAS of Ukraine headed by Corresponding member NAS of Ukraine Prof. V. I. Kalchenko. Synthesis of calix [4]arene C90 has been described previously [13,8]. Calix [4]arene studied in the work: C-90 (5,11,17,23- Calix [4]arenes were dissolved in dimethylsul foxide (DMSO) and diluted with water to the de sired concentration.…”

“…It uses the free energy of ATP hydrolysis deposited in ATP macroergic bonds for cyclic changes in the structure of the myosin head. The conformational changes in myosin active site due to ATP hydrolysis are enhanced with the assis tance of switch 1, switch 2, relay and converter and transferred to the regulatory domain -lever arm, which plays an essential role in the generation of force and movement [13]. As a result, myosin moves along the actin filament causing the muscle contrac tion.…”