Calves Born after Direct Transfer of Vitrified Bovine <i>In Vitro</i>‐produced Blastocysts Derived from Vitrified Immature Oocytes

Vieira, Arnaldo Diniz; Forell, Fabiana; Feltrin, Cristiano; Rodrigues, José Ariévilo Gurgel

doi:10.1111/j.1439-0531.2007.00899.x

Cited by 33 publications

(21 citation statements)

References 23 publications

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“…Moreover, vitrified in vitromatured oocytes formed second polar bodies and pronuclei, events that depend on release of intracellular Ca 2þ [38]. Deficiencies in the ability of the ER to reorganize following IVM and/or other components of cytoplasmic maturation could help explain the low developmental rates reported by others following oocyte cryopreservation and IVM [23,25,[28][29][30][31]. Additional causes of low developmental competence in some cryopreserved oocytes could be that the zona pellucida is modified, such that it hardens and prevents fertilization in some cases [3,7,11,15,47].…”

Section: Mouse Oocyte Vitrification 151mentioning

confidence: 94%

“…However, the reorganization of the ER that normally occurs during oocyte maturation was impaired by this process. Although vitrification of immature oocytes followed by in vitro maturation yields morphologically normal oocytes that exhibit some of the properties of early development following fertilization, cytoplasmic maturation is disrupted, and this could account for the reduced developmental potential observed previously following vitrification and in vitro maturation [23,25,[28][29][30][31].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the cryopreservation of GV-stage oocytes is an attractive alternative for freezing female gametes. Although studies [23][24][25][26][27][28][29] have shown that bovine oocytes frozen at the GV stage have lower survival rates and developmental competence than oocytes frozen at the MII stage, live births have been obtained using this procedure [23,24,[28][29][30]. Because the developmental competence of both cryopreserved GV-stage and MII-stage bovine oocytes is significantly lower than that of unfrozen controls, a more accurate comparison between freezing GV-stage vs. MII-stage bovine oocytes might be made after the overall cryopreservation procedure is improved.…”

Section: Introductionmentioning

confidence: 99%

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Maturation, Fertilization, and the Structure and Function of the Endoplasmic Reticulum in Cryopreserved Mouse Oocytes1

Lowther

Weitzman

Maier

et al. 2009

Biology of Reproduction

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Oocyte cryopreservation is a promising technology that could benefit women undergoing assisted reproduction. Most studies examining the effects of cryopreservation on fertilization and developmental competence have been done using metaphase IIstage oocytes, while fewer studies have focused on freezing oocytes at the germinal vesicle (GV) stage, followed by in vitro maturation. Herein, we examined the effects of vitrifying GVstage mouse oocytes on cytoplasmic structure and on the ability to undergo cytoplasmic changes necessary for proper fertilization and early embryonic development. We examined the endoplasmic reticulum (ER) as one indicator of cytoplasmic structure, as well as the ability of oocytes to develop Ca 2+ release mechanisms following vitrification and in vitro maturation. Vitrified GV-stage oocytes matured in culture to metaphase II at a rate comparable to that of controls. These oocytes had the capacity to release Ca 2+ following injection of inositol 1,4,5-trisphosphate, demonstrating that Ca 2+ release mechanisms developed during meiotic maturation. The ER remained intact during the vitrification procedure as assessed using the lipophilic fluorescent dye DiI. However, the reorganization of the ER that occurs during in vivo maturation was impaired in oocytes that were vitrified before oocyte maturation. These results show that vitrification of GV-stage oocytes does not affect nuclear maturation or the continuity of the ER, but normal cytoplasmic maturation as assessed by the reorganization of the ER is disrupted. Deficiencies in factors that are responsible for proper ER reorganization during oocyte maturation could contribute to the low developmental potential previously reported in vitrified in vitro-matured oocytes.assisted reproductive technology, gamete biology, in vitro fertilization, meiosis

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Section: Mouse Oocyte Vitrification 151mentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Maturation, Fertilization, and the Structure and Function of the Endoplasmic Reticulum in Cryopreserved Mouse Oocytes1

Lowther

Weitzman

Maier

et al. 2009

Biology of Reproduction

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show abstract

“…These negative effects have limited the widespread use of slow freezing techniques. Since the invention of vitrification by Rall and Fahy [10], it has been widely applied for the cryopreservation of human oocytes [11], a variety of domestic and laboratory animals [12][13][14][15][16][17][18][19][20][21], as well as other mammalian embryos [21][22][23][24][25][26][27][28][29][30]. Furthermore, vitrification is considered to be a better alternative to slow freezing cryopreservation [31].…”

mentioning

confidence: 99%

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

Moussa

Shen

Zhang

et al. 2014

Sci. China Life Sci.

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Cryopreservation techniques for mammalian oocytes and embryos have rapidly progressed during the past two decades, emphasizing their importance in various assisted reproductive technologies. Pregnancies and live births resulting from cryopreserved oocytes and embryos of several species including humans have provided proof of principle and led to the adoption of cryopreservation as an integral part of clinical in vitro fertilization. Considerable progress has been achieved in the development and application of the cryopreservation of mammalian oocytes and embryos, including preservation of the reproductive potential of patients who may become infertile, establishment of cryopreserved oocyte banks, and transport of oocytes and embryos internationally. However, the success rates are still far lower than those obtained with fresh oocytes and embryos, and there are still obstacles that need to be overcome. In this review, we address the major obstacles in the development of effective cryopreservation techniques. Such knowledge may help to eliminate these hurdles by revealing which aspects need improvement. Furthermore, this information may encourage further research by cryobiologists and increase the practical use of cryopreservation as a major part of assisted reproductive technologies for both humans and animal species. cryopreservation, mammals, oocytes, embryos, cryoinjuries Citation:Moussa M, Shu J, Zhang XH, Zeng FY. Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives. Sci China Life Sci, 2014, 57: 903 -914,

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“…However, oocytes are much more difficult to cryopreserve than embryos due to their morphology, higher cryosensitivity and low hydraulic conductivity (Leibo, 1980;Ambrosini et al, 2006). Although, several experiments have been performed using different vitrification protocols with matured and immature bovine oocytes (Vajta et al, 1998;Men et al, 2002;Albarracin 2005;Magnusson et al, 2008;Morato et al, 2008;Vieira et al, 2008;Sripunya et al, 2010, Zhou et al, 2010, survival rates after warming have been low, with embryo production rates between 0 to 13% (Vajta et al, 1998;Dinnyes et al, 2000;Morato et al, 2008;Sripunya et al, 2010;Zhou et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Vitrification of immature and matured bovine oocytes: effect of brilliant cresyl blue selection and hyaluronan addition

Rodríguez-Villamil¹,

Ongaratto²,

Moreira³

et al. 2016

Anim Reprod

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The aim of the present study was to evaluate the effect of brilliant cresyl blue (BCB) selection, the type of oocyte (immature or matured) and the use of hyaluronan in the vitrification solution on further embryo developmental competence. Oocytes (n = 1308) obtained from abattoir ovaries were classified by BCB stain. Control oocytes were maintained in holding media for 90 min and then subdivided to be placed into maturation media without any treatment or were vitrified. Immature or matured oocytes were vitrified by the solid-surface technique using two different vitrification solutions. VS1: composed of 10% ethylene glycol (EG) for 10 min followed by 20% EG + 0.2M trehalose for 30 sec and finally into 30% EG + 0.5M trehalose for 30 sec, or VS2 composed by 10% EG for 10 min, followed by 20% EG + 0.2M trehalose for 30 sec, and finally into 30% EG+ 0.5M trehalose + 0.1 g/ml hyaluronan for 30 sec. Oocytes were then loaded into Fyberplugs™ and vitrified. After one week, Fyberplugs™ were open and placed directly into (37°C) 0.5M sucrose solution for 5 min, then into 0.25M of sucrose for another 5 min and finally placed into maturation medium for in vitro production. Cleavage and development rates were examined on days 2 and 7 after fertilization, respectively. The blastocyst rate of vitrified oocytes selected as BCB + (5.5 ± 0.6%) were higher than those selected as BCB -(1.0 ± 0.4%) and those that were not selected by BCB (2.0 ± 1.1%; P < 0.001). Furthermore, immature vitrified oocytes had greater (P < 0.05) cleavage and blastocyst rates (44.8 ± 1.9% and 4.0 ± 0.6%) than matured vitrified oocytes (38.3 ± 2.8% and 2.5 ± 0.6%). Finally, the addition of hyaluronan to the vitrification solution had no significant effect on development rates. In conclusion, the selection of oocytes by BCB and the use of immature oocytes increase the development rates of vitrified-warmed oocytes.

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Calves Born after Direct Transfer of Vitrified Bovine In Vitro‐produced Blastocysts Derived from Vitrified Immature Oocytes

Cited by 33 publications

References 23 publications

Maturation, Fertilization, and the Structure and Function of the Endoplasmic Reticulum in Cryopreserved Mouse Oocytes1

Maturation, Fertilization, and the Structure and Function of the Endoplasmic Reticulum in Cryopreserved Mouse Oocytes1

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

Vitrification of immature and matured bovine oocytes: effect of brilliant cresyl blue selection and hyaluronan addition

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