Campylobacter jejuni e Campylobacter coli EM CARCAÇAS DE FRANGO RESFRIADAS E CONGELADAS

Cisco, Isabel Cristina; Tedesco, Denise Cristina; Perdoncini, Gustavo; Santos, Suelen Priscila; Rodrigues, Laura Beatriz; Santos, Luciana Ruschel dos

doi:10.1590/1089-6891v18e-42481

Cited by 4 publications

(2 citation statements)

References 11 publications

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“…De acordo com a Tabela 1, foi possível determinar a potencialidade de infecções promovidas pela água contaminada (Silva et al, 2012), pelo consumo de carnes cruas e mal cozidas (Feistel et al, 2013), sejam elas de origem suína (Andrade et al, 2014), carcaças e cortes de frango congelados e/ou resfriados (Cisco et al, 2017), carne bovina (Leão, 2018), por meio do leite cru e em alguns casos, recontaminações em leites pasteurizados (Monteiro et al, 2015), e até mesmo pelo contato direto com animais que colonizam a bactéria de maneira comensal (De Luca et al, 2019).…”

Section: Fonte: Elaborada Pelos Autores (2020)unclassified

Fatores associados ao impacto de gastroenterites por Campylobacter jejuni

Lima¹,

Oliveira

2020

RSD

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Campylobacter jejuni is a bacteria that is involved in the causality of several pathologies, from asymptomatic cases and others with great amplitude to the propensity of death. Its form of acquisition is facilitated, and the human being can become infected by the consumption of contaminated water and food, prepared incorrectly or under poor hygiene techniques. Because it has the possibility of adhesion and synthesis of toxins, there is a large-scale increase in its potential for pathogenicity. In Brazil, this microorganism is detected in cases of gastroenteritis in children. In developed countries it is the main cause of traveler's diarrhea. This work addresses the morphological characteristics of Campylobacter jejuni, its virulence and epidemiology factor, in addition to the possible diseases caused. To carry out the research, a literature review was carried out, based on publications indexed in the databases: Scielo Google Scholar and Pubmed, published between 2010 and 2020. The descriptors used were: Campylobacter Infections; Gastroenteritis; Campylobacter jejuni, where there were 731 articles, with only 44 selected. The Polymerase Chain Reaction, for measuring the genes of the CDT, is a differential for infectious, always combined with other laboratory and clinical data for a conclusive diagnosis. The use of antimicrobials for the treatment, prevention and / or growth of animals for slaughter has led to an increase in cases of resistance related to microbiota, including C. jejuni.

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Section: Fonte: Elaborada Pelos Autores (2020)unclassified

Fatores associados ao impacto de gastroenterites por Campylobacter jejuni

Lima¹,

Oliveira

2020

RSD

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“…Among the routes of human infection, chicken meat represents the main source ( EFSA, 2016 ; CDC, 2017 ), and in Brazil, poultry farming stands out for the title of largest exporter and third largest producer ( ABPA, 2022 ). But, due to the difficulties in diagnosing campylobacteriosis and underreporting problems in the country, the few records of the disease in humans are related to occasional research ( Cisco et al., 2017 ).…”

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confidence: 99%

Roles of viable but non-culturable state in the survival of Campylobacter jejuni

Santos

Rossi

Braz

et al. 2023

Front. Cell. Infect. Microbiol.

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Despite being considered fragile and fastidious, Campylobacter jejuni is the most prevalent cause of foodborne bacterial gastroenteritis, and chicken meat is considered the main vehicle of transmission to humans. This agent can survive adverse conditions in the form of biofilms, but extreme stress (nutritional, oxidative and thermal) promotes the acquisition of a state called viable but not culturable (VBNC). The emergence of this pathogen worldwide and the recent international requirements in its control instigated us to qualitatively and quantitatively estimate the time required for the acquisition of the VBNC form in 27 strains of C. jejuni, characterize morphological aspects, determine its adaptive and invasive potential and perform comparative metabolomic evaluation. Extreme stress promoted the complete acquisition of the VBNC form in a mean time of 26 days. Starting from an average initial count of 7.8 log CFU/mL, the first four days determined the greatest average reduction of the culturable form of 3.2 log CFU/mL. The scanning and transmission image analyses showed a transition from the typical viable form (VT) to the VBNC form, with initial acquisition of the straight rod shape, followed by loss of the flagella and subdivision into two to 11 imperfect cocci arranged in a chain and rich in cellular content, until their individual release. RT-PCR identified the presence of ciaB and p19 transcripts in the 27 cultivable C. jejuni strains, a character maintained in the VBNC form only for p19 and in 59.3% (16/27) of the VBNC strains for the ciaB gene. The average inoculation of 1.8 log CFU/mL of C. jejuni VBNC into primary chicken embryo hepatocyte cells promoted the occurrence of apoptosis processes significantly after 24 hours of contact by one of the strains tested. In C. jejuni VBNC, we detected higher expression of metabolites linked to protective and adaptation mechanisms and of volatile organic precursor compounds indicative of metabolism interruption. The oscillations in the time of acquisition of the VBNC form together with the presence of transcripts for ciaB and p19, the identification of cell lysis and metabolites that ensure the maintenance of the pathogen alert to the fact that C. jejuni VBNC remains virulent and adapted to stress, which makes evident the potential danger of this latent form, which is not detectable by official methodologies.

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