Candida: Biofilm formation and antifungal resistance

Sharma, Shraddha; Singh, Shiv Mohan; Mane, Gajanan; Pote, Satish T.; Patole, Milind S.; Sharma, Ram Krishan

doi:10.1016/b978-0-323-99977-9.00024-7

Cited by 9 publications

(15 citation statements)

References 104 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…20 The MICs of FLC, MCF, and CAS against E. dermatitidis exceeded 16 mg/L as previously described. 21,22 PSC, VRC, AmB, TRB, and ITC showed potent inhibitory ability from 0.031 to 0.25 mg/L at MIC50 and MIC90 as assessed using OD530 and WST-1.…”

Section: Antifungal Activity Of Clinically Used Drugs Against E Derma...mentioning

confidence: 99%

“…Bacterial and fungal biofilms form in infected organs, particularly on medical devices such as intravascular catheters, artificial heart valves, and artificial joints, causing intractable chronic infections that are resistant to antibiotics and antifungals. 22 It has been reported that E. dermatitidis can produce biofilm formation. 23 E. dermatitidis was incubated with or without A549 cells in a glass-bottomed slide chamber plate.…”

Section: Inhibition and Eradication Of E Dermatitidis-induced Biofilmmentioning

confidence: 99%

See 1 more Smart Citation

Antifungal potency of terbinafine as a therapeutic agent against Exophiala dermatitidis in vitro

Nakamura,

Yoshinouchi,

Okumura

et al. 2024

Preprint

View full text Add to dashboard Cite

Background: Exophiala dermatitidis (E. dermatitidis), which causes skin infections or respiratory diseases, is occasionally fatal in immunocompromised patients. Objectives: Here, we report the unique antifungal potency of terbinafine (TRB), which targets squalene epoxidase, against E. dermatitidis (SQLEED) using various in vitro approaches. Methods: Based on the human SQLE crystal structure, we created a structure model of SQLEED using SWISS-MODEL and determined the best-fitting model of TRB to the SQLEED. The versatile antifungal activities, including fungicidal activity, biofilm inhibition, biofilm eradication activity, and the combination effect of TRB, posaconazole (PSC), and amphotericin B (AmB) with great antifungal potency against E. dermatitidis were evaluated using crystal violet and cell viability assay. Results: Clinically isolated E. dermatitidis increased most vigorously at 30°C but decreased at 40°C. E. dermatitidis hyphae elongated and attached to a cell scaffold, forming a membrane-like biofilm that was distinct from the cell-free biofilm. In the binding model, TRB formed an H-bond with Y102 and was surrounded by key amino acid residues of SQLEED corresponding to TRB-resistant mutations in Trichophyton rubrum, showing an appropriate interaction. Among TRB, PSC, and AmB with potent antifungal activities, TRB and PSC showed more potent antibiofilm activities than AmB. In addition, TRB and PSC exhibited residual potency without incubation against E. dermatitidis, decreasing the growth at lower concentrations than AmB. In contrast, AmB exhibited strong time-dependent killing and eradication activities. The combination of TRB and PSC was more effective than that of TRB and AmB or PSC and AmB in vitro. Conclusions: Although the tissue migration of TRB must be considered, these data suggest that TRB and PSC may be useful agents and a potent combination in severely immunocompromised patients with refractory and systemic E. dermatitidis infection.

show abstract

Section: Antifungal Activity Of Clinically Used Drugs Against E Derma...mentioning

confidence: 99%

Section: Inhibition and Eradication Of E Dermatitidis-induced Biofilmmentioning

confidence: 99%

Antifungal potency of terbinafine as a therapeutic agent against Exophiala dermatitidis in vitro

Nakamura,

Yoshinouchi,

Okumura

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…A wide range of substituted cationic porphyrinshave been used to mediate PDI of diverse species of pathogens [13][14][15]. Ithas also been reported that cationic PSs are particularly efficient at eradicating biofilms developed by Gram-positive bacteria [9].…”

Section: Introductionmentioning

confidence: 99%

Laser NIR irradiation enhances antimicrobial photodynamic inactivation of biofilms ofStaphylococcus aureus

Mamone,

Tomás,

Di Venosa

et al. 2024

Preprint

View full text Add to dashboard Cite

Photodynamic inactivation (PDI) utilizes a photosensitizer (PS) activated by visible light to generate reactive oxygen species (ROS), to kill bacteria. PDI is effective against planktonic microorganisms, but biofilms are less sensitive due to limited PS and oxygen penetration. Near-infrared treatment (NIRT), involve the use of near-infrared light to kill bacteria either via thermal effects or ROS production. Our objective was to enhance S. aureus biofilm's sensitivity to PDI by pre-treating with NIR irradiation before visible light exposure. In an in vitro biofilm model, laser NIRT (980 nm) followed by exposure to PDI, showed a synergistic effect on bacterial viability loss (4-log CFU vs 1-log loss with individual treatments). Interestingly, pre-heating liquid medium had no significant impact on PDI efficacy, suggesting that both thermal and non-thermal effects of NIR may be involved. NIRT increased PS uptake, induced clefts in the biofilm matrix, and released bacterial cells from the biofilm. NIRT induced a transient increase in the temperature to 46°C of in vitro cultures, however under the same conditions, when mice were irradiated, skin temperature rose to 37°. Our findings suggest that NIR irradiation serves as a complementary treatment to PDI, allowing reducing PS concentration, and highlighting its potential as an effective and resource-efficient antibacterial approach.

show abstract

“…The biofilm microorganisms are resistant to environmental stress factors, tolerant to antimicrobial therapy, ensured by a complex array of resistance mechanisms. The realization of resistance occurs through mechanisms inherent to both planktonic cells (antibiotic inactivation by enzymes, hyperactivity of efflux pumps, modification of target structure, reduction of the cell membrane permeability) and specific mechanisms, such as the pre sen ce of the matrix that prevents the AMAs penetration into the biofilm at a concentration sufficient to inhibit cells, the activation of Quorum Sensing (QS) systems, and the formation of persister cells with a slowed metabolism [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…Currently, in addition to research on the antibiofilm action of modern AMAs, there is an initiative to explore the potential of new substances to block the synthesis of virulence factors and specific matrix enzymes of biofilms [6,8]. It has been established that DNAse, proteinase K, and trypsin affect the matrix eDNA, disrupting the matrix structure and density [9].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and antibiofilm activity of novel 1,4-dihydropyrido[1,2-a]pyrrolo[2,3-d]pyrimidine-2-carboxamides

Muzychka,

Humeniuk,

Boiko

et al. 2024

Biopolym. Cell

View full text Add to dashboard Cite

Synthesis of novel alkyl-substituted 4-oxo-1,4-dihydropyrido [1,2-a]pyrrolo [2,3-d]pyrimidine-2-carboxamides and evaluation of their antibiofilm activity in vitro. Methods. Organic synthesis, analytical and spectral methods, broth microilution method, biofilm formation on abiotic surface. Results. A simple and efficient method for the synthesis of new 1,4-dihydropyrido[1,2-a]pyrrolo[2,3-d]pyrimidine-2-carboxylic acid derivatives was developed. The results of antibiofilm activity screening showed that among the synthesized alkyl-substituted 1,4-dihydropyrido[1,2-a]pyrrolo[2,3-d]pyrimidine-2-carboxamides there are the compounds capable of disrupting the formation of biofilm of methicillin-resistant strain S. aureus 222, E. coli 311 and P. aeruginosa 449. Compound 6g is active against biofilms of E. coli 311, biomass decreases by 91.2 %, and against S. aureus 222 (reduction by 54.0 %). Compound 6d is active against biofilms of P. aeruginosa 449 and S. aureus 222 (reduction by 78.7 % and 50.2 %, respectively). Conclusions. A series of novel substituted 1-alkyl-4-oxo-1,4dihydropyrido[1,2-a]pyrrolo[2,3-d]pyrimidine-2-carboxamides were synthesized. The acti vity of the synthesized pyrido[1,2-a]pyrrolo [2,3-d]pyrimidines towards the S. aureus 222, E. coli 311 and P. aeruginosa 449 biofilm formation was investigated, and the compounds with pronounced antibiofilm activity were found. K e y w o r d s: pyrido[1,2-a]pyrrolo [2,3-d]pyrimidines, pyrido[1,2-a]pyrimidine-3-carbaldehydes, synthesis, antibiofilm activity.

show abstract

Candida: Biofilm formation and antifungal resistance

Cited by 9 publications

References 104 publications

Antifungal potency of terbinafine as a therapeutic agent against Exophiala dermatitidis in vitro

Antifungal potency of terbinafine as a therapeutic agent against Exophiala dermatitidis in vitro

Laser NIR irradiation enhances antimicrobial photodynamic inactivation of biofilms ofStaphylococcus aureus

Synthesis and antibiofilm activity of novel 1,4-dihydropyrido[1,2-a]pyrrolo[2,3-d]pyrimidine-2-carboxamides

Contact Info

Product

Resources

About