Capillary blood count analyses in clinical practice: a safe, reliable and valid method<sup>1</sup>

Schalk, Enrico; Scheinpflug, Katrin; Mohren, Martin

doi:10.1515/jlm.2009.051et

Cited by 4 publications

(10 citation statements)

References 17 publications

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“…In contrast to our study, they found no clinically significant differences between these tubes for CBC use [16]. In the literature, there are several studies that compare capillary blood taken from a SVT and venous blood taken from a vacuum tube; however, the results are conflicting [17][18][19]. In our study, venous blood collection was used for both tubes.…”

Section: Discussioncontrasting

confidence: 95%

Comparison of small-volume tubes with vacuum blood tubes for complete blood count

Ünal¹

2019

J Med Biochem

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Comparison of small-volume tubes and vacuum blood tubes for complete blood count T he complete blood count (CBC) is one of the most commonly requested clinical laboratory tests [1]. Accurate measurement of the CBC is essential for the correct diagnosis, treatment, and follow-up of patients with hematological disorders. These measurements are now almost fully automated, and the right interpretation of the clinical significance of the results requires knowledge of the pre-analytical phases [2]. The blood collection tubes used for a CBC should contain anticoagulant ethylenediaminetetraacetic acid (EDTA), according to the recommendation of the Clinical & Laboratory Standards Institute (CLSI) [3]. EDTA salts are used for the anticoagulation of whole blood for hematological analyses because the cellular components of the blood are well preserved by EDTA [4]. Today, a CBC is typically performed with whole blood collected in tubes containing dipotassium EDTA (K2EDTA) as an anticoagulant. Blood collection tube additives and components are an important component of the pre-analytical phase in terms of a potential influence on laboratory test results. Although Objectives: A complete blood count (CBC) is one of the most commonly requested clinical laboratory tests. Vacuum blood tubes are used routinely, and now, new small-volume tubes (SVTs) containing dipotassium ethylenediaminetetraacetic acid (K2EDTA) are also in use. The aim of this research was to compare SVTs with vacuum blood tubes for use in a CBC. Methods: Venous blood samples were taken from 40 healthy volunteers and were collected in BD Vacutainer (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) K2EDTA tubes and BD Microtainer (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) K2EDTA tubes. CBC parameters were analyzed using an ABX Pentra DF 120 device (Horiba, Ltd., Kyoto, Japan). Results: Red blood cells (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), and basophil (BASO) levels were found to be statistically significantly higher, while platelet (PLT) levels were determined to be statistically significantly lower in the SVT analyses compared with those of the vacuum blood tubes. When the percentage difference was compared with the total allowable error, RBC, HGB, HCT, MCH, MCHC, red cell distribution width, white blood cell count, neutrophil, lymphocyte, monocyte, eosinophil, and BASO values demonstrated a general trend of positive bias, while PLT values demonstrated a general trend of negative bias on a Bland-Altman bias plot. Conclusion: The results of this study demonstrated that venous blood samples from SVTs generated a significant bias in the CBC results. In addition, clotted samples were observed more frequently among those collected in SVTs in this study. The design of SVTs may have an important and detrimental effect on the accuracy and efficacy of the results of a CBC.

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Section: Discussioncontrasting

confidence: 95%

Comparison of small-volume tubes with vacuum blood tubes for complete blood count

Ünal¹

2019

J Med Biochem

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“…With the use of new-generation analysers, no significant differences were found when comparing venous vs. capillary blood in a study performed using a similar analyser to ours (Sysmex XT-2000i) [7]. Previous studies have demonstrated that there is a statistically significant difference in venous vs. capillary peripheral blood samples; however, these differences were greater in children than in adults [8] and do not have clinical importance nor do they play a defining role in changing diagnostic or therapeutic decisions. Studies performed before now included cohorts of patients suffering from several advanced haematological diseases, similar to the cohort presented herein, albeit important differences were the use of a nonautomated lancet and the fact that three different operators were imitating a real-life setting.…”

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confidence: 46%

No clinically relevant differences between capillary and venous blood cell counts in adult haematological patients using a nonautomated lancet

Herrera-Rojas

Tarín‐Arzaga

León

et al. 2015

Int J Lab Hematology

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“…thromboplastin) due to samplinginduced tissue injury with consecutive changes in cell activity and concentration. 21 This demonstrates the importance of differentiating between capillary and venous peripheral blood to avoid misinterpretation of test results. 17,21 While optimum guidance of clinical decisions would theoretically require separate decision limits for venous and capillary samples, this is not feasible in clinical practice.…”

Section: Introductionmentioning

confidence: 99%

Differences between capillary and venous blood counts in children—A data mining approach

Becker

Gscheidmeier

Groß

et al. 2022

Int J Lab Hematology

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Background Capillary sampling of blood counts is a well‐established alternative to venipuncture in paediatrics. However, the sampling method has to be considered when interpreting test results, as measurements differ. Ethical and practical considerations prevent simultaneous venous and capillary sample acquisition in comprehensive paediatric cohorts that span all ages for the purpose of a direct method comparison, resulting in uncertainty regarding the interpretation of capillary test results. Methods We applied a data mining method to calculate the differences between capillary and venous blood count analytes using laboratory data collected during patient care. We examined 486 401 blood counts performed between 2010 and 2017 in two German paediatric tertiary care centers in children from birth to 18 years analysed on SYSMEX XE‐2100 and SYSMEX XE‐5000 devices, and analysed the differences between capillary and venous test results in 15 218 paired samples performed within 24 h. Results We identified the mean systematic differences between capillary and venous (capillary–venous) test results for haemoglobin (+6.5 g/L), haematocrit (+2.38%), platelet count (−7.01 × 109/l), red cell count (+0.18 × 1012/L), white cell count (−0.64 × 109/L), mean corpuscular cell volume (+2.07 fl), mean corpuscular haemoglobin (+0.33 pg), mean corpuscular haemoglobin concentration (−4.4 g/L) and red cell distribution width (+0.40%). The effect of age on these mean deltas is negligible, while the levels of test results influence the difference between capillary and venous test results in most analytes. Conclusions Our results improve guidance regarding the interpretation of capillary test results for children of all ages and in both physiological and pathological ranges.

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Capillary blood count analyses in clinical practice: a safe, reliable and valid method¹

Cited by 4 publications

References 17 publications

Comparison of small-volume tubes with vacuum blood tubes for complete blood count

Comparison of small-volume tubes with vacuum blood tubes for complete blood count

No clinically relevant differences between capillary and venous blood cell counts in adult haematological patients using a nonautomated lancet

Differences between capillary and venous blood counts in children—A data mining approach

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Capillary blood count analyses in clinical practice: a safe, reliable and valid method1

Cited by 4 publications

References 17 publications

Comparison of small-volume tubes with vacuum blood tubes for complete blood count

Comparison of small-volume tubes with vacuum blood tubes for complete blood count

No clinically relevant differences between capillary and venous blood cell counts in adult haematological patients using a nonautomated lancet

Differences between capillary and venous blood counts in children—A data mining approach

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Capillary blood count analyses in clinical practice: a safe, reliable and valid method¹