Capillary electrophoresis in clinical and forensic analysis

Heeren, Franz von; Thormann, Wolfgang

doi:10.1002/elps.1150181232

Cited by 55 publications

(14 citation statements)

References 111 publications

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“…The forensic DNA analysis is made through multiplex PCR amplification of 10-16 STRs or more, followed by automated sequencing equipment, such as capillary electrophoresis (CE) [16].…”

Section: Autosomal Str Profilingmentioning

confidence: 99%

Molecular Genetics and its Applications in Forensic Sciences

Dumache¹,

Ciocan²,

Muresan³

et al. 2016

Forensic Analysis - From Death to Justice

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The way to medico legal identification was open at the end of the twenty-first century by the "digital fingerprinting" represented by the multifactorial phenotypical trait, determined by both polygenic and environmental factors, followed by group-specific antigens, or with specificity for blood and tissue, and ending with the DNA molecule in use today. Because of this aspect, the framework of modern forensic medicine includes a new field, that of forensic genetics, that mostly involves working with investigations that have human genotype identification as a goal.

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“…The forensic DNA analysis is made through multiplex PCR amplification of 10-16 STRs or more, followed by automated sequencing equipment, such as capillary electrophoresis (CE) [16].…”

Section: Autosomal Str Profilingmentioning

confidence: 99%

Molecular Genetics and its Applications in Forensic Sciences

Dumache¹,

Ciocan²,

Muresan³

et al. 2016

Forensic Analysis - From Death to Justice

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“…[4][5][6][7][8][9][10][11] . In these earlier works, high µmolar levels have been determined with reproducibilities in the range of about 2-5% for repeatability (n ≈ 10) and of about 4-12% for interday precision, respectively [7,8] . In order to enable the detection of drug molecules in the protein matrix (e.g.…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetic Investigations with Direct Injection of Plasma Samples: Possible Savings Using Capillary Electrophoresis (CE)

Kunkel¹,

Wätzig²

1999

Arch. Pharm. Pharm. Med. Chem.

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Capillary electrophoresis (CE) is often regarded as a separation technique of choice because of its high selectivity and its cost advantages compared to LC. RSD% of 0.5% have become standard for quality control assays. Using CE, sample pretreatment can often be significantly reduced, leading to notable savings of labor and reagent costs. Moreover, errors from sample pretreatment steps are avoided. A number of pharmaceuticals (e.g. acetaminophen, salicylic acid, sulfamethoxazole, theophylline, tolbutamide, and trimethoprim) have been determined in human plasma on underivatized fused silica capillaries by MEKC without sample pretreatment, the total analysis time being only 10 min. An sodium dodecyl sulfate‐containing borate buffer (60 mM with 200 mM SDS) at pH 10 has been used. Between runs, proteins adsorbed to the capillary wall are removed by a rinsing regimen consisting of SDS buffer and either acetonitrile (e.g. 50% v/v) or isopropanol (e.g. 10% v/v). Other rinsing approaches are discussed (salts, enzyme containing solutions, organic solvents, sodium hydroxide, hydrofluoric acid). The separation system is tested in a concentra‐tion range between 10 ng/mL and 100 μg/mL, the detection limit being about 5 ng/mL. The sensitivity has been substantially improved compared to preceding work using field‐amplified injection mechanisms and efficient computer algorithms that take advantage of multiwavelength detection. Correlations between the limit of quantitation (LOQ), the limit of detection (LOD) and the signal/noise ratio are discussed. A day‐to‐day precision for relative peak areas of 1 to 2% relsdv (n > 40) has been reached in the upper concentration range. Thus, not only drug monitoring but also pharmacokinetic investigations from blood plasma have become possible without further sample pretreatment.

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“…The high efficiency and resolution potential, relatively short analytical time, low instrumental costs and small sample volume make CE an alternative to HPLC for the determination of drugs in biological fluids [30,31] and the separation of metabolite mixtures [32,33]. A series of methods based on CE with ECL detection of tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy) 3 21 ), wherein a chemiluminescence reaction is initiated from reagents in the vicinity of the working electrode surface when potential is applied, have been developed for tertiary amines and their derivatives [22,[27][28][29][34][35][36][37].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of ethamsylate, tramadol and lidocaine in human urine by capillary electrophoresis with electrochemiluminescence detection

2006

Electrophoresis

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Ethamsylate, tramadol and lidocaine, partly excreted by the kidney, are generally used as hemostatic, analgesic and local anesthetic in surgery. We developed a simple and sensitive method for their simultaneous monitoring in human urine based on CE coupled with electrochemiluminescence detection by end-column mode. Under optimized conditions the proposed method yielded linear ranges from 5.0 x 10(-8) to 5.0 x 10(-5), 1.0 x 10(-7) to 1.0 x 10(-4) and 1.0 x 10(-7) to 1.0 x 10(-4) M with LODs of 8.0 x 10(-9) M (36 amol), 1.6 x 10(-8) M (72 amol) and 1.0 x 10(-8) M (45 amol) (S/N = 3) for ethamsylate, tramadol and lidocaine, respectively. The RSD for their simultaneous detection at 1.0 x 10(-6) M was 2.1, 2.8 and 3.2% (n = 7), respectively. For practical application an extraction step with ethyl acetate at pH 11 was performed to eliminate the influence of the sample ionic strength. The recoveries of ethamsylate, tramadol and lidocaine at different levels in human urine were between 87 and 95%. This method was used for simultaneous detection of ethamsylate, tramadol and lidocaine in clinic urine samples from two medicated patients. It was valuable in clinical and biochemical laboratories for monitoring these drugs for various purposes.

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Capillary electrophoresis in clinical and forensic analysis

Cited by 55 publications

References 111 publications

Molecular Genetics and its Applications in Forensic Sciences

Molecular Genetics and its Applications in Forensic Sciences

Pharmacokinetic Investigations with Direct Injection of Plasma Samples: Possible Savings Using Capillary Electrophoresis (CE)

Simultaneous determination of ethamsylate, tramadol and lidocaine in human urine by capillary electrophoresis with electrochemiluminescence detection

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