Capillary electrophoretic determination of amino acids with indirect absorbance detection

Lee, Y.-H.; Lin, Tsung‐I

doi:10.1016/0021-9673(94)80079-0

Cited by 71 publications

(28 citation statements)

References 16 publications

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“…This may well reflect differences in the mobility of the test BGE and the mobility of all the amino acids since the mobilities of amino acids are in the range of 1.0 to 4.0. (x 10 -4 cm 2 V-is -1) [15]. This was evident as peak deformation for amino acids with mobilities greater or less than that of the BGE.…”

Section: Characteristics Of the Bgesmentioning

confidence: 93%

“…The mobility of various BGEs was determined in 20 mM phosphate buffer at pH 11.0 [ 15]. Mobility was determined in a solution containing all BGEs at 0.1 mM each in deionized water and 0.05 % (v/v) benzyl alcohol as a neutral marker.…”

Section: Electrophoretic Determinationmentioning

confidence: 99%

“…Bruin et al [ 19] reported the use of salicylate as a BGE at pH 11.0 for the indirect UV detection of 7 amino acids in CZE. Lee et al [15] investigated several BGEs for CZE separation of amino acids with indirect UV detection, their results indicated that p-aminosalicylic and 4-(N,N-dimethyl amino)-benzoic acid with cyclodextrine additives [20] were the most suitable BGEs. More recently, Chen et al [21 ] reported the use of salicylate and benzoate as the BGEs for indirect UV detection of 14-18 amino acids, and its application to the determination of amino acids in urine samples.…”

Section: Introductionmentioning

confidence: 98%

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Separation of amino acids in plant tissue extracts by capillary zone electrophoresis with indirect UV detection using aromatic carboxylates as background electrolytes

2000

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Section: Characteristics Of the Bgesmentioning

confidence: 93%

Section: Electrophoretic Determinationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Separation of amino acids in plant tissue extracts by capillary zone electrophoresis with indirect UV detection using aromatic carboxylates as background electrolytes

2000

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“…Divalent cations as additives have been used to reduce EOF and improve resolution in previous work [4]. We investigated the effect of magnesium ions on the separation of 11 amino acids in the crowded region of Fig.…”

Section: Determination At Alkaline Ph Valuesmentioning

confidence: 99%

High‐voltage contactless conductivity detection of underivatized amino acids in capillary electrophoresis

2003

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High-voltage contactless conductivity detection of underivatized amino acids in both acidic and basic media is demonstrated. The suitability of different acidic buffer solutions at pH values of about 2.5 was investigated with 12 amino acids. Lactic acid as background electrolyte gave the best results in terms of detection limits for arginine, lysine and histidine, which were approximately 2 x 10(-7), 3 x 10(-7) and 4 x 10(-7) M, respectively. However, the sensitivity for other species was not quite as good and the detection limits in the order of 0.5-1 x 10 (-5) M. The use of basic conditions at pH 10-11 generally led to more stable baselines and more consistent sensitivities. A range of 20 amino acids was investigated with alkaline buffers and detection limits were typically about 10(-6) M. Urine and beer samples were analyzed. Nine and eleven amino acids could be identified, respectively.

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“…Although the separation time is short (maximum 20 min), the LOD ranged from 1350 to 0.59 mg/mL, being better with coated capillary only for Thr, Gly, Tyr, and Val. A more simple method was analyzed by Lee and Lin [19]. They studied nine BGEs for the capillary electrophoretic analysis of a mixture of 20 amino acids with indirect absorbance detection.…”

mentioning

confidence: 99%

Development of capillary electrophoresis methods for quantitative determination of taurine in vehicle system and biological media

et al. 2006

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CE methods have been developed for the determination of taurine in pharmaceutical formulation (microemulsion) and in biological media such as sweat. The CE system with end-column pulsed amperometric detection has been found to be an interesting method in comparison with UV and fluorescence detection for its simplicity and rapidity. A gold-disk electrode of 100 mm diameter was used as the working electrode. The effects of a field decoupler at the end of the capillary, separation voltage, injection and pressure times were investigated. A detection limit of 4 x 10(-5) mol/L was reached using integrated pulsed amperometric detection, a method successfully applied to taurine analysis of the biological samples such as sweat. For taurine analysis of oil-in-water microemulsion, fluorescence detector was the favored method, the detection limit of which was 4 x 10(-11) mol/L.

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Capillary electrophoretic determination of amino acids with indirect absorbance detection

Cited by 71 publications

References 16 publications

Separation of amino acids in plant tissue extracts by capillary zone electrophoresis with indirect UV detection using aromatic carboxylates as background electrolytes

Separation of amino acids in plant tissue extracts by capillary zone electrophoresis with indirect UV detection using aromatic carboxylates as background electrolytes

High‐voltage contactless conductivity detection of underivatized amino acids in capillary electrophoresis

Development of capillary electrophoresis methods for quantitative determination of taurine in vehicle system and biological media

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