Capillary Tube Based Molecular Diagnostic Test for Naked Eye Detection of Antibiotic Resistant Bacteria

Abstract

Antibiotic resistance bacteria (ARB) are a rising threat to public health. To prevent the emergence of antibiotic resistance strains of bacteria, selectively prescribing antibiotics is of the utmost importance. Under infection control in hospitals, rapid and accurate diagnosis of antibiotic resistant bacteria is highly critical. This study shows a new diagnostic platform for naked‐eye detection of antibiotics resistant bacteria using a capillary tube based optical detection method. For specific detection of target ARB, DNA hydrogel formation by isothermal amplification of complementary target (DhITACT) system is incorporated with magnetic beads (MBs). To facilitate naked‐eye optical detection, a molecular probe is immobilized on the surface of MBs. After selectively capturing target to the probe, generation of long DNA strands is achieved through isothermal rolling circle amplification (RCA). Physical entanglement of amplified DNA products triggers rapid interparticle aggregation among MBs. Using this phenomenon, a capillary tube‐based naked eye detection test is developed. This system offers facile optical detection of ARB with minimal procedures to support its wide use in a clinical set‐up.

Fabrication and characterisation of large area, uniform, and controllable surface relief patterns in photopolymer material

“…The effect of surface roughness and its major role in the adhesion of bacteria to the surface has been clearly demonstrated in recent years [8][9]. On the other hand, novel optical and photonic methods for detection of small number of bacteria, in contrast to the traditional microbiology methods where many bacteria are grown and examined, have been recently developed [1,5]. The main advantages of optical methods are immunity to external electromagnetic interferences, high sensitivity, parallel non-contact detection.…”

An open-space microfluidic chip with fluid walls for online detection of VEGF via rolling circle amplification

“…Based on this strategy, various cells could maintain regular growth. In addition, with the assistance of the RCA reaction for signal enlargement, [36][37][38] the approach enabled online detection of VEGF with good specicity and excellent linearity in the low concentration range. In particular, when cells were stimulated by deferoxamine (DFO), a drug for mimicking the hypoxic tumor microenvironment, 39 VEGF secreted by tumor cells would emerge with increasing tendency.…”