Capon comb growth-promoting substances (“male-hormones”) in human urine of males and females of varying ages

Dingemanse, E.; Borchardt, Helene; Laqueur, Ernst

doi:10.1042/bj0310500

Cited by 70 publications

(10 citation statements)

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“…In all determinations this level remained within the range of 40 to 100 I.U. which is the range for normal men as determined by several groups of workers (11,12,3). After the first few days castration symptoms remained absent throughout the course of treatment.…”

Section: Resultssupporting

confidence: 64%

“…Before androgenic administration, the level of output of male hormone in 4 urine assays covering 7 days, taken from a period of weeks, showed a daily average of 13.5 I.U. This is far below the levels of androgen excretion of normal adult men (11,12,3). Still further evidence that if any abdominal testis were present it did not secrete large amounts of androgens was the negative clinical response obtained with 16 injections of large amounts of anterior pituitary-like substance, 100 rat units (R.U.)…”

Section: Methods Materials and Subjectsmentioning

confidence: 77%

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Urinary Excretion of Androgenic Substances After Intramuscular and Oral Administration of Testosterone Propionate to Humans 1

Dorfman¹,

Hamilton²

1939

J. Clin. Invest.

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Section: Resultssupporting

confidence: 64%

Section: Methods Materials and Subjectsmentioning

confidence: 77%

Urinary Excretion of Androgenic Substances After Intramuscular and Oral Administration of Testosterone Propionate to Humans 1

Dorfman¹,

Hamilton²

1939

J. Clin. Invest.

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“…Since this paper was written an important contribution by Dingemanse, Borchardt and Laqueur (24) has appeared in which the usual male hormone values for the urine of normal men up to the age of 40 are given as 40 to 50 international units per liter. Aged men excreted from 5 to 40 units per liter.…”

Section: Addendummentioning

confidence: 99%

The Daily Urinary Excretion of Estrogenic and Androgenic Substances by Normal Men and Women 1

Gallagher¹,

Peterson²,

Dorfman³

et al. 1937

J. Clin. Invest.

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It is well established that normal men and women excrete estrogenic and androgenic substances in the urine. Qualitatively speaking, it is also well established that at least a part of the androgenic activity in men's urine is due to androsterone and dehydro-androsterone (1, 2, 3), but There is somewhat better agreement on the daily excretion of estrogenic activity in women, but practically no data on the excretion in men. Siebke (6) first reported 0 to 180 mouse units per liter of women's urine throughout the cycle. From studies on eight normal women he concluded that there is a low excretion previous to and during menstruation. Later Siebke (11) reported 10 to 140 mouse units of estrogenic activity per liter of urine in women with a distinct rise between the 10th to the 15th day of the cycle. Frank (12) reported 20 to 320 mouse units per day with peaks at 12 to 15 days after the onset of menstruation and 4 to 8 days before the next bleeding. Biihler (8) reported 10 to 60 mouse units per day in normal men. Gustavson and Green (13) and Smith and Smith (14) also report two peaks in the excretion of estrogenic activity in women during the menstrual cycle. The former find 0 to 50 rat units per day with peaks at the 9th to 14th day and the 14th to 21st day after menstruation. Smith and Smith (14) record the peaks at 12 to 17 days after menstruation and during menstruation.In this paper we present our methods of extraction and assay in detail and the results on four normal men and four normal women for 27 to 45 consecutive days. The male urine was collected 695

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“…Kemp and Pedersen-Bjergaard [1933] employed this method, and more recently Borchardt, Dingemanse, and Laqueur [1934] published an improved version of the method in which an adequate amount of acid was used ; the method was applied to the extrac¬ tion of androgens [Dingemanse, Borchardt, and Laqueur, 1937], and the superiority of their technique was supported by later studies [Dingemanse and Laqueur, 1938]. EXTRACTION OF STEROIDS FROM URINE 91 Dr. K. Pedersen-Bjergaard kindly informed us, during this work, of the details of his current method, in which carbon tetrachloride was used as the extracting solvent instead of benzene.…”

Section: Conditions Of Hydrolysis Of Urine By Acid Under Varying Condmentioning

confidence: 99%

“…A fuller systematic examination of variations in conditions of extraction, with this provisional method as a starting-point, and a comparison with others based on different principles, seemed to be called for in the light of our own experience, and of the claims made for methods published from other laboratories [cf. particularly Gallagher, Koch, and Dorfman, 1935 ;Peterson, Gallagher, and Koch, 1937 ;Dingemanse, Borchardt, and Laqueur, 1937 ;Dingemanse and Laqueur, 1938]. In addition it was hoped to obtain further light on the conditions for extraction of oestrogens from urine.…”

mentioning

confidence: 99%

Methods of Extracting Compounds Related to the Steroid Hormones From Human Urine

Callow¹,

Callow²,

Emmens³

et al. 1939

Journal of Endocrinology

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At the beginning of biochemical work on male hormones it was assumed that the androgenic material which could be extracted from normal male urine was`the male hormone', or 'the testicular hormone'. On this supposition, it was hoped that the biological assay of urine extracts would give an index of the activity of androgenic substances in the human body, possibly referable to the level of testicular secretion. It is for this reason that the separation of the maximal androgenic activity as measured by capon comb-growth has been taken as the criterion of a satisfactory extraction in the methods developed by systematic work in various laboratories. It has since been realized, however, that androgenic activity is not specific to one substance, and that at least two related compounds with androgenic activity are present in the mixture of neutral compounds which can be prepared from urine. Further, these two compounds, androsterone and ¿ratts-dehydroandrosterone (¿35-androsten-3(/3)-ol-17-one), which can be isolated from the urine of women as well as from the urine of men [Callow and Callow, 1938], have not been found in tissue, whilst the only androgen isolated as yet from testis tissue, testosterone, has not been found in urine.

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Capon comb growth-promoting substances (“male-hormones”) in human urine of males and females of varying ages

Cited by 70 publications

References 1 publication

Urinary Excretion of Androgenic Substances After Intramuscular and Oral Administration of Testosterone Propionate to Humans 1

Urinary Excretion of Androgenic Substances After Intramuscular and Oral Administration of Testosterone Propionate to Humans 1

The Daily Urinary Excretion of Estrogenic and Androgenic Substances by Normal Men and Women 1

Methods of Extracting Compounds Related to the Steroid Hormones From Human Urine

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