Carbapenem Resistance in Clonally Distinct Clinical Strains of<i>Vibrio fluvialis</i>Isolated from Diarrheal Samples

Chowdhury, Goutam; Pazhani, Gururaja P.; Sarkar, Anirban; Rajendran, K.; Mukhopadhyay, Asish K.; Bhattacharya, Mihir; Ghosh, Amit; Ramamurthy, Thandavarayan

doi:10.3201/eid2210.151612

Cited by 21 publications

(22 citation statements)

References 37 publications

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“…The carbapenemase NDM-1 has been found mainly in Enterobacteriaceae, such as Escherichia coli and Klebsiella pneumoniae (2). However, recent reports have described the existence of bla NDM genes in Vibrio parahaemolyticus, Vibrio fluvialis, and Vibrio cholerae from clinical or environmental samples (3,4). In this study, a Vibrio sp.…”

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confidence: 78%

Identification and Characterization of IncA/C Conjugative, bla _NDM-1 -Bearing Plasmid in Vibrio alginolyticus of Food Origin

Zheng

et al. 2018

Antimicrob Agents Chemother

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confidence: 78%

Identification and Characterization of IncA/C Conjugative, bla _NDM-1 -Bearing Plasmid in Vibrio alginolyticus of Food Origin

Zheng

et al. 2018

Antimicrob Agents Chemother

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“…It harbors a genome containing several MGEs such as plasmids, superintegrons and insertion sequences (ISs) carrying cargo genes, particularly resistance and virulence genes [21]. Tetracyclines, aminoglycosides, third generation cephalosporins and fluoroquinolones are generally used as antibiotic therapy in human vibriosis outbreaks [13,22] but, several cases of multidrug-resistant (MDR) vibrios are reported in literature even to the last-resort antibiotics used in human medicine such as carbapenems [22][23][24][25][26][27].…”

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confidence: 99%

Resistome, Mobilome and Virulome Analysis of Shewanella algae and Vibrio spp. Strains Isolated in Italian Aquaculture Centers

et al. 2020

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Antimicrobial resistance is a major public health concern restricted not only to healthcare settings but also to veterinary and environmental ones. In this study, we analyzed, by whole genome sequencing (WGS) the resistome, mobilome and virulome of 12 multidrug-resistant (MDR) marine strains belonging to Shewanellaceae and Vibrionaceae families collected at aquaculture centers in Italy. The results evidenced the presence of several resistance mechanisms including enzyme and efflux pump systems conferring resistance to beta-lactams, quinolones, tetracyclines, macrolides, polymyxins, chloramphenicol, fosfomycin, erythromycin, detergents and heavy metals. Mobilome analysis did not find circular elements but class I integrons, integrative and conjugative element (ICE) associated modules, prophages and different insertion sequence (IS) family transposases. These mobile genetic elements (MGEs) are usually present in other aquatic bacteria but also in Enterobacteriaceae suggesting their transferability among autochthonous and allochthonous bacteria of the resilient microbiota. Regarding the presence of virulence factors, hemolytic activity was detected both in the Shewanella algae and in Vibrio spp. strains. To conclude, these data indicate the role as a reservoir of resistance and virulence genes in the environment of the aquatic microbiota present in the examined Italian fish farms that potentially might be transferred to bacteria of medical interest.

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“…has been detected and isolated from environmental and seafood samples ( Walsh et al, 2011 ; Mandal et al, 2012 ; Gu et al, 2014 ; Bier et al, 2015 ). Recently, in Kolkata, NDM-1 producing Vibrio fluvialis strains has been isolated from diarrheal fecal samples from patients ( Chowdhury et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Discovery on Antibiotic Resistance Patterns of Vibrio parahaemolyticus in Selangor Reveals Carbapenemase Producing Vibrio parahaemolyticus in Marine and Freshwater Fish

et al. 2018

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Vibrio parahaemolyticus, a Gram-negative halophilic bacterium is often associated with fish and fishery products, thus causing gastroenteritis in humans upon ingestion of contaminated food. V. parahaemolyticus has become a globally well-known pathogen with yearly reported cases in many countries. This study aimed to discover the antibiotic resistance patterns of V. parahaemolyticus as well as detect Carbapenem resistant isolates from marine and freshwater fish in Selangor. A total of 240 freshwater and marine fish samples collected from wet market and supermarket in Selangor were tested for the presence of V. parahaemolyticus. All the fish samples were determined positive for V. parahaemolyticus using conventional microbiological culture-based method. The toxR gene were detected via polymerase chain reaction (PCR) in 165/240 (69%) isolates. The two-virulence factor of V. parahaemolyticus, thermostable direct hemolysin (tdh) and TDH-related hemolysin (trh) was screened via PCR. As such, four isolates were trh+and none were tdh+. Majority of the isolates presented high resistance to ampicillin (88%), amikacin (64%), and kanamycin (50%). In addition, this study identified 19-imipenem resistant isolates isolated from freshwater and marine fish samples. Further analysis of these 19-imipenem resistant isolates revealed that the resistance toward imipenem was plasmid mediated after plasmid curing assay. The multiple antibiotics resistance index was >0.2 for 70% of the isolates. In summary, the results confirm the presence of V. parahaemolyticus in freshwater and marine fish samples in Selangor, Malaysia. To our best knowledge, this is the first report discovering the antibiotic resistant patterns and Carbapenem-resistant isolates of V. parahaemolyticus isolated from marine and freshwater fish samples in Selangor.

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Carbapenem Resistance in Clonally Distinct Clinical Strains ofVibrio fluvialisIsolated from Diarrheal Samples

Abstract: These strains might acquire the blaNDM-1 gene without exposure to antimicrobial drugs.

Cited by 21 publications

References 37 publications

Identification and Characterization of IncA/C Conjugative, bla _NDM-1 -Bearing Plasmid in Vibrio alginolyticus of Food Origin

Identification and Characterization of IncA/C Conjugative, bla _NDM-1 -Bearing Plasmid in Vibrio alginolyticus of Food Origin

Resistome, Mobilome and Virulome Analysis of Shewanella algae and Vibrio spp. Strains Isolated in Italian Aquaculture Centers

Discovery on Antibiotic Resistance Patterns of Vibrio parahaemolyticus in Selangor Reveals Carbapenemase Producing Vibrio parahaemolyticus in Marine and Freshwater Fish

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Carbapenem Resistance in Clonally Distinct Clinical Strains ofVibrio fluvialisIsolated from Diarrheal Samples

Abstract: These strains might acquire the blaNDM-1 gene without exposure to antimicrobial drugs.

Cited by 21 publications

References 37 publications

Identification and Characterization of IncA/C Conjugative, bla NDM-1 -Bearing Plasmid in Vibrio alginolyticus of Food Origin

Identification and Characterization of IncA/C Conjugative, bla NDM-1 -Bearing Plasmid in Vibrio alginolyticus of Food Origin

Resistome, Mobilome and Virulome Analysis of Shewanella algae and Vibrio spp. Strains Isolated in Italian Aquaculture Centers

Discovery on Antibiotic Resistance Patterns of Vibrio parahaemolyticus in Selangor Reveals Carbapenemase Producing Vibrio parahaemolyticus in Marine and Freshwater Fish

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Identification and Characterization of IncA/C Conjugative, bla _NDM-1 -Bearing Plasmid in Vibrio alginolyticus of Food Origin

Identification and Characterization of IncA/C Conjugative, bla _NDM-1 -Bearing Plasmid in Vibrio alginolyticus of Food Origin