Carbohydrate metabolism is a determinant for the host specificity of baculovirus infections

Tsai, Chih-Hsuan; Chuang, Yi-Chi; Lu, Yun-Heng; Lin, C.H.; Tang, Cheng-Kang; Wei, Sung-Chan; Wu, Yueh‐Lung

doi:10.1016/j.isci.2021.103648

Cited by 8 publications

(5 citation statements)

References 50 publications

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“…At the same time, by annotating the KEGG pathway of DEGs, we found that DEGs were mainly related to amino acid metabolism and carbohydrate metabolism in the metabolic category (Figure 2b). Studies have shown that the amino acid content in the haemolymph of silkworms infected with BmNPV will change significantly (Feng et al, 2021), and the content of carbohydrates (such as trehalose and glycogen) will also change (Tsai et al, 2022), which is consistent with our results.…”

Section: Enrichment Analysis Of Degs In Rna-seqsupporting

confidence: 92%

Enhanced locomotor behaviour is mediated by activation of tyrosine hydroxylase in the silkworm brain

Wang

Xie

et al. 2023

Insect Molecular Biology

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Animal behaviour regulation is a complex process involving many factors, and the nervous system is an essential factor in this process. In many species, pathogens can alter host behaviour by affecting the host's nervous system. An interesting example is that the silkworm shows enhanced locomotor behaviour after being infected with the nucleopolyhedrosis virus. In this study, we analysed the transcriptome of the silkworm brain at different time points after infection and found that various genes related to behaviour regulation changed after infection. In‐depth analysis showed that the tyrosine hydroxylase gene might be a key candidate gene, and the content of dopamine, its downstream metabolite, increased significantly in the brain of silkworms infected with the virus. After the injection of tyrosine hydroxylase inhibitor into the infected silkworm, the dopamine content in the silkworm brain decreased and the locomotor behaviour caused by the virus was blocked successfully. These results confirm that tyrosine hydroxylase is involved in regulating enhanced locomotor behaviour after virus infection in silkworms. Furthermore, the tyrosine hydroxylase gene was specifically overexpressed in the brain of the silkworm, and the transgenic silkworm was enhanced in locomotor behaviour and foraging behaviour. These results suggest that the tyrosine hydroxylase gene plays a vital role in regulating insect behaviour.

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Section: Enrichment Analysis Of Degs In Rna-seqsupporting

confidence: 92%

Enhanced locomotor behaviour is mediated by activation of tyrosine hydroxylase in the silkworm brain

Wang

Xie

et al. 2023

Insect Molecular Biology

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“…The approach may also apply to other baculoviruses which have a wide host range. The baculovirus AcMNPV is known to infect larvae of more than 39 species of Lepidoptera insects, including Plutella xylostella, Trichoplusia ni, and Spodoptera frugiperda [51,52]. Many variants of AcMNPV have been isolated from Lepidoptera insects besides A. californica, such as Plutella xylostella multiple nucleopolyhedrovirus isolate CL3 (PlxyMNPV-CL3) isolated from P. xylostella [53], RoMNPV isolated from Rachiplusia ou [54], and AfMNPV isolated from A. falcifera.…”

Section: Discussionmentioning

confidence: 99%

Virus-host coevolutionary analyses of an Alphabaculovirus with a wide host range

Yang,

Peng,

Lei

et al. 2024

Journal of General Virology

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Baculoviruses are highly host specific, and their host range is usually restricted to a single or a few closely related insect species, except for few virus species, e.g. Alphabaculovirus aucalifonicae and Alphabaculovirus mabrassicae. In this study, two new alphabaculovirus isolates were isolated from the larvae of Mamestra brassicae and Mythimna separata, which were named as Mamestra brassicae multiple nucleopolyhedrovirus isolate QD (MbMNPV-QD) and Mythimna separata multiple nucleopolyhedrovirus isolate Hb (MyseMNPV-Hb), respectively. The Kimura two-parameter values based on the concatenated 38 core genes of baculovirus revealed that MbMNPV (isolates QD/CHb1/K1/CTa), MyseMNPV-Hb, Helicoverpa armigera multiple nucleopolyhedrovirus (HearMNPV) and Mamestra configurata nucleopolyhedrovirus B (MacoNPV-B) were different isolates of a same virus species. A phylogenetic tree of baculoviruses and nudiviruses constructed from their 20 homologous gene sequences, and that of their isolated hosts constructed from 13 protein-coding genes of the insect mitochondrial genomes, were used to analyse the coevolution of baculoviruses with their isolated hosts. The results showed that M. brassicae was the most likely ancestral host of these virus isolates, included MbMNPV isolates, MyseMNPV-Hb, HearMNPV, and MacoNPV-B. Therefore, we concluded that these virus isolates belong to the existing virus species – Alphabaculovirus mabrassicae with M. brassicae as their ancestral host.

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“…As the center of intermediates and energy metabolism, the TCA cycle occupies a critical position in various metabolic pathways [ 38 ]. A previous study showed that AcMNPV infection upregulated the expression of metabolic enzyme genes ( cs , aco2, and idh3 ) in the host TCA cycle [ 10 ]. Therefore, we examined the transcription levels of these three genes in BmN cells after BmNPV infection.…”

Section: Discussionmentioning

confidence: 99%

“…It is also the final metabolic pathway for glucose, protein, and lipid [ 9 ]. The study of energy metabolism in Autographa californica multiple nucleopolyhedroviruses (AcMNPV) showed that viral infection enhances the consumption of glucose in the host, which then enters the glycolytic pathway and TCA cycle [ 10 ]. In addition, AcMNPV infection drives a large amount of ATP synthesis by activating the expression of TCA cycle genes [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Deacetylation of ACO2 Is Essential for Inhibiting Bombyx mori Nucleopolyhedrovirus Propagation

Hu,

You,

et al. 2023

Viruses

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Bombyx mori nucleopolyhedrovirus (BmNPV) is a specific pathogen of Bombyx mori that can significantly impede agricultural development. Accumulating evidence indicates that the viral proliferation in the host requires an ample supply of energy. However, the correlative reports of baculovirus are deficient, especially on the acetylation modification of tricarboxylic acid cycle (TCA cycle) metabolic enzymes. Our recent quantitative analysis of protein acetylome revealed that mitochondrial aconitase (ACO2) could be modified by (de)acetylation at lysine 56 (K56) during the BmNPV infection; however, the underlying mechanism is yet unknown. In order to understand this regulatory mechanism, the modification site K56 was mutated to arginine (Lys56Arg; K56R) to mimic deacetylated lysine. The results showed that mimic deacetylated mitochondrial ACO2 restricted enzymatic activity. Although the ATP production was enhanced after viral infection, K56 deacetylation of ACO2 suppressed BmN cellular ATP levels and mitochondrial membrane potential by affecting citrate synthase and isocitrate dehydrogenase activities compared with wild-type ACO2. Furthermore, the deacetylation of exogenous ACO2 lowered BmNPV replication and generation of progeny viruses. In summary, our study on ACO2 revealed the potential mechanism underlying WT ACO2 promotes the proliferation of BmNPV and K56 deacetylation of ACO2 eliminates this promotional effect, which might provide novel insights for developing antiviral strategies.

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Carbohydrate metabolism is a determinant for the host specificity of baculovirus infections

Cited by 8 publications

References 50 publications

Enhanced locomotor behaviour is mediated by activation of tyrosine hydroxylase in the silkworm brain

Enhanced locomotor behaviour is mediated by activation of tyrosine hydroxylase in the silkworm brain

Virus-host coevolutionary analyses of an Alphabaculovirus with a wide host range

Deacetylation of ACO2 Is Essential for Inhibiting Bombyx mori Nucleopolyhedrovirus Propagation

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