Carbon-to-nitrogen ratios for Agaricus brasiliensis on the axenic method

Zaghi, Lienine Luiz; Linde, Giani Andréa; Colauto, Nelson Barros

doi:10.4025/actasciagron.v32i1.5086

Cited by 3 publications

(2 citation statements)

References 19 publications

(8 reference statements)

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“…The differences in yield amongst substrates may be due to differences in substrate's porosity and water holding capacity. [8] asserts that the productivity of the different substrates is dependent on the water holding capacity as well as porosity of the substrates which allows for efficient respiration of mycelia and easy access for enzymes to the nutrients present in them, thus resulting in better mycelial development. The differences in yield may also be due to the presence of less complex carbohydrates and other growth factors.…”

Section: Resultsmentioning

confidence: 99%

Effect of Carbon-Nitrogen Ratios of Lignocellulosic Substrates on the Yield of Mushroom (Pleurotus pulmonarius)

Osunde¹,

Olayinka²,

Fashina³

et al. 2019

OALib

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Mushrooms are fungi that are unique in the recycling of plant biomass through decomposition in the process of which they also serve as food for man. These functions are carried out through the extracellular digestion of plant biomass with a consortium of enzymes. This study examined the effect of carbon-nitrogen ratios (C/N) of three lignocellulosic substrates, namely, corncob (C/N 120:1), sawdust (C/N 325:1) and waste paper (C/N 400:1) on the yield of Pleurotus pulmonarius. The experimental design was factorial laid out in Randomized Complete Block Design (RCBD). Tissue culture was made on Potato Dextrose Agar (PDA) which was transferred to boiled sorghum grains for spawn making. The substrates were pasteurized and then inoculated with P. pulmonarius. Substrates were pasteurized (immersed in hot water for 3 hours) and inoculated with P. pulmonarius. Proximate analysis of the fruiting bodies was done to determine crude protein, crude fat, moisture content, dry matter, ash, crude fiber and carbohydrate. Carbon-nitrogen (C/N) ratio of the substrates was also determined. Corncob has C/N ratio of 120:1. The yield performance of corncob was the highest for weight (86 ± 0.01 g), length of stipe (3.55 ± 0.04 cm) and diameter of pileus (7.3 ± 0.07 cm). Means separation by least significant difference (LSD) showed that the yield parameters for corncob were statistically different from other substrates used.The proximate analysis of dried P. pulmonarius showed that corncob had a protein content of 17.37% ± 0.80% and moisture content of 0.69% ± 0.01% respectively, while sawdust and corncob had crude fibre contents of 1.79 ± 0.02 and 1.79 ± 0.01 respectively. Conclusively, having an idea of the carbon-nitrogen ratio of the substrate to be used for mushroom cultivation, manipulating the nutritional composition of mushrooms can be enhanced by enriching the growth substrates/media.

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Section: Resultsmentioning

confidence: 99%

Effect of Carbon-Nitrogen Ratios of Lignocellulosic Substrates on the Yield of Mushroom (Pleurotus pulmonarius)

Osunde¹,

Olayinka²,

Fashina³

et al. 2019

OALib

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“…Research on Agaricus species has focused on enhancing the yield and sporophore quality, including the usage of alternative substrates and techniques for successful fructification [35][36][37][38]. For example, the soybean fiber structure, the protein and polysaccharide composition, along with a greater N content resulting in a substrate formulation ratio of C/N = 11 (N = 4.25%), offered a more suitable substrate for A. subrufescens mycelial growth in a variety of carbon to nitrogen ratios from 11 to 248 [39], while the dry matter and carbohydrate content of A. bisporus mushrooms increased during cultivation when using casing materials having higher levels of proteins and hence nitrogen content [40]. Regarding Pleurotus species, although around 200 species of this genus have been discovered, only a few have been exploited in food technology [32,41], notably the species Pleurotus ostreatus (oyster mushroom) and Pleurotus eryngii (king oyster), which can grow on hard timbers in forests across a wide temperature range.…”

Section: Introductionmentioning

confidence: 99%

Examining the Impact of Substrate Composition on the Biochemical Properties and Antioxidant Activity of Pleurotus and Agaricus Mushrooms

Diamantopoulou,

Fourtaka,

Melanouri

et al. 2023

Fermentation

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The composition of the substrate is one of the most critical factors influencing the quality as well as the nutritional value and bioactive content of mushrooms. Therefore, the effects of various substrates, such as barley and oat straw (BOS), beech wood shavings (BWS), coffee residue (CR), rice bark (RB) and wheat straw (WS, control substrate), on the biochemical properties (lipid, protein, polysaccharide, glucan, ash, and mineral content, fatty acids and tocopherols composition), total phenolic compounds and antioxidant activity of Pleurotus mushrooms, P. ostreatus (strains AMRL 144, 150) and P. eryngii (strains AMRL 166, 173-6), cultivated in ‘bag-logs’, was examined. Proximate analysis of A. bisporus and A. subrufescens grown on two different composts (C/N ratios of 10 and 13) was conducted, too. The whole carposomes, pilei and stipes were analyzed. Results showed that BOS, RB, BWSs and CR improved the antioxidant activity of Pleurotus species and their nutritional characteristics. Both pilei and stipes were rich in polysaccharides (27.51–67.37 and 22.46–39.08%, w/w, for Pleurotus and Agaricus spp., respectively), lipids (0.74–8.70 and 5.80–9.92%, w/w), proteins (6.52–37.04 and 25.40–44.26, w/w, for Pleurotus and Agaricus spp., respectively) and total phenolic compounds (10.41–70.67 and 7.85–16.89 mg gallic acid equivalent/g for Pleurotus and Agaricus spp., respectively), while they contained important quantities of unsaturated FAs of nutritional and medicinal importance. Pilei were richer in proteins, total phenolic compounds and enhanced antioxidant activity and reducing power than stipes, whereas stipes were richer in IPSs and glucans compared to the corresponding pilei. Thus, mushroom cultivation could upgrade rejected agro-industrial residues and wastes to new uses as substrates for the production of mushrooms with specific nutritional and medicinal attributes.

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Utilization of Paper-Cone Water Cups as an Alternative Lignocellulose Waste Substrate in Pleurotus ostreatus Production

Suwanno

Ayae

Suwanno

2019

Walailak J Sci & Tech

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This study examined the utilization of paper-cone water cups as an alternative substrate for oyster mushroom (Pleurotus ostreatus) production. The research was conducted by using bioconversion technology and a profitable method for converting lignocellulosic residue from municipal solid waste into protein-rich biomass, thereby reducing waste paper and enhancing environmental quality. The most suitable substrate for mycelial growth was waste paper from paper-cone water cups (WPC) combined with rubber wood sawdust (RWS) at a 75%:25% dry basis ratio. The substrate mixture was adjusted to a moisture content of 70 %, and the C/N ratio was fixed at 20:1 by the addition of urea and supplementation with 8 % rice bran. Spawn running used 10 % seed inoculum. The mushrooms were cultivated on 500 g of substrate in polyvinyl chloride boxes (405 cm3) and incubated at 25 °C in the dark with the relative humidity maintained at 70 - 80 %. The fastest spawn running (mycelia development) occurred at 5th days, with pin head formation at 9th days and fruiting body formation at 12th days.The highest yield recorded was 26.59g/100g.Under these conditions, the potential lignocellulosic waste conversion (biological efficiency) was recorded as 88.64 %, and the protein content of P. ostreatus was 35.75 % after 12 days of cultivation.

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Carbon-to-nitrogen ratios for Agaricus brasiliensis on the axenic method

Cited by 3 publications

References 19 publications

Effect of Carbon-Nitrogen Ratios of Lignocellulosic Substrates on the Yield of Mushroom (Pleurotus pulmonarius)

Effect of Carbon-Nitrogen Ratios of Lignocellulosic Substrates on the Yield of Mushroom (Pleurotus pulmonarius)

Examining the Impact of Substrate Composition on the Biochemical Properties and Antioxidant Activity of Pleurotus and Agaricus Mushrooms

Utilization of Paper-Cone Water Cups as an Alternative Lignocellulose Waste Substrate in Pleurotus ostreatus Production

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