Cardamonin suppresses melanogenesis by inhibition of Wnt/β-catenin signaling

Cho, Munju; Ryu, Min-Jung; Jeong, Yongsu; Chung, Young‐Hwa; Kim, Dong-Eun; Cho, Ho-Song; Kang, Sung Soo; Han, Jong‐Sub; Chang, Min-Youl; Lee, Cheon-Koo; Jin, Muhyun; Kim, Ho-Jeoung; Oh, Sangtaek

doi:10.1016/j.bbrc.2009.09.124

Cited by 67 publications

(52 citation statements)

References 39 publications

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“…The data showed that Wnt3a significantly increased tyrosinase activity and melanin synthesis in melan-a cells. In support of our findings, previous studies have reported that inhibition of Wnt/β-catenin signaling strongly inhibited melanin synthesis (20,39).…”

Section: Discussionsupporting

confidence: 92%

Wnt3a inhibits proliferation but promotes melanogenesis of melan-a cells

Guo

Yang

Deng

et al. 2012

International Journal of Molecular Medicine

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Abstract. Melanocytes are pigment-producing cells responsible for coloration of skin and hair. Although the importance of Wnt3a in melanocyte development has been well recognized, the role of Wnt3a in mature melanocytes has not been elucidated. This study was conducted to further explore the effects of Wnt3a on melanocyte proliferation and melanogenesis, and to elucidate the possible mechanisms involved. We infected melan-a cells with AdWnt3a to serve as the production source of the Wnt3a protein. MTT assay, 5-bromodeoxyuridine incorporation assay and flow cytometric analysis showed that Wnt3a inhibited the proliferation of melan-a cells and this was associated with decrease of cells in the S phase and increase of cells in the G 1 phase. Melanin content and tyrosinase activity assay revealed that Wnt3a significantly promoted melanogenesis of melan-a cells. Furthermore, western blot analysis showed that Wnt3a upregulated the expression of microphthalmia-associated transcription factor and its downstream target genes, tyrosinase and tyrosinase-related protein 1 in melan-a cells. Collectively, our results suggest that Wnt3a plays an important role in melanocyte homeostasis.

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Section: Discussionsupporting

confidence: 92%

Wnt3a inhibits proliferation but promotes melanogenesis of melan-a cells

Guo

Yang

Deng

et al. 2012

International Journal of Molecular Medicine

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“…To evaluate whether ␤-catenin signaling is required for the increase in , we supplemented ConT with cardamonin (10 mol/l). Cardamonin that was previously described to stabilize ␤-catenin in its degradation complex (12) significantly attenuated the ConT-induced increase in Cx43 protein expression and (ConT ϩ carda: t 4h : 1.52 Ϯ 0.20 cm/s, n ϭ 7; ConT: t 4h : 2.52 Ϯ 0.20 cm/s, n ϭ 8; P Ͻ 0.05) (Fig. 4, A and B).…”

Section: Hl-1 Cells and Mscs Establish Intercellular Coupling Via Gapmentioning

confidence: 79%

Mesenchymal stem cells improve cardiac conduction by upregulation of connexin 43 through paracrine signaling

Mureli

Gans

Bare

et al. 2013

American Journal of Physiology-Heart and Circulatory Physiology

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Mesenchymal stem cells (MSCs) were shown to improve cell survival and alleviate cardiac arrhythmias when transplanted into cardiac tissue; however, little is known about the mechanism by which MSCs modify the electrophysiological properties of cardiac tissue. We aimed to distinguish the influence of cell-cell coupling between myocytes and MSCs from that of MSC-derived paracrine factors on the spontaneous activity and conduction velocity (θ) of multicellular cardiomyocyte preparations. HL-1 cells were plated on microelectrode arrays and their spontaneous activity and θ was determined from field potential recordings. In heterocellular cultures of MSCs and HL-1 cells the beating frequency was attenuated (t(0h): 2.26 ± 0.18 Hz; t(4h): 1.98 ± 0.26 Hz; P < 0.01) concomitant to the intercellular coupling between MSCs and cardiomyocytes. In HL-1 monolayers supplemented with MSC conditioned media (ConM) or tyrode (ConT) θ significantly increased in a time-dependent manner (ConT: t(0h): 2.4 cm/s ± 0.2; t(4h): 3.1 ± 0.4 cm/s), whereas the beating frequency remained constant. Connexin (Cx)43 mRNA and protein expression levels also increased after ConM or ConT treatment over the same time period. Enhanced low-density lipoprotein receptor-related protein 6 (LRP6) phosphorylation after ConT treatment implicates the Wnt signaling pathway. Suppression of Wnt secretion from MSCs (IWP-2; 5 μmol/l) reduced the efficacy of ConT to induce phospho-LRP6 and to increase θ. Inhibition of β-catenin (cardamonin; 10 μmol/l) or GSK3-α/β (LiCl; 5 mmol/l) also suppressed changes in θ, further supporting the hypothesis that MSC-mediated Cx43 upregulation occurs in part through secreted Wnt ligands and activation of the canonical Wnt signaling pathway.

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“…Wnt signalling is essential for organogenesis of the skin and its appendages, such as hairs, feathers, and scales [30]. Moreover, Wnt/β-catenin signalling plays important roles in melanocyte development and differentiation [31-33]. Chicken HOXC8 is reported to be expressed in dorsal dermal and epidermal cells during the first stage of feather morphogenesis [34].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Profile Analysis of Mechanisms of Black and White Plumage Determination in Black-Bone Chicken

Wang

Liao

et al. 2018

Cell Physiol Biochem

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Background/Aims: Melanin is a major and ubiquitous component of plumage colouration, and patterns of melanin pigmentation in birds are extremely varied. However, the molecular mechanism of pigmentation in avian plumage is still largely unknown. Methods: To elucidate the molecular mechanisms involved in the formation of black and white plumage, this study takes advantage of high-throughput sequencing technology to compare differences in the transcriptome between black and white chicken feather bulbs. In total, we constructed six cDNA libraries from black (Group B) and white (Group W) feather bulbs in the dorsal plumage of Muchuan black-boned chickens. Results: A comparison between Groups B and W revealed 61 differentially expressed genes, with 47 displaying higher, and 14 displaying lower, levels of expression in white feather bulbs. Our results revealed a set of candidate genes and two potential metabolic pathways involved in black-bone chicken plumage melanogenesis. These include four homeobox genes (HOXB9, HOXC8, HOXA9, and HOXC 9), two glutathione (GSH) metabolism-related genes (CHAC1 and GPX3), and the transforming growth factor beta (TGF-β) signalling pathway. Two known genes, TYR and MITF, were also shown to play a role in melanin formation. Conclusion: our data provide a valuable resource for discovering genes important in plumage melanin formation and will help further elucidate the molecular mechanisms for black and white plumage.

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Cardamonin suppresses melanogenesis by inhibition of Wnt/β-catenin signaling

Cited by 67 publications

References 39 publications

Wnt3a inhibits proliferation but promotes melanogenesis of melan-a cells

Wnt3a inhibits proliferation but promotes melanogenesis of melan-a cells

Mesenchymal stem cells improve cardiac conduction by upregulation of connexin 43 through paracrine signaling

Transcriptome Profile Analysis of Mechanisms of Black and White Plumage Determination in Black-Bone Chicken

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