Cardiogel: A Nano-Matrix Scaffold with Potential Application in Cardiac Regeneration Using Mesenchymal Stem Cells

Santhakumar, Rajalakshmi; Vidyasekar, Prasanna; Verma, Rama Shanker

doi:10.1371/journal.pone.0114697

Cited by 35 publications

(26 citation statements)

References 67 publications

(80 reference statements)

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“…Natural multicomponent ECMs derived from tissues have also been used in 3D to influence cell differentiation toward cardiomyocytes. Cardiogel, which is derived from cardiac fibroblasts, has been shown to control differentiation of bone marrow-derived stem cells toward a cardiomyocyte phenotype [38]. The components of cardiogel have been characterized and contain laminins, fibronectin, Col I, Col III, and a variety of proteoglycans [39].…”

Section: In Vitro Control Systems To Evaluate Cell-cecm Interplay At mentioning

confidence: 99%

Cardiac Extracellular Matrix Modification as a Therapeutic Approach

Hall

Ogle

2018

Advances in Experimental Medicine and Biology

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The cardiac extracellular matrix (cECM) is comprised of proteins and polysaccharides secreted by cardiac cell types, which provide structural and biochemical support to cardiovascular tissue. The roles of cECM proteins and the associated family of cell surface receptor, integrins, have been explored in vivo via the generation of knockout experimental animal models. However, the complexity of tissues makes it difficult to isolate the effects of individual cECM proteins on a particular cell process or disease state. The desire to further dissect the role of cECM has led to the development of a variety of in vitro model systems, which are now being used not only for basic studies but also for testing drug efficacy and toxicity and for generating therapeutic scaffolds. These systems began with 2D coatings of cECM derived from tissue and have developed to include recombinant ECM proteins, ECM fragments, and ECM mimics. Most recently 3D model systems have emerged, made possible by several developing technologies including, and most notably, 3D bioprinting. This chapter will attempt to track the evolution of our understanding of the relationship between cECM and cell behavior from in vivo model to in vitro control systems. We end the chapter with a summary of how basic studies such as these have informed the use of cECM as a direct therapy.

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Section: In Vitro Control Systems To Evaluate Cell-cecm Interplay At mentioning

confidence: 99%

Cardiac Extracellular Matrix Modification as a Therapeutic Approach

Hall

Ogle

2018

Advances in Experimental Medicine and Biology

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“…Compared to natural scaffolds which are derived from destroyed ECM, decellularized ECM‐derived scaffolds are advantageous to preserve the complex ECM architecture and thus to maintain cell‐matrix interactions, which are uneasy to achieve by synthetic polymer scaffolds . For example, Verma and co‐workers developed several ECM‐derived scaffolds, termed as ‘cardiogel’, to repair infarcted myocardial tissues . Cardiogel promoted proliferation, adhesion and migration of BMSCs while aiding cardiomyogenic differentiation and angiogenesis .…”

Section: Type Of Polymers and Design Strategiesmentioning

confidence: 99%

“…[26,27] For example, Verma and co-workers developed several ECM-derived scaffolds, termed as 'cardiogel', to repair infarcted myocardial tissues. [28][29][30] Cardiogel promoted proliferation, adhesion and migration of BMSCs while aiding cardiomyogenic differentiation and angiogenesis. [30] ADSCs embedded in decellularized adipose tissue (DAT)-derived ECM scaffold accelerated in vivo fat regeneration through enhanced adipogenesis and angiogenesis.…”

Section: Biodegradable Scaffold To Promote Angiogenesismentioning

confidence: 99%

“…[28][29][30] Cardiogel promoted proliferation, adhesion and migration of BMSCs while aiding cardiomyogenic differentiation and angiogenesis. [30] ADSCs embedded in decellularized adipose tissue (DAT)-derived ECM scaffold accelerated in vivo fat regeneration through enhanced adipogenesis and angiogenesis. [31] The DAT retained ECM components including collagen I, IV, laminin [32] and angiogenic growth factors, [33] exhibited similar mechanical properties to native human fat, [34] thereby providing a highly supportive microenvironment for adipose regeneration.…”

Section: Biodegradable Scaffold To Promote Angiogenesismentioning

confidence: 99%

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Design of Polymeric Culture Substrates to Promote Proangiogenic Potential of Stem Cells

Kwon

Wang

Kang

et al. 2017

Macromolecular Bioscience

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Stem cells are a promising cell source for regenerative medicine due to their differentiation and self-renewal capacities. In the field of regenerative medicine and tissue engineering, a variety of biomedical technologies have been tested to improve proangiogenic activities of stem cells. However, their therapeutic effect is found to be limited in the clinic because of cell loss, senescence, and insufficient therapeutic activities. To address this type of issue, advanced techniques for biomaterial synthesis and fabrication have been approached to mimic proangiogenic microenvironment and to direct proangiogenic activities. This review highlights the types of polymers and design strategies that have been studied to promote proangiogenic activities of stem cells. In particular, scaffolds, hydrogels, and surface topographies, as well as insight into their underlying mechanisms to improve proangiogenic activities are the focuses. The strategy to promote angiogenic activities of hMSCs by controlling substrate repellency is introduced, and the future direction is proposed.

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“…Previous study indicated that certain kinds whole organ ACM could support BMSCs differentiating into mature cells and expressing functional markers [26][27][28][29]. Furthermore, this type of induced cells had potential applications in regenerative therapy and tissue repair [30].…”

Section: Introductionmentioning

confidence: 99%

Pancreatic line Cell Differentiation of bone marrow Mesenchymal Stromal Cells in Acellular Pancreatic Scaffolds

Zhao

Wang

2020

Preprint

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Background: To evaluate the potential differentiation ability of bone mesenchymal stromal cells(BMSCs) to pancreatic line Cells on rat acellular pancreatic bioscaffold (APB). Methods: Fresh pancreata from 20 Adult Sprague Dawley rats (between 6 and 7 weeks old) were soaked and perfused using Easy-Load Digital Drive peristaltic pumps. After BMSCs were isolated and identified, they were dynamic cultured on the APB and static cultured in tissue culture flask(TCF). Based on whether the differentiation was induced by the growth factors (GF) in the culture system, our study was divided into 4 groups: BMSCs cultured in TCF without any GF(TCF-GF(-)), BMSCs cultured in TCF with GF(TCF-GF(+)), BMSCs cultured on APB without GF(APB-GF(-)), BMSCs cultured on APB with GF (APB-GF(+)).The cytological behavior such as the proliferation and differentiation of BMSCs in all the above kinds of culture system with or without GF were assessed by morphological observation, flow cytometry, ELASA analysis, qRT-PCR assay and western blot analysis. Results: 4ml/min was the most appropriate flow rate for the dynamic culture of BMSCs. Under culture conditions, BMSCs populations could attach to and proliferate within the APB. APB could promote the proliferation and viability of BMSCs significantly better in dynamic culture with optimal flow rate 4ml/min, when compared to the static culture system. Also, the proliferation rate of BMSCs in the APB groups were significantly increased compared to TCF system. During the pancreatic line cell differentiation process, APB could induce BMSCs into pancreatic-like cells which expressed markers such as pancreatic duodenal homeodomain containing transcription factor (PDX-1) and pancreatic exocrine transcription factor (PTF-1) higher at mRNA levels compared to TCF system. In contrast, the marker Oct4 (octamer-binding transcription factor 4) was expressed at a lower level in APB group. For the pancreatic functional cytoketatins including α-Amylase(α-Amy), cytokeratin 7(CK7), fetal liver kinase-1 (Flk-1),and C-peptide, they were all expressed at higher level in APB group than in the TCF group. And metabolic enzymes secretion such as amylase and insulin were promoted significantly in APB system. By scanning electron microscope(SEM) and transmission electron microscopy(TEM), the ultrastructure of BMSCs in the APB group could further demonstrated the morphological characteristics of pancreatic-like cells. In addition, in both the dynamic and static system, GF could significantly facilitate the function of proliferation, differentiation and cell engraftment . Conclusion: Together our data show the capacity of APB , 3D pancreatic biomatrix, promoting BMSCs differentiate toward pancreatic line phenotypes, and the considerable potential of using these cells for pancreatic cell therapies and tissue engineering.

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Cardiogel: A Nano-Matrix Scaffold with Potential Application in Cardiac Regeneration Using Mesenchymal Stem Cells

Cited by 35 publications

References 67 publications

Cardiac Extracellular Matrix Modification as a Therapeutic Approach

Cardiac Extracellular Matrix Modification as a Therapeutic Approach

Design of Polymeric Culture Substrates to Promote Proangiogenic Potential of Stem Cells

Pancreatic line Cell Differentiation of bone marrow Mesenchymal Stromal Cells in Acellular Pancreatic Scaffolds

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