Cardioprotective Effect of Modified Peroxiredoxins in Retrograde Perfusion of Isolated Rat Heart under Conditions of Oxidative Stress

Karaduleva, E. V.; Мубаракшина, Э. К.; Шарапов, М. Г.; Volkova, A.; Pimenov, O. Yu.; Равин, В. К.; Kokoz, Yu. M.; Новоселов, В. И.

doi:10.1007/s10517-016-3237-1

Cited by 19 publications

(9 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It also has phospholipase activity and can therefore either reduce the oxidized sn2 fatty acyl group of phospholipids (peroxidase activity) or hydrolyze the sn2 ester bond of phospholipids (phospholipase activity). It plays a role in phospholipid homeostasis and in cell protection against oxidative stress by detoxifying peroxides [81]. Mitochondrial superoxide dismutase decreased in fructose-fed rats, and this result is consistent with the findings of Lappalainen et al showing that SOD2 decreased in the kidney of STZ-induced diabetic rats [82].…”

Section: Resultssupporting

confidence: 87%

Prediabetes Induced by Fructose-Enriched Diet Influences Cardiac Lipidome and Proteome and Leads to Deterioration of Cardiac Function prior to the Development of Excessive Oxidative Stress and Cell Damage

Szűcs

Sója

Péter

et al. 2019

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Prediabetes is a condition affecting more than 35% of the population. In some forms, excessive carbohydrate intake (primarily refined sugar) plays a prominent role. Prediabetes is a symptomless, mostly unrecognized disease which increases cardiovascular risk. In our work, we examined the effect of a fructose-enriched diet on cardiac function and lipidome as well as proteome of cardiac muscle. Male Wistar rats were divided into two groups. The control group received a normal diet while the fructose-fed group received 60% fructose-supplemented chow for 24 weeks. Fasting blood glucose measurement and oral glucose tolerance test (OGTT) showed slightly but significantly elevated values due to fructose feeding indicating development of a prediabetic condition. Both echocardiography and isolated working heart perfusion performed at the end of the feeding protocol demonstrated diastolic cardiac dysfunction in the fructose-fed group. Mass spectrometry-based, high-performance lipidomic and proteomic analyses were executed from cardiac tissue. The lipidomic analysis revealed complex rearrangement of the whole lipidome with special emphasis on defects in cardiolipin remodeling. The proteomic analysis showed significant changes in 75 cardiac proteins due to fructose feeding including mitochondria-, apoptosis-, and oxidative stress-related proteins. Nevertheless, just very weak or no signs of apoptosis induction and oxidative stress were detected in the hearts of fructose-fed rats. Our results suggest that fructose feeding induces marked alterations in the cardiac lipidome, especially in cardiolipin remodeling, which leads to mitochondrial dysfunction and impaired cardiac function. However, at the same time, several adaptive responses are induced at the proteome level in order to maintain a homeostatic balance. These findings demonstrate that even very early stages of prediabetes can impair cardiac function and can result in significant changes in the lipidome and proteome of the heart prior to the development of excessive oxidative stress and cell damage.

show abstract

Section: Resultssupporting

confidence: 87%

Prediabetes Induced by Fructose-Enriched Diet Influences Cardiac Lipidome and Proteome and Leads to Deterioration of Cardiac Function prior to the Development of Excessive Oxidative Stress and Cell Damage

Szűcs

Sója

Péter

et al. 2019

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

show abstract

“…The decrease in the level of exogenous Prx1 and Prx2 in the bloodstream may be due to their distribution in the tissues. For instance, Prx6 and its modified forms have been shown to distribute under the vascular endothelium, at the border with the basement membrane [ 50 ]. It should be mentioned that in addition to the reduction of the Prx1 and Prx2 concentration in the blood of animals after administration, a decrease in the peroxidase activity of exogenous enzymes over time was also observed.…”

Section: Resultsmentioning

confidence: 99%

Comparative Study of Protective Action of Exogenous 2-Cys Peroxiredoxins (Prx1 and Prx2) Under Renal Ischemia-Reperfusion Injury

et al. 2020

View full text Add to dashboard Cite

The pathogenesis of ischemia-reperfusion (I/R) injuries is based on oxidative stress caused by a sharp increase in the concentration of free radicals, reactive oxygen species (ROS) and secondary products of free radical oxidation of biological macromolecules during reperfusion. Application of exogenous antioxidants lowers the level of ROS in the affected tissues, suppresses or adjusts the course of oxidative stress, thereby substantially reducing the severity of I/R injury. We believe that the use of antioxidant enzymes may be the most promising line of effort since they possess higher efficiency than low molecular weight antioxidants. Among antioxidant enzymes, of great interest are peroxiredoxins (Prx1–6) which reduce a wide range of organic and inorganic peroxide substrates. In an animal model of bilateral I/R injury of kidneys (using histological, biochemical, and molecular biological methods) it was shown that intravenous administration of recombinant typical 2-Cys peroxiredoxins (Prx1 and Prx2) effectively reduces the severity of I/R damage, contributing to the normalization of the structural and functional state of the kidneys and an almost 2-fold increase in the survival of experimental animals. The use of recombinant Prx1 or Prx2 can be an efficient approach for the prevention and treatment of renal I/R injury.

show abstract

“…Соэкспрессией соединенных генов удалось получить трехфункциональный белковый конъюгат с активностью Mn-СОД, КАТ и способностью вхождения в клетки [45]. Рекомбинантный химерный белок, обладающий пероксидазной и СОД-активностью, проявил кардиопротекторные свойства на изолированном сердце крысы с модельным окислительным стрессом, вызванным пероксидом водорода [46,47]. Кардиопротекторный эффект подтверждался нормализацией частоты сердечных сокращений, поддержанием контрактильной функции миокарда и предупреждением губительного действия окислительного стресса.…”

Section: Av Maksimenko Approximation Of New Generation Pharmacologiunclassified

Approximation of new generation pharmacological enzyme researches to clinical practice

Maksimenko¹

2018

Kardio. vestn.

View full text Add to dashboard Cite

Исследования модифицированных ферментных производных значительно расширяют арсенал лечебных средств врача. Биомедицинское изучение показало достоверную эффективность действия модифицированных ферментных производных in vivo и перспективность их терапевтического применения. Сформировалось несколько направлений исследований действия модифицированных биокатализаторов против различных поражений, в том числе и кардиологического профиля. Можно выделить исследования производных антитело-лекарство, конструирование полиферментных наноансамблей вне-и внутриклеточного действия, разработку модифицированных ферментов. Эти исследования могут стать успешным прорывом в лечении различных поражений благодаря совместным усилиям работников науки и медицины.

show abstract

Cardioprotective Effect of Modified Peroxiredoxins in Retrograde Perfusion of Isolated Rat Heart under Conditions of Oxidative Stress

Cited by 19 publications

References 9 publications

Prediabetes Induced by Fructose-Enriched Diet Influences Cardiac Lipidome and Proteome and Leads to Deterioration of Cardiac Function prior to the Development of Excessive Oxidative Stress and Cell Damage

Prediabetes Induced by Fructose-Enriched Diet Influences Cardiac Lipidome and Proteome and Leads to Deterioration of Cardiac Function prior to the Development of Excessive Oxidative Stress and Cell Damage

Comparative Study of Protective Action of Exogenous 2-Cys Peroxiredoxins (Prx1 and Prx2) Under Renal Ischemia-Reperfusion Injury

Approximation of new generation pharmacological enzyme researches to clinical practice

Contact Info

Product

Resources

About