Carnitine Levels in Iron-Deficient Rat Pups

Bartholmey, Sandra J.; Sherman, Adria Rothman

doi:10.1093/jn/115.1.138

Cited by 38 publications

(10 citation statements)

References 25 publications

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“…Infants are at greatest risk because their principal and often only source of nutrition is milk, which is notoriously low in Fe (Picciano & Guthrie, 1976;Picciano et al 1981). In the rat, maternal Fe deficiency results in a decrease in the concentration of milk Fe (Bartholmey & Sherman, 1985). However, the possible effects of maternal Fe deficiency on milk production and concentrations of major milk constituents have not been examined.…”

Section: Impact Of Maternal Iron Deficiency On Quality and Quantity Omentioning

confidence: 99%

Impact of maternal iron deficiency on quality and quantity of milk ingested by neonatal rats

1988

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1. The effect of maternal iron deficiency on milk composition and consumption by sucking rats was investigated.2. Dams (n42) were fed on semi-purified diets with either 8 (Fe-) or 250 (Fe+) mg Fe as ferrous sulphate/kg throughout gestation and lactation. Total milk intake was determined at days 7, 12 and 17 of lactation from the rate of disappearance of3H2O from the total body water pool of pups. Measurements of milk constituents and Fe status of animals also were made.3. Feeding the Fe- diet led to the development of anaemia in dams and pups and to growth retardation of sucking pups.4. Concentrations of total lipid and Fe in milk from Fe- dams were significantly lower than those from Fe + dams. Mean milk intakes (ml/d) of Fe-deficient pups were 21 and 28% less than intakes of Fe-sufficient pups on days 12 and 17 respectively. However, when expressed per kg body-weight, mean milk intakes were similar between groups on days 17 and 12 and increased by 47% in the Fe-deficient group on day 17 of lactation.5. It is concluded that maternal Fe deficiency affects the quality of milk ingested by neonatal rats. However, Fe-deficient pups are at least partially able to compensate for reduced milk energy and nutrient contents by increasing intake in late lactation.

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Section: Impact Of Maternal Iron Deficiency On Quality and Quantity Omentioning

confidence: 99%

Impact of maternal iron deficiency on quality and quantity of milk ingested by neonatal rats

1988

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“…The drug rhEpo is known to stimulate erythropoiesis in rats. The suckling rat has previously been used to model absolute iron deficiency by feeding pregnant and lactating dams a severely irondeficient diet [7,8], but no model has thus far been reported for functional iron deficiency, nor the border between functional iron deficiency and absolute iron deficiency. As a foundation for our studies, characterization of the hematological parameters of our neonatal rat population was necessary prior to examination of the effect of rhEpo therapy on these same parameters.…”

Section: Introductionmentioning

confidence: 99%

Iron-Deficient Erythropoiesis in Neonatal Rats

Dubuque

Dvořák²,

Woodward³

et al. 2002

Neonatology

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Anemia in premature infants is extremely common. Precise quantitation of iron status and determination of iron incorporation into erythrocytes are important in monitoring therapy for anemia in premature infants, especially when treating with recombinant human erythropoietin (rhEPO). However, the traditional indices of the iron status have limited usefulness in this population. The goal of the current work is to develop an experimental animal model system that addresses the clinical issue relating to quantitation of iron delivery to erythrocytes. We first determined normal hematological values for nontreated, dam-suckled Sprague-Dawley rats by measuring markers of erythropoiesis and iron status during the first 12 postnatal days (PND). The experimental group of rats were administered parenteral rhEpo (430 IU·kg^–1 · day^–1) for 8 days (from PND 4 until PND 12) in the absence (rhEpo^–Fe) or presence (rhEpo^+Fe) of oral iron supplementation (6 mg·kg^–1·day^–1). Rat pups receiving oral iron only (control^+Fe) and pups that were sham fed with the orogastric tube (control^–Fe) were included as controls. Hematological parameters were measured in blood and bone marrow. In a pattern similar to that seen in premature infants during the first 2 months of life, the levels of these hematopoietic markers were dynamic and changed during the first 12 PND. After challenging experimental animals with subcutaneous rhEpo, evidence of iron-deficient erythropoiesis was seen in the rhEpo^–Fe group. Red blood cell levels and absolute reticulocyte counts were higher in both groups receiving rhEpo as compared with the controls. However, the rhEpo^–Fe group experienced a lower hemoglobin level, a lower mean red cell volume, a greater red cell distribution width, and a higher zinc protoporphyrin/heme (ratio than the rhEpo^+Fe group. The neonatal rat is an excellent model of iron-deficient erythropoiesis and will be useful in designing future mechanistic studies examining the interplay between iron and erythropoiesis in the anemic, iron-challenged premature neonate.

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“…Similarly, Chen et al (2016) found that 3.3 μM Fe concentration up-regulated the mRNA expressions of lipolytic enzymatic genes on day 28. In rats, Fe deficiency reduced β-oxidation of fatty acids, allowing the fatty acids to be diverted towards TG synthesis (Bartholmey and Sherman, 1985). Davis et al (2012) also reported that Fe deficiency reduced gene expression related to hepatic β-oxidation.…”

Section: Discussionmentioning

confidence: 99%