Carob fibre compounds modulate parameters of cell growth differently in human HT29 colon adenocarcinoma cells than in LT97 colon adenoma cells

Klenow, Stefanie; Glei, Michael; Haber, Bernd; Owen, Robert W.; Pool‐Zobel, Beatrice L.

doi:10.1016/j.fct.2007.09.003

Cited by 47 publications

(37 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Fiber, cyclitols, polyphenols and tannins have mainly attracted scientific attention. These groups of bioactive compounds have been linked with the health-promoting effects of carob in different therapeutic areas, including anti-cancer, anti-diabetes, anti-diarrheal and anti-hyperlipidemia [29,30,31,32,33,34]. These findings have rendered carob fruit an excellent ingredient for the development of functional food and herbal supplements.…”

Section: Introductionmentioning

confidence: 99%

Functional Components of Carob Fruit: Linking the Chemical and Biological Space

Goulas

Stylos

Chatziathanasiadou

et al. 2016

IJMS

132

135

View full text Add to dashboard Cite

The contribution of natural products to the drug-discovery pipeline has been remarkable since they have served as a rich source for drug development and discovery. Natural products have adapted, during the course of evolution, optimum chemical scaffolds against a wide variety of diseases, including cancer and diabetes. Advances in high-throughput screening assays, assisted by the continuous development on the instrumentation’s capabilities and omics, have resulted in charting a large chemical and biological space of drug-like compounds, originating from natural sources. Herein, we attempt to integrate the information on the chemical composition and the associated biological impact of carob fruit in regards to human health. The beneficial and health-promoting effects of carob along with the clinical trials and the drug formulations derived from carob’s natural components are presented in this review.

show abstract

Section: Introductionmentioning

confidence: 99%

Functional Components of Carob Fruit: Linking the Chemical and Biological Space

Goulas

Stylos

Chatziathanasiadou

et al. 2016

IJMS

132

135

View full text Add to dashboard Cite

show abstract

“…Nonetheless, we assume that oxidation of apple phenolic compounds still occurs since we found evidence for the oxidation in the brownish color change as described by Kader et al [57]. H 2 O 2 formed by this oxidation processes is not detectable due to reduced stability in this particular medium [39]. In addition, results with AE[A] in HT29 cell culture medium (DMEM with 10% FCS), revealed an extensive formation of H 2 O 2 (240 l 8 lM H 2 O 2 with 510 lg/mL AE after 24 h, data not shown).…”

Section: Discussionmentioning

confidence: 63%

“…Peroxide production or rather H 2 O 2 formation in the cellfree MCDB culture medium in the presence of AE or SPM was analyzed using the ferric-xylenol orange assay (FOX) method [38,39]. …”

Section: Determination Of H 2 O 2 Formationmentioning

confidence: 99%

Impact of apple polyphenols on GSTT2 gene expression, subsequent protection of DNA and modulation of proliferation using LT97 human colon adenoma cells

Miene¹,

Klenow²,

Veeriah³

et al. 2009

Molecular Nutrition Food Res

View full text Add to dashboard Cite

Apple extract (AE) enhances expression of glutathione S-transferases (e.g., GSTT2) in human colon cells (LT97). Therefore, aim of the present study was to identify functional consequences of GSTT2 induction by AE and to determine the relation of AE effects to isolated compounds. Polyphenol composition of AE was analyzed. LT97 cells were treated with AE or synthetic polyphenol mixture (SPM) under conditions that induced GSTT2, and challenged with GSTT2-2 substrate cumene hydroperoxide (CumOOH) to determine DNA damage using comet assay. Modulation of GSTT2 expression (real-time PCR) was reassessed, and the influence on cell proliferation and pro-oxidative potential of AE and SPM were assessed to understand additional mechanisms. Induction of GSTT2 by AE was accompanied by protection of LT97 cells from CumOOH-induced genotoxicity. Although SPM was unable to reflect AE-specific bioactivity related to GSTT2 modulation and anti-genotoxicity, inhibition of LT97 cell proliferation by SPM was comparable. Storage of AE caused changes in phenolic composition along with loss of activity regarding GSTT2 induction and amplified growth inhibition. At the applied concentrations, no H(2)O(2) formation was detectable with any of the substances. AE can protect against oxidatively induced DNA damage. Nevertheless, chemopreventive effects of AE strongly depend on the specific composition, which is modified by storage.

show abstract

“…Primarily contained in the seeds, its ingredients such as gallic acid and polyphenols showed markedly antiarteriosclerotic, antimicrobial, antioxidant, and antidepressant effects in recent studies [17,18].…”

Section: Plants and Drugs In Ancient Egyptian Medicinementioning

confidence: 99%

Back to the roots – dermatology in ancient Egyptian medicine

Hartmann

2016

J Deutsche Derma Gesell

View full text Add to dashboard Cite

SummaryAlthough ancient Greek and Roman medicine is generally considered the origin of European medicine, there is evidence in ancient Egyptian texts suggesting a precursor role of ancient Egyptian medicine in this regard. What did Greek and Roman physicians learn from their Egyptian counterparts? Of the medical papyri discovered to date, the largest and most significant -the Ebers papyrus and the Smith papyrus -originate from the beginning of the New Kingdom, however, they were -at least in part -already written during the Old Kingdom. Considering the times, the spectrum of diseases treated as well as the range of conservative and surgical treatment methods was truly astounding. Taking a medical history, performing a thorough manual examination, and assessing clinical findings constituted key components in establishing a diagnosis. Apart from hygienic aspects, skin and hair disorders, the treatment of acute and chronic wounds and injuries as well as cosmetic procedures took on an important role. Even back then, physicians sought to assess inflammatory processes with respect to their cardinal features, implement graded wound therapy, and treat diseases with allopathic drugs. The 'channel theory' prevalent at that time, in which the unimpeded flow of bodily fluids was considered a fundamental prerequisite for health, may likely be regarded as precursor of ancient Greek humoral pathology. The latter became the basis for the subsequently established theory of the four humors, and was thus essential for the entire field of medieval medicine.

show abstract

Carob fibre compounds modulate parameters of cell growth differently in human HT29 colon adenocarcinoma cells than in LT97 colon adenoma cells

Cited by 47 publications

References 52 publications

Functional Components of Carob Fruit: Linking the Chemical and Biological Space

Functional Components of Carob Fruit: Linking the Chemical and Biological Space

Impact of apple polyphenols on GSTT2 gene expression, subsequent protection of DNA and modulation of proliferation using LT97 human colon adenoma cells

Back to the roots – dermatology in ancient Egyptian medicine

Contact Info

Product

Resources

About