Caspase-3 and GFAP as early markers for apoptosis and astrogliosis in shRNA-induced hippocampal cytotoxicity

Günther, Anne; Luczak, Vincent; Abel, Ted; Baumann, Arnd

doi:10.1242/jeb.154583

Cited by 19 publications

(19 citation statements)

References 24 publications

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“…The pathway is dominated by a bcl-2 family members that regulate the release of cytochrome-c from the mitochondria then activate caspase protease that dismantle cells to make apoptosis formation and signal efficient phagocytosis of cell corspses. [1718] It is consistent with the previous study which showed that annonacin from Annonaceae family induces the apoptotic cell death in a caspase-3 related to the pathway on endometrial cancer cells. [19]…”

Section: Discussionsupporting

confidence: 90%

In vitro anticancer activity Annona squamosa extract nanoparticle on WiDr cells

Fadholly

Proboningrat

Iskandar

et al. 2019

J Adv Pharm Technol Res

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This study aimed to prepare Annona squamosa leaf extract-loaded chitosan nanoparticles (nano-ASLE) against human colon cancer (WiDr) cells. Nano-ASLE was made with ionic gelation method. Four concentrations of the nano-ASLE (50, 100, 200, and 400 μg/mL) in dimethyl sulfoxide were prepared on WiDr cells to determine the IC50 value using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Then, it was divided into three groups of concentration of IC50, 2IC50, and 4IC50 and continued with analysis of caspase-3 expression and cell cycle arrest. The results of particles size were obtained 535.1 nm and showed potent cytotoxicity with IC50 292.39 μg/mL. The expression of caspase-3 increased significantly and caused cell cycle arrest at the G2/M phase and induced apoptosis on WiDr cells. Further studies are needed to obtain the loading efficiency, release of drug concentration, and in vivo study of nano-ASLE to suppress WiDr cells.

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Section: Discussionsupporting

confidence: 90%

In vitro anticancer activity Annona squamosa extract nanoparticle on WiDr cells

Fadholly

Proboningrat

Iskandar

et al. 2019

J Adv Pharm Technol Res

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“…One of the apoptosis marker in the early stage is showed by caspase-3 expression. 17,18 In this study, other compounds of Annona squamosa like flavonoid, alkaloid and saponin triggered ROS production that induced apoptosis through actions at different signal transduction pathways such as cyclin-dependent kinases (CDKs), caspases, Bcl-2 family members, epidermal growth factor (EGF)/epidermal growth factor receptor (EGFR), phosphatidylinositol-3-kinase/Akt, MAPK and NF-κB, which may affect cellular function by modulating genes or phosphorylating proteins. 19 The result of caspase-3 expression in immunocytochemistry shows that the increasing doses followed by the increasing amount of caspase-3 expression.…”

Section: Acknowledgementmentioning

confidence: 93%

Apoptosis of HeLa Cells via Caspase-3 Expression Induced by Chitosan-Based Nanoparticles of Annona squamosa Leaf Extract: In vitro Study

Fadholly¹,

Ansori²,

Proboningrat³

et al. 2020

IJPER

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Background: Annona squamosa is reported has a significant cytotoxic activity in some cancer cells. Objectives: Thus, this study aim to investigate Annona squamosa leaf extract induced by chitosan nanoparticles (nano-ASLE) to enhance their biological activity as anticancer agent on HeLa cells. Methods: Nano-ASLE (50, 100, 200, 400 µg/mL in DMSO) given on HeLa cells to determined IC 50 value by MTT assay. Then, it was devided into three groups as follow IC 50 , 2IC 50 , 4IC 50 continued with analysis of caspase-3 expression. Results: The present study demonstrated that nano-ASLE can surpress HeLa cells proliferation with the IC 50 value of 344.48 µg/mL and rapid enhancement of caspase-3 activity has the mean score of 65.3 cell expression and the lowest score shows 45.3 cell expression. Conclusion: Nano-ASLE lead to HeLa cell death via the mitochondrial pathway on caspase-3 expression. In addition, the further studies are needed to obtain the loading efficiency, release of drug concentration and in vivo study of nano-ASLE to suppress HeLa cells.

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“…To gain further insight into the mechanism accompanying the loss of the CA1 pyramidal cell layer in sh2-treated mice, we used active (cleaved) caspase-3 (Caspase) and glial fibrillary acidic protein (GFAP) as indicators for the adverse tissue responses [26] (Figure 6). At 5 weeks post-injection, fluorescent signals of active caspase-3, a main component of apoptosis in eukaryotic cells [27], was very high, especially in the CA1 subfield of sh2-injected hippocampi compared to shScr-injected hippocampi (Figure 6, upper panel).…”

Section: Molecular and Immunological Analyses Of Hcn2 Knock-downmentioning

confidence: 99%

Loss of HCN2 in Dorsal Hippocampus of Young Adult Mice Induces Specific Apoptosis of the CA1 Pyramidal Neuron Layer

Deutsch

Stegmayr

Balfanz

et al. 2021

IJMS

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Neurons inevitably rely on a proper repertoire and distribution of membrane-bound ion‑conducting channels. Among these proteins, the family of hyperpolarization-activated and cyclic nucleotide-gated (HCN) channels possesses unique properties giving rise to the corresponding Ih-current that contributes to various aspects of neural signaling. In mammals, four genes (hcn1-4) encode subunits of HCN channels. These subunits can assemble as hetero- or homotetrameric ion-conducting channels. In order to elaborate on the specific role of the HCN2 subunit in shaping electrical properties of neurons, we applied an Adeno-associated virus (AAV)-mediated, RNAi-based knock-down strategy of hcn2 gene expression both in vitro and in vivo. Electrophysiological measurements showed that HCN2 subunit knock-down resulted in specific yet anticipated changes in Ih-current properties in primary hippocampal neurons and, in addition, corroborated that the HCN2 subunit participates in postsynaptic signal integration. To further address the role of the HCN2 subunit in vivo, we injected recombinant (r)AAVs into the dorsal hippocampus of young adult male mice. Behavioral and biochemical analyses were conducted to assess the contribution of HCN2-containing channels in shaping hippocampal network properties. Surprisingly, knock-down of hcn2 expression resulted in a severe degeneration of the CA1 pyramidal cell layer, which did not occur in mice injected with control rAAV constructs. This finding might pinpoint to a vital and yet unknown contribution of HCN2 channels in establishing or maintaining the proper function of CA1 pyramidal neurons of the dorsal hippocampus.

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Caspase-3 and GFAP as early markers for apoptosis and astrogliosis in shRNA-induced hippocampal cytotoxicity

Cited by 19 publications

References 24 publications

In vitro anticancer activity Annona squamosa extract nanoparticle on WiDr cells

In vitro anticancer activity Annona squamosa extract nanoparticle on WiDr cells

Apoptosis of HeLa Cells via Caspase-3 Expression Induced by Chitosan-Based Nanoparticles of Annona squamosa Leaf Extract: In vitro Study

Loss of HCN2 in Dorsal Hippocampus of Young Adult Mice Induces Specific Apoptosis of the CA1 Pyramidal Neuron Layer

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