Caspase-3 serves as an intracellular immune receptor specific for lipopolysaccharide in oyster Crassostrea gigas

Xu, Jialin; Jiang, Shuai; Li, Yiqun; Li, Meijia; Cheng, Qi; Zhao, Depeng; Yang, Bin; Jia, Zhihao; Wang, Lingling; Song, Linsheng

doi:10.1016/j.dci.2016.03.015

Cited by 56 publications

(40 citation statements)

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“…Caspase 3 belongs to a family of cysteine proteases called caspases and plays a crucial role in the immune system homeostasis by controlling the programmed cell death process. 17 The increasing expression of caspase 3 by feeding DF may induce jejunal cell apoptosis; these results disagree with that of a previous study that caspase 3 is downregulated in the colon of pigs receiving PF. 42 This phenomenon may be related to the effects of PF on caspase 3 gene expression in response to different intestinal segments, and the mechanisms might depend on NF-kB signaling pathway.…”

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confidence: 57%

Effects of dietary fiber on the antioxidant capacity, immune status, and antioxidant-relative signaling molecular gene expression in rat organs

Fang

Cao

et al. 2017

RSC Adv.

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This study was conducted to evaluate the effects of different fibers, such as dietary pea, sweet potato, and wheat bran fibers, on the antioxidant capacity, immune status, and antioxidant-related signaling molecules of rat organs. In a 30 day experiment, a total of 44 rats were randomly divided into four dietary groups. These rats were fed either with a control diet or three basal diets supplemented with 15% pea fiber, 15% sweet potato fiber, and 15% wheat bran fiber. Results are as follows: (1) different fibers, such as dietary pea, sweet potato, and wheat bran fibers, can effectively promote antioxidant defense by decreasing free radicals and increasing non-enzymatic and enzymatic antioxidant capacities in relevant organs (P < 0.05).(2) Pea, sweet potato, and wheat bran fiber significantly increase the mRNA levels of enzymatic antioxidant substances in relevant organs. Moreover, supplementation of these fibers remarkably increased the NF-E2-related nuclear factor 2, Kelch-like ECH-associated protein 1, and mammalian target of rapamycin in the spleen (P < 0.05). (3) These fibers also significantly upregulated the antiinflammatory mRNA level but downregulated the pro-inflammatory cytokine mRNA level in the jejunum, liver, and spleen. The mRNA level of transforming growth factor beta 1 also increased in rats with wheat bran fiber in different tissues (P < 0.05). In summary, pea fiber, sweet potato fiber, and wheat bran fiber supplements can improve the antioxidant capacity of plasma and relevant organs and regulate antioxidant-related signaling molecular expression in the jejunum, liver, and spleen of rats. Various fibers exerted different effects on immunity function in the jejunum, liver, and spleen by mediating the gene expression levels of anti-inflammatory and pro-inflammatory cytokines, TGF-b, and caspase 3.

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confidence: 57%

Effects of dietary fiber on the antioxidant capacity, immune status, and antioxidant-relative signaling molecular gene expression in rat organs

Fang

Cao

et al. 2017

RSC Adv.

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“…Apoptosis is an active form of programmed cell death that plays a vital role in the maintenance and development of organism homeostasis [8, 9, 26]. It allows cells to undergo a highly regulated form of death in response to various proapoptotic stimuli.…”

Section: Discussionmentioning

confidence: 99%

“…FACS analysis showed an increased proportion of apoptotic cells in CPS- and M. ovipneumoniae -treated cells compared to untreated cells, which was consistent with the results from Western blotting, from which the cleaved-caspase-3 and cleaved-PARP1 were increased in both CPS- and M. ovipneumoniae- treated cells. Of particular interest, the caspase-3, one of the most widely studied and the most key apoptosis effectors [9], was strikingly activated following a stimulation of CPS and M. ovipneumoniae cells, thereby suggesting that CPS- and M. ovipneumoniae- induced cell toxicity were at least in part related to an apoptotic progression. In order to determine the effects of ROS in regulating apoptosis, a ROS scavenger, NAC (N-Acetyl-L-cysteine), was used to further clarify the underlying mechanism.…”

Section: Discussionmentioning

confidence: 99%

“…The released cytochrome C subsequently leads to the release of apoptosis inducing factor (AIF), which in turn activates caspase cascades, finally resulting in cell apoptosis [33, 34]. In this signal cascade, caspases are key regulators in the apoptotic pathway, which generally consist of the upstream initiators, such as caspase-8, -9, and -10 and the downstream effectors such as caspase-3, -6, and -7 [9]. In line with this notion, we firstly confirmed that mitochondria were involved in CPS- and M. ovipneumoniae -induced cell apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…Apoptosis is an active form of programmed cell death that plays a crucial role in the development and maintenance of organism homeostasis by eliminating of damaged or redundant cells [7–9]. In this context, organisms can employ antioxidant defense system to counteract oxidative stress and further prevent oxidative damage [10].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Capsular Polysaccharide of Mycoplasma ovipneumoniae Induces Sheep Airway Epithelial Cell Apoptosis via ROS‐Dependent JNK/P38 MAPK Pathways

Jiang

Song

et al. 2017

Oxidative Medicine and Cellular Longevity

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In an attempt to better understand the pathogen-host interaction between invading Mycoplasma ovipneumoniae (M. ovipneumoniae) and sheep airway epithelial cells, biological effects and possible molecular mechanism of capsular polysaccharide of M. ovipneumoniae (CPS) in the induction of cell apoptosis were explored using sheep bronchial epithelial cells cultured in air-liquid interface (ALI). The CPS of M. ovipneumoniae was first isolated and purified. Results showed that CPS had a cytotoxic effect by disrupting the integrity of mitochondrial membrane, accompanied with an increase of reactive oxygen species and decrease of mitochondrial membrane potential (ΔΨm). Of importance, the CPS exhibited an ability to induce caspase-dependent cell apoptosis via both intrinsic and extrinsic apoptotic pathways. Mechanistically, the CPS induced extrinsic cell apoptosis by upregulating FAS/FASL signaling proteins and cleaved-caspase-8 and promoted a ROS-dependent intrinsic cell apoptosis by activating a JNK and p38 signaling but not ERK1/2 signaling of mitogen-activated protein kinases (MAPK) pathways. These findings provide the first evidence that CPS of M. ovipneumoniae induces a caspase-dependent apoptosis via both intrinsic and extrinsic apoptotic pathways in sheep bronchial epithelial cells, which may be mainly attributed by a ROS-dependent JNK and p38 MAPK signaling pathways.

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Analysis of Carbohydrates and Glycoconjugates by Matrix‐assisted Laser Desorption/Ionization Mass Spectrometry: An Update for 2015–2016

Harvey

2021

Mass Spectrometry Reviews

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This review is the ninth update of the original article published in 1999 on the application of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2016. Also included are papers that describe methods appropriate to analysis by MALDI, such as sample preparation techniques, even though the ionization method is not MALDI. Topics covered in the first part of the review include general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation and arrays. The second part of the review is devoted to applications to various structural types such as oligo‐ and poly‐saccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals. Much of this material is presented in tabular form. The third part of the review covers medical and industrial applications of the technique, studies of enzyme reactions and applications to chemical synthesis. The reported work shows increasing use of combined new techniques such as ion mobility and the enormous impact that MALDI imaging is having. MALDI, although invented over 30 years ago is still an ideal technique for carbohydrate analysis and advancements in the technique and range of applications show no sign of deminishing. © 2020 Wiley Periodicals, Inc.

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Caspase-3 serves as an intracellular immune receptor specific for lipopolysaccharide in oyster Crassostrea gigas

Cited by 56 publications

References 45 publications

Effects of dietary fiber on the antioxidant capacity, immune status, and antioxidant-relative signaling molecular gene expression in rat organs

Effects of dietary fiber on the antioxidant capacity, immune status, and antioxidant-relative signaling molecular gene expression in rat organs

Capsular Polysaccharide of Mycoplasma ovipneumoniae Induces Sheep Airway Epithelial Cell Apoptosis via ROS‐Dependent JNK/P38 MAPK Pathways

Analysis of Carbohydrates and Glycoconjugates by Matrix‐assisted Laser Desorption/Ionization Mass Spectrometry: An Update for 2015–2016

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