Caspase-7 in molar tooth development

Matalová, Eva; Berghe, Tom Vanden; Švandová, Eva; Vandenabeele, Peter; Healy, Christopher; Sharpe, Paul T.; Tucker, Abigail S.

doi:10.1016/j.archoralbio.2012.06.009

Cited by 14 publications

(25 citation statements)

References 26 publications

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“…While normal spermatogenesis is persistently impaired and spermatogenic cells are mostly disappeared, caspase 7 expression in Leydig cells may be activated by caspase 3, caspase 6, and caspase 9 to induce apoptosis. Thus, these findings suggest that the location of caspase 7 may impact on its function, which is similar with the recent reports 131415. However, whether the cleaved form of caspase 7 in germ cells is inevitable during normal spermatogenesis remains unclear.…”

Section: Resultssupporting

confidence: 90%

“…Meanwhile, these observations also revealed that caspase 7 in spermatogenic cells was not combined with caspase 3, caspase 6, and caspase 9 to cause cell apoptosis, which further validated that caspase 7 had nonapoptotic function that participated in normal spermatogenesis. Furthermore, the nonapoptotic functions of caspase 7 were also found in molar tooth development, dental hard tissues formation, and osteogenesis 121314. Unexpectedly, when spermatogenic cells were mostly disappeared at the fourth week after busulfan treatment, IHC results revealed that caspase 7 expression in Leydig cells was significantly elevated, which was positively correlated with the expression of caspase 3, caspase 6, and caspase 9.…”

Section: Discussionmentioning

confidence: 94%

“…The nonapoptotic functions of caspase 3 are observed in bone marrow stem cells and odontogenic epithelial cells 1112. The nonapoptotic functions of caspase 7 are found in tooth development, which play important roles in the differentiation of cells during odontogenesis 1314. Moreover, the activated form of caspase 7 is presented in a number of bone cells, but it is not in connection with apoptosis 15.…”

Section: Introductionmentioning

confidence: 99%

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Apoptotic and nonapoptotic function of caspase 7 in spermatogenesis

Lei

Zhou

et al. 2017

Asian J Androl

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Recent studies have reported that caspase 7 has an apoptotic and nonapoptotic function. However, the relationship between caspase 7 and spermatogenesis remains unknown. This study aimed to investigate the possible function of caspase 7 during normal and abnormal spermatogenesis. The cleaved form of caspase 7 was detected in testis tissues at different postpartum times (5–14 weeks) by qRT-PCR, Western blot and immunohistochemistry (IHC). Then, the mice models of spermatogenic dysfunction were obtained by busulfan (30 mg kg−1 to further evaluate the potential function and mechanism of caspase 7. qRT-PCR and Western blot results showed that caspase 7 expression was gradually elevated from 5 to 14 weeks, which was not connected with apoptosis. IHC results revealed that caspase 7 was mainly located in spermatogenic cells and Leydig cells. In addition, spermatogenic dysfunction induced by busulfan gradually enhanced the apoptosis and elevated the expression of caspase 3, caspase 6, and caspase 9, but decreased the expression of caspase 7 in spermatogenic cells. However, when spermatogenic cells were mostly disappeared at the fourth week after busulfan treatment, caspase 7 expression in Leydig cells was significantly increased and positively correlated with the expression of caspase 3, caspase 6, and caspase 9. Therefore, these results indicate that caspase 7 has a nonapoptic function that participates in normal spermatogenesis, but also displays apoptotic function in spermatogenic dysfunction.

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Section: Resultssupporting

confidence: 90%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Apoptotic and nonapoptotic function of caspase 7 in spermatogenesis

Lei

Zhou

et al. 2017

Asian J Androl

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“…Nuclear expression of executioner caspase-7 has been reported to indicate active apoptotic functions during osteogenesis of tooth and jaw development and within the primary enamel knot (Leist & Jäättelä, 2001;Matalova, Vanden Berghe, et al, 2012;Miura, 2012;Svandova et al, 2014). Similarly, our results demonstrated prevalent nuclear caspase-7 expression during ductal formation, especially near presumptive lumenal areas.…”

Section: Discussionsupporting

confidence: 68%

Apoptosis-associated protein expression in human salivary gland morphogenesis

Teshima

Ianez

Coutinho‐Camillo

et al. 2016

Archives of Oral Biology

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Objective: Salivary gland (SG) development is based on branching morphogenesis, in which programmed cell death has been proposed to play a role in cell signalling and organ shaping.In the mouse salivary gland apoptosis has been suggested to play a key role in lumen formation, removing the central cells of the epithelial stalks. Here we analyse the expression of several anti-and pro-regulators of apoptosis during human SG development in a range of developmental stages.Design: Foetal SGs obtained from the University of São Paulo were analysed by immunohistochemistry to assess the expression of apoptosis-associated proteins: caspases (caspase-6, -7, -9 and cleaved caspase-3), Bcl-2 family members (Bax, Bak, Bad, Bid, Bcl-2, Bcl-x and Bcl-xL), Survivin (BIRC5), Cytochrome C and Apaf-1.Results: Nuclear expression of Bax and Bak was identified in presumptive luminal areas at initial stages, while Bcl-xL showed the most relevant anti-apoptotic activity. Caspase-6, -7 and -9 were expressed during all stages, while interestingly cleaved caspase-3 showed no prominent expression, indicating that caspase-7 is the main effector. Apoptosome complex components Apaf-1 and Cytochrome C, as well as survivin were all positive in developing glands. Conclusions:The particular expression pattern of several apoptotic regulators in human SG development suggests the existence of a fundamental role for apoptosis during duct formation. The absence of Bad and Bid expressions indicates that the instrinsic pathway is more active then the extrinsic during human gland formation. The subcellular localisation of intrinsic-apoptosis proteins correlated with apoptotic activity, but also suggested additional non-apoptotic functions. Highlights: Caspase-7 is the main executioner caspase involved in human SG duct development  Consistent cytoplasmic expression suggest additional non-apoptotic functions  Intrinsic-type apoptotic pathway drives human SG development

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“…The biochemistry of apoptotic events in cells has been well investigated (Hengartner, ). Apoptotic cells are observed in the developmental stages of several ectodermal organs, such as in hair cycling (Paus, ), tooth germ morphogenesis (Jernvall, Aberg, Kettunen, Keranen, & Thesleff, ; Kim et al., ; Matalova et al., ), and tubulogenesis in mammary and salivary glands (Teshima, Wells, Lourenco, & Tucker, ; Watson, ). Additionally, selective cell death is a critical event in the morphogenesis of three‐dimensional (3D) epithelial cell culture models using Madin‐Darby canine kidney (MDCK) cells and MCF‐10A mammary epithelial cells (Debnath et al., ; Saelman, Keely, & Santoro, ).…”

Section: Introductionmentioning

confidence: 99%

Involvement of selective epithelial cell death in the formation of feather buds on a bioengineered skin

2019

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Selective cell death by apoptosis plays important roles in organogenesis. Apoptotic cells are observed in the developmental and homeostatic processes of several ectodermal organs, such as hairs, feathers, and mammary glands. In chick feather development, apoptotic events have been observed during feather morphogenesis, but have not been investigated during early feather bud formation. Previously, we have reported a method for generating feather buds on a bioengineered skin from dissociated skin epithelial and mesenchymal cells in three‐dimensional culture. During the development of the bioengineered skin, epithelial cavity formation by apoptosis was observed in the epithelial tissue. In this study, we examined the selective epithelial cell death during the bioengineered skin development. Histological analyses suggest that the selective epithelial cell death in the bioengineered skin was induced by caspase‐3‐related apoptosis. The formation of feather buds of the bioengineered skin was disturbed by the treatment with a pan‐caspase inhibitor. The pan‐caspase inhibitor treatment suppressed the rearrangement of the epithelial layer and the formation of dermal condensation, which are thought to be essential step to form feather buds. The suppression of the formation of feather buds on the pan‐caspase inhibitor‐treated skin was partially compensated by the addition of a GSK‐3β inhibitor, which activates Wnt/β‐catenin signaling. These results suggest that the epithelial cell death is involved in the formation of feather buds of the bioengineered skin. These observations also suggest that caspase activities and Wnt/β‐catenin signaling may contribute to the formation of epithelial and mesenchymal components in the bioengineered skin.

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Caspase-7 in molar tooth development

Cited by 14 publications

References 26 publications

Apoptotic and nonapoptotic function of caspase 7 in spermatogenesis

Apoptotic and nonapoptotic function of caspase 7 in spermatogenesis

Apoptosis-associated protein expression in human salivary gland morphogenesis

Involvement of selective epithelial cell death in the formation of feather buds on a bioengineered skin

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