Caste-based differences in gene expression in the polyembryonic wasp Copidosoma floridanum

Hexapoda: Comparative Aspects of Later Embryogenesis and Metamorphosis

“…Reproductive morphs follow a typical holometabolous life cycle. Their sibling defender morphs have an active fi rst instar with enlarged mandibles, but die without molting (Donnell and Strand 2006 ). Polyembryonic development and caste determination have been studied in Copidosoma fl oridanum .…”

Analysis of odorant-binding protein gene family members in the polyembryonic wasp, Copidosoma floridanum: Evidence for caste bias and host interaction

“…Full length sequences for two of the genes described here (CfloOBP1 and CfloOBP2) were previously isolated along with expressed sequence tags (ESTs) for another 6 OBP genes in a study to isolate genes differentially expressed in castes of Copidosoma (Donnell and Strand, 2006). The sequences were isolated using the PCR-based suppression subtractive hybridization (SSH) procedure described by Diatchenko et al (1996).…”

“…Using a bi-directional suppression subtractive hybridization (SSH) approach, Donnell and Strand (2006) identified genes differentially expressed in either of the larval castes. They obtained expressed sequence tags (ESTs) for genes encoding a large array of putative proteins with potential caste-specific functions including members of the odorant-binding protein (OBP) gene family.…”

“…The variation in OBP gene numbers invites questions regarding the role of OBPs in chemical communication and in other processes within insects (Leal, 2013). As a preliminary step towards ascertaining the role of OBPs within the caste structure of Copidosoma, a more detailed analysis of the group of 8 ESTs identified as putative OBPs in the original study of Donnell and Strand (2006) was undertaken. Complete cDNA sequences were obtained for the 6 OBP gene family members not already available.…”

Identification of genes expressed in the accessory glands of male Mediterranean Fruit Flies (Ceratitis capitata)

“…This technique has been used with success to isolate genes expressed in different social insect castes (e.g. Donnell and Strand, 2006), in specific insect tissues and to isolate Acps in D. melanogaster (e.g. Wolfner et al, 1997).…”