Catalpol Attenuates IL-1β Induced Matrix Catabolism, Apoptosis and Inflammation in Rat Chondrocytes and Inhibits Cartilage Degeneration

Abstract

BackgroundChondrocyte dysfunction and apoptosis are 2 major features during the progression of osteoarthritis. Catalpol, an iridoid glycoside isolated from the root of Rehmannia, is a valuable medication with anti-inflammatory, anti-oxidative, and anti-apoptotic effects in various diseases. However, whether catalpol protects against osteoarthritis has not been investigated.Material/MethodsTo assess the role of catalpol in osteoarthritis and the potential mechanism of action, chondrocytes were treated with interleukin (IL)-1β and various concentrations of catalpol. Catabolic metabolism, apoptotic level and relative signaling pathway were measured by western blot, real-time polymerase chain reaction and immunofluorescence staining. Meanwhile, we assess the cartilage degeneration in an experimental rat model using Safranin O fast green staining and cartilage was graded according to the Osteoarthritis Research Society International (OARSI) system.ResultsThe results showed that catalpol prevented chondrocyte apoptotic level triggered by IL-1β, suppressed the release of catabolic enzymes, and inhibited the degradation of extracellular matrix induced by IL-1β. Catalpol also inhibited the nuclear factor kappa B (NF-κB) pathway, reduced the production of inflammatory cytokines (IL-6, tumor necrosis factor-α) in IL-1β-treated chondrocytes, and partially reversed cartilage degeneration in the knee joint in animal model of osteoarthritis.ConclusionsOur work suggested that catalpol treatment attenuates IL-1β-induced inflammatory response and catabolism in rat chondrocytes by inhibiting the NF-κB pathway, suggesting the therapeutic potential of catalpol for the treatment of osteoarthritis.

Ropivacaine Induce G0 / G1 Phase Cell Cycle Arrest and Apoptosis of PC12 Cells Via Inhibiting Mitochondrial STAT3 Translocation

“…In past decades, a growing body of evidence has revealed that apoptosis plays an important role in the ropivacaine induced neurotoxicity (Chen Y et al, 2019; Wang S et al, 2019;Wen X et al, 2019), most studies have demonstrated that ropivacaine may induce neurotoxicity via activating apoptosis and up-regulating apoptosis-related genes. These were consistent with our previous ndings that ropivacaine could induce apoptosis of PC12 cells which was related to the activation of the Fas / Fasl signaling pathway (Zeng Y et al, 2019).…”

Protective Effects of Hydrogen-Rich Water Against Cartilage Damage in a Rat Model of Osteoarthritis by Inhibiting Oxidative Stress, Matrix Catabolism, and Apoptosis

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Protective effects of catalpol on mitochondria of hepatocytes in cholestatic liver injury

“…Catalpol, which is an iridoid glycoside extracted from Rehmannia glutinosa, has an anti-ischemic effect (18)(19)(20)(21). it has been suggested that catalpol could effectively protect promyelinating oligodendrocytes from apoptosis and myelination defects.…”