Cation Exchange High Performance Liquid Chromatography for Diagnosis of Haemoglobinopathies

Gupta, PK; Kumar, Hemant; Kumar, Santosh; Jaiprakash, M

doi:10.1016/s0377-1237(09)80051-8

Cited by 25 publications

(26 citation statements)

References 17 publications

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“…These conventional methods are family history, clinical features, hemoglobin level, red blood cell indices, red cell count, peripheral blood smear study, Hb A2 quantification, Hb F quantification, cellulose acetate electrophoresis at alkaline pH for hemoglobin and sickling test. Various drawbacks and shortcomings of these methods are that Hb S,G,D,Q and Lepore have same mobility on electrophoresis and Hb A2,C and E also have same mobility [6] . Double heterozygous states also are difficult to diagnose [6] .…”

Section: Introductionmentioning

confidence: 99%

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Variant Hemoglobin Spectrum By Cation Exchange High Performance Liquid Chromatography: A Study 0f 2035 Subjects

Sarvaiya

Chauhan

2017

APALM

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Introduction: Hemoglobinopathies and thalassemia are hereditary disorders of hemoglobin (Hb) affecting mankind at prevalent regional level. Automated cation exchange high performance liquid chromatography is being increasingly used as the initial diagnostic method for identifying normal and abnormal hemoglobin variants. Methodology:Total 2035 sample received and studied. All samples run on cation exchange high performance liquid chromatography machine by BIO-RAD.Results: Total 386(18.96%) cases had abnormal hemoglobin fractions. Beta thalassemia major cases were 17(0.83%). Beta thalassemia intermedia cases were 4(0.19%). Sickle cell trait (heterozygous Hb S) cases were 96(4.71%). Double heterozygous for sickle cell-beta thalassemia cases were 14(0.68%). There were 06 cases (0.29%) of Hb D heterozygous. There were one each case of Hb E heterozygous and Hb E-beta thalassemia (0.04% each). Hb F was raised in 7 adult cases (0.34%). Conclusion:To conclude, cation exchange high performance liquid chromatography is less time consuming, cost effective, labor saving, reproducible, accurate, sensitive and specific method to detect hemoglobinopathies and thalassemia. Most of the abnormal cases are diagnosed with this method, with few inconclusive cases require further genetic and molecular workup.

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Section: Introductionmentioning

confidence: 99%

“…Various drawbacks and shortcomings of these methods are that Hb S,G,D,Q and Lepore have same mobility on electrophoresis and Hb A2,C and E also have same mobility [6] . Double heterozygous states also are difficult to diagnose [6] . Clinical and laboratory features have low specificity.…”

Section: Introductionmentioning

confidence: 99%

Variant Hemoglobin Spectrum By Cation Exchange High Performance Liquid Chromatography: A Study 0f 2035 Subjects

Sarvaiya

Chauhan

2017

APALM

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“…In a study done by Gupta et al mean Hb was found to be 9.3 gm%. 9 Lower hemoglobin in some of our cases could be due to associated iron deficiency ( Table 3). The hemoglobinopathies, particularly β-thalassemias are a considerable health problem.…”

Section: Discussionmentioning

confidence: 81%

Prevalence of haemoglobinopathies in anemic females

et al. 2016

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“…Mean Hb of antenatal group was found to be 9.1 gm % which was slightly lower than the student group 10.9 gm %. In a study done by Gupta et al [17] mean Hb was found to be 9.3 gm%. Lower hemoglobin in some of our cases could be due to associated iron deficiency.…”

Section: Discussionmentioning

confidence: 87%

Morphological Patterns of Anaemia and Prevalence of Haemoglobinopathy in State of Haryana

Singh¹,

Sen

Singh

et al. 2018

APALM

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Background: Hemoglobinopathies including thalassemias are important prevalent group of erythrocytic genetic disorders found in various parts of the world and are important cause of morbidity and mortality.Methods: Samples were run on 5 part differential cell counter and after analyzing various RBC parameters and peripheral smear, the appropriate sample on biorad HPLC for hemoglobinopathy and mutation study was done. Statistical analysis performed. Study population included two groups-anemic pregnant women and students.Result: Out of 361 cases; 27 were positive for haemoglobinopathies giving a prevalence of 7.5 % in anemic mothers. Positive cases included 22 cases of β-thalassemia trait, 2 HbS trait, 1 case of HbD trait, 1 case of HbE trait and 1 case of HbE homozygous. Out of a total of 630 cases, 38 were found positive for various haemoglobinopathies including 36 cases of β-thalassemia trait, 1 case of thalassemia intermedia and 1 case of HbLepore. DNA analysis was done for 10 cases in which the common mutation found was IVSI-5 followed by codon 41/42 and codon 8/9Conclusion: Proper screening studies should be done to diagnose the cases and reduce the prevalence of thalassemia major by informing and educating such cases.

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Cation Exchange High Performance Liquid Chromatography for Diagnosis of Haemoglobinopathies

Abstract: The simplicity of the sample preparation, superior resolution of the method and accurate quantitation of haemoglobin concentration, combined with complete automation, makes this an ideal methodology for diagnosis of haemoglobinopathies.

Cited by 25 publications

References 17 publications

Variant Hemoglobin Spectrum By Cation Exchange High Performance Liquid Chromatography: A Study 0f 2035 Subjects

Variant Hemoglobin Spectrum By Cation Exchange High Performance Liquid Chromatography: A Study 0f 2035 Subjects

Prevalence of haemoglobinopathies in anemic females

Morphological Patterns of Anaemia and Prevalence of Haemoglobinopathy in State of Haryana

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